Malachite green reagent was prepared by separately dissolving 34 mg malachite green carbinol base (Sigma) in 40 mL of 1 N HCl and 1 g of ammonium molybdate (Sigma) in 15 mL of 4 N HCl. Solutions were vortexed and placed on a rotational shaker for 1 h at room temperature. The volume of each solution was increased to 50 mL using H2O, and then combined and filtered through a 0.22 μm filter. The amount of reagent prepared was scaled as appropriate.
Malachite green carbinol base
Manufactured by Merck Group
Sourced in Singapore
Malachite green carbinol base is a chemical compound that serves as a versatile laboratory reagent. Its primary function is as a colorimetric indicator, used to detect and quantify the presence of various analytes in a sample. This compound exhibits a distinct color change in response to pH variations, making it a useful tool for analytical applications.
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2 protocols using malachite green carbinol base
1
Malachite Green Reagent Preparation
2
Malachite Green Aptamer Binding Assay
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RNA aptamer (MGA) (5'-GGAUCCCGACUGGCGAGAGCCAGGUAACGAAUGGAUCC-3') was purchased from AITbiotech Pte Ltd Singapore. Malachite green oxalate (MG) and malachite green carbinol base (MGOH) were purchased from Sigma Aldrich, Singapore.
Dimethyl sulfoxide (DMSO) was purchased from Alfa Aesar, USA. Ethanol (EtOH)
was purchased from Fisher Scientific, Singapore. HPLC grade acetonitrile (ACN) was purchased from Tedia, USA. Polystyrene microplates (96-well) were purchased from Corning, USA. All chemicals and materials were used as received.
Dimethyl sulfoxide (DMSO) was purchased from Alfa Aesar, USA. Ethanol (EtOH)
was purchased from Fisher Scientific, Singapore. HPLC grade acetonitrile (ACN) was purchased from Tedia, USA. Polystyrene microplates (96-well) were purchased from Corning, USA. All chemicals and materials were used as received.
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