1H and 13C NMR spectra of each compound were recorded in commercially available deuterated solvents on a Bruker Avance III DPX 400 (1H at 400 MHz, 13C at 101 MHz). All chemical shifts are given in ppm and coupling constants are in Hz.
Avance 3 dpx 400
Manufactured by Bruker
The Avance III DPX 400 is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for analytical applications. It provides a magnetic field strength of 400 MHz and is equipped with a digital signal processing system for efficient data acquisition and processing.
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Lab products found in correlation
3 protocols using avance 3 dpx 400
1
NMR Spectroscopy of Compounds
2
Proton NMR Spectroscopy of Polymer Blends
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Proton (1H) nuclear magnetic resonance (NMR) spectra were measured at 400 MHz on a Bruker Avance III DPX 400. Approximately 100 mg of each sample were dissolved in 500 μL deuterated dimethylsulfoxide (DMSO-d6) for analysis. To evaluate the ratios of PAA:PU and NHS:PAA, each spectrum was normalized based on the peak around 2.22 ppm attributed to the methylene peak in PAA. The peaks at 7.06 ppm, attributed to the nitrogen-attached hydrogen in PU, were integrated with respect to the normalized peaks to approximate the molar ratios of PAA:PU in the final purified products (Supplementary Fig. 5 ). The NHS alkyl peaks at 2.76 ppm were integrated with respect to the normalized peaks to estimate the degree of NHS functionalization (Fig. 2d ).
3
Synthetic Procedures for Novel Compounds
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The syntheses used in this study involve: 1) carbodiimide-mediated coupling reactions to form amide linkages, 2) conventional deprotection reactions of tert-butyloxycarbonyl (Boc), and 3) hydrolysis of ester functionalities to produce carboxylic acid moieties. As the only exception, the zwitterionic head group of 2 is obtained by quaternization of a tertiary amine with a propanesultone. 1 H and 13 C nuclear magnetic resonance (NMR, Supplementary Section S2a, Bruker Avance III DPX 400) and mass spectrometry (MS, Supplementary Section S2b, Bruker Omniflex) were used to confirm the chemical composition of intermediates and products. The observed solubilities of 1 and 2 are up to 1.0 mg/mL, and the solubility of 3 exceeds 20 mg/mL. Synthesis details on each of compounds 1, 2, 3, OEG-AA, and the FRET donor-and quencher-labeled AAs and their intermediates are provided in Supplementary Section 1.
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