Log In Sign up
The largest database of trusted experimental protocols

Psmb5

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

PSMB5 is a core subunit of the 20S proteasome, which is a multicatalytic proteinase complex responsible for the degradation of most intracellular proteins. PSMB5 is involved in the proteolytic activities of the proteasome.

Automatically generated - may contain errors

Lab products found in correlation

Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Mcl 1, by Rockland Immunochemicals (1 mentions) Cyp2e1, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) β actin, by Merck Group (1 mentions) Smarcb1 snf5, by Fortis Life Sciences (1 mentions) Cyclin b1, by Cell Signaling Technology (1 mentions) Ire1 alpha, by Cell Signaling Technology (1 mentions) Baf155, by Cell Signaling Technology (1 mentions) Baf170, by Santa Cruz Biotechnology (1 mentions) Baf60a, by Santa Cruz Biotechnology (1 mentions) Ube2c, by Proteintech (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Ripa buffer, by Cell Signaling Technology (1 mentions) Phosstop, by Roche (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) β actin c4, by Santa Cruz Biotechnology (1 mentions) Smarca4, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Rabbit anti-PSMB5 (3 citations)

4 protocols using psmb5

1

Antibody Characterization for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study were β-actin (#A5441) from Sigma-Aldrich, Cleaved Caspase 3 (#9661L) and RIP1 (#3493) from Cell Signaling, RIP3 (#2283) from ProSci, CYP2E1 (#Ab19140), p65 (#sc-372) from Santa Cruz Biotechnology, PSMA2 (#Ab109525) and PSMB5 (#Ab3330) from Abcam, PSMC1(#A303-821A) from Bethyl, anti-lymphocyte antigen B superfamily (Ly6B, #MCA771G) from AbD Serotec, MCL-1 (#600-401-394) from Rockland. Horseradish peroxidase—conjugated secondary antibodies were from Jackson ImmunoResearch Laboratory. Ethanol was from Pharmaco, RIP1 Inhibitor 7-Cl-O-Nec-1 (7-Nec1, 504297) from Calbiochem and all other chemicals were from Sigma, Invitrogen or Calbiochem.
Wang S., Ni H.M., Dorko K., Kumer S.C., Schmitt T.M., Nawabi A., Komatsu M., Huang H, & Ding W.X. (2016). Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget, 7(14), 17681-17698.
+ Open protocol
+ Expand
2

Immunoblotting of SWI/SNF Complex

Check if the same lab product or an alternative is used in the 5 most similar protocols
After indicated treatments, cell lysates were harvested using RIPA buffer (Cell Signaling Technologies) with protease inhibitors (cOmplete, Roche) and phosphatase inhibitors (PhosSTOP, Roche). Antibodies used were as follows: ARID1A (Santa Cruz; sc-373784), α-tubulin (Santa Cruz; sc-5286), β-Actin (C-4) (Santa Cruz; sc-47778), β-Actin (Cell Signaling; 8457), BAF57/SMARCE1 (Bethyl Laboratories, A300-810A), BAF60a (Santa Cruz; sc-135843), BAF155 (Cell Signaling; 11956), BAF170 (Santa Cruz; sc-166237), SMARCA4 (Santa Cruz; 17798), Cleaved Caspase-3 (Cell Signaling; 9664), c-MYC (Santa Cruz; sc-764) or c-MYC (Cell Signaling; 9402), cyclin B1 (Cell Signaling; 4135 and 4138), cyclin D1 (Santa Cruz; sc-718), GAPDH (Cell Signaling; 2118S and 97166S), GRP78 (Rockland Antibodies, Limerick, PA; 200–301 F36), IRE1-alpha (Cell Signaling; 3294), lamin A/C (Cell Signaling; 2032), PSMB5 (Abcam, Cambridge, MA; ab3330), SMARCB1/SNF5 (Bethyl A301-087A), UBE2C (Proteintech, Rosemont, IL; 66087–1). Results shown are representative of at least two biological replicates.
Hong A.L., Tseng Y.Y., Wala J.A., Kim W.J., Kynnap B.D., Doshi M.B., Kugener G., Sandoval G.J., Howard T.P., Li J., Yang X., Tillgren M., Ghandi M., Sayeed A., Deasy R., Ward A., McSteen B., Labella K.M., Keskula P., Tracy A., Connor C., Clinton C.M., Church A.J., Crompton B.D., Janeway K.A., Van Hare B., Sandak D., Gjoerup O., Bandopadhayay P., Clemons P.A., Schreiber S.L., Root D.E., Gokhale P.C., Chi S.N., Mullen E.A., Roberts C.W., Kadoch C., Beroukhim R., Ligon K.L., Boehm J.S, & Hahn W.C. (2019). Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition. eLife, 8, e44161.
+ Open protocol
+ Expand
3

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were extracted and concentrations were determined using the DC assay (Bio‐Rad, Hemel Hempstead, UK) and 15 µg of protein was separated using a 10% bis‐tris gel and then transferred to a nitrocellulose membrane and probed with goat anti‐mouse E11 (1:1000, R&D Systems) and HRP‐linked rabbit anti‐goat secondary antibody (1:3,000, Dako, Cambridge, UK); or rabbit anti‐mouse proteasome subunit beta type‐5 (PSMB5) (1:1,000, Abcam) and HRP‐linked goat anti‐rabbit secondary antibody (1:3,000, Dako); or rabbit anti‐mouse RhoA (1:1,000, Cell signaling) and HRP‐linked goat anti‐rabbit secondary antibody (1:3,000, Dako) diluted in 5% non‐fat milk. Rabbit anti‐mouse ERM and phospho‐ERM (1:1000, Invitrogen) antibodies and HRP‐linked goat anti‐rabbit secondary antibody (1:3,000, Dako) were diluted in 3% bovine serum albumin.
Immune complexes were visualised by chemiluminescence using the ECL detection kit and ECL film (GE Healthcare, Amersham, UK). HRP‐conjugated anti β‐actin antibody (1:25,000, Sigma) was used as a loading control.
Staines K.A., Prideaux M., Allen S., Buttle D.J., Pitsillides A.A, & Farquharson C. (2015). E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404.
+ Open protocol
+ Expand
4

Quantifying Protein Expression by Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell cultures were treated with RIPA buffer. Protein extracts were separated in 12.5% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), transferred onto nitrocellulose membranes (Sigma) and immunoblotted with the antibodies against PSMB5 (Abcam) and actin ( Abcam). Protein bands were visualized with enhanced chemiluminescence (Millipore).
Wei W., Zou Y., Jiang Q., Zhou Z., Ding H., Yan L, & Yang S. (2018). PSMB5 is associated with proliferation and drug resistance in triple-negative breast cancer. The International journal of biological markers, 33(1).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!