To detect the expression level of caspase-3 in GSCs, 2 × 104 cells were seeded in each well of a 96 wells plate then the cells were treated with 16 μg/ml of G. densiflorum-Ag/AgCl-NPs for 48 h. Then the expression level of caspase-3 protein was detected by fluorescence microscope followed by the treatment with 4% paraformaldehyde, 0.1% Triton X-100, phosphate buffer saline, 10% goat serum, 0.5% tween-20, and 1% BSA in PBS and finally, caspase-3 primary antibody and Cy3 goat-anti Rabbit IgG antibody (Life technology) followed by the protocol described by Kabir et al.1.
Cy3 goat anti rabbit igg antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Cy3 goat-anti Rabbit IgG antibody is a fluorescently labeled secondary antibody that binds to rabbit primary antibodies. It is commonly used in fluorescence-based detection and imaging techniques.
Automatically generated - may contain errors
2 protocols using cy3 goat anti rabbit igg antibody
1
Detecting Caspase-3 Expression in GSCs
2
Apoptosis Assay in Cell Cultures
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DMEM, DMEM/F12 medium (1:1), Laminin, Foetal bovine serum purchased from Gibco, MTS from Promega (USA), FITC‐Annexin V/PI from ebioscience (USA), Primary caspase‐3 antibody from Cell Signalling, Cy3 goat antirabbit IgG antibody from Life technology; RT master mix and SYBR green master mix from Applied Biosystem; Primer from TsingKe Biological Technology China; Silver nitrate (Scharlau, Spain). Highest analytical grades of chemicals were used throughout the experiments.
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