Thunderbird probe and sybr qpcr mix

Manufactured by Toyobo

Sourced in Germany, Japan

THUNDERBIRD Probe is a real-time PCR reagent for probe-based detection. SYBR qPCR Mix is a real-time PCR reagent for SYBR Green-based detection. Both products are designed for use in quantitative PCR (qPCR) applications.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (1 mentions) Revertra ace qpcr rt master mix, by Toyobo (1 mentions) Lightcycler 96 system, by Roche (1 mentions) Gdna remover, by Toyobo (1 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Merck Group (1 mentions) Cfx384 touch real time pcr detection system, by Bio-Rad (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Facsaria iiu, by BD (1 mentions) Collagenase b, by Roche (1 mentions)

Close spelling variants of this product

THUNDERBIRD Probe qPCR Mix (171 citations) SYBR qPCR Mix (122 citations) THUNDERBIRD qPCR Mix (106 citations) Thunderbird SYBR mix (5 citations) THUNDERBIRD Probe (3 citations) THUNDERBIRD SYBR qPCR (2 citations)

3 protocols using thunderbird probe and sybr qpcr mix

Quantifying Retroviral and Epigenetic Markers

Cited 1 time

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LINE-1, human endogenous retrovirus (HERVK14C), and SVA were assessed using cDNA prepared from the patients’ blood samples by quantitative polymerase chain reaction (qPCR), as previously described^{26 (link),49 (link)}. To examine the expression of methylation enzymes, including IFNs, JAK family members, TFs, and ISGs, qPCR was performed using specific primer pairs (Table S9). In brief, qPCR was performed using a THUNDERBIRD Probe and SYBR qPCR Mix (Toyobo). The expression level relative to that of a housekeeping gene was used in the analyses. Real-time PCR was performed using an ABI 7300 PCR thermal cycler (Applied Biosystems) under the following conditions: 10 min at 95 °C, 40 cycles of 15 s at 95 °C, and 1 min at 60 °C. The data were analyzed using the ΔΔCt method.

Kuriyama Y., Shimizu A., Kanai S., Oikawa D., Motegi S.I., Tokunaga F, & Ishikawa O. (2021). Coordination of retrotransposons and type I interferon with distinct interferon pathways in dermatomyositis, systemic lupus erythematosus and autoimmune blistering disease. Scientific Reports, 11, 23146.

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Comparative qPCR Analysis of bla and rrsA

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RT-qPCR analysis was conducted to compare the transcription of bla_IMP-6 with that of rrsA. Total RNA was extracted using the RNeasy Mini Kit (Qiagen) after incubation in LB until the optical density at 600 nm (OD₆₀₀) reached 0.4–0.6. RNA was treated with ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Life Science) to remove contaminating DNA and to reverse-transcribe the RNA to cDNA. rrsA encoding the 16S ribosomal RNA served as an endogenous control for normalization. qPCRs were carried out using the Thunderbird Probe and SYBR qPCR Mix (Toyobo Life Science) on a LightCycler 96 System (Roche, Penzberg, Germany). The primers used in this assay are listed in Table S3. qPCR analysis was performed using data from repeated experiments (n = 5), and transcript levels were calculated from Ct values using the comparative Ct method (15 (link)).

Abe R., Akeda Y., Sugawara Y., Matsumoto Y., Motooka D., Iida T, & Hamada S. (2023). Carbapenem triggers dissemination of chromosomally integrated carbapenemase genes via conjugative plasmids in Escherichia coli. mSystems, 8(3), e01275-22.

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Isolation and Characterization of Mouse Germ Cells

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Mouse cDNA was prepared from various tissues of adult mice, or from 5- to 42-day-old mouse testes. Alternatively, cDNA was prepared from spermatocytes and spermatids, which were isolated and purified from mouse testes as previously described^{43 (link)}. Briefly, testes from 24-day-old mice were dissected to remove the tunica albuginea. The decapsulated testes were treated with collagenase B (Roche, Tokyo, Japan) and DNase I (Sigma-Aldrich, St. Louis, MO), followed by treatment with trypsin (ThermoFisher, Waltham, MA) and DNase I. The isolated germ cells were then washed with phosphate-buffered saline (PBS) and stained with Hoechst 33342 in 5% bovine serum albumin (BSA) in PBS at 34°C for 1 hour. Spermatocytes and spermatids were sorted based on DNA content and light-scattering properties by BD FACSAria™ IIu (BD Biosciences, Tokyo, Japan). Reverse transcription quantitative PCR (RT-qPCR) was performed using the THUNDERBIRD™ Probe and SYBR^® qPCR Mix (TOYOBO, Osaka, Japan) and the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA). The sequences of primers are listed in Table S2.

Lu Y., Nagamori I., Kobayashi H., Kojima-Kita K., Shirane K., Chang H.Y., Nishimura T., Koyano T., Yu Z., Castañeda J.M., Matsuyama M., Kuramochi-Miyagawa S., Matzuk M.M, & Ikawa M. (2023). ADAD2 functions in spermiogenesis and piRNA biogenesis in mice. Andrology, 11(4), 698-709.

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