Cortisol hydrocortisone

DSP (Dithiobis [Succinimidyl Propionate]), which is the linker used in this research, its solvent Dimethyl Sulfoxide (DMSO) and PBS (Phosphate buffered saline) were ordered from Thermofisher scientific Inc. (Waltham, MA, USA). Human serum was also purchased from Thermofisher scientific. Cortisol (Hydrocortisone) and the monoclonal α-cortisol antibody were ordered from Abcam (Cambridge, MA, USA). Whole blood was collected from Carter blood care (Plano, TX, USA). Human sweat was obtained from Lee bisolutions Inc. (St. Louis, MO, USA). BMIM[BF4] was purchased from Sigma-Aldrich (St. Louis, Mo, USA). Milipore DI water (Conductivity-18 MΩ.cm) was used for making the dilutions and the buffers. The antibody was diluted in BMIM[BF₄].

Polyamide substrates were obtained from GE Healthcare Lifesciences (NJ, USA). Linker and solvents, namely DSP (Dithiobis [Succinimidyl Propionate]), DMSO (Dimethyl Sulfoxide) and PBS (phosphate-buffered saline), were purchased from Thermofisher Scientific, Inc. (MA, USA). Cortisol (Hydrocortisone) and capture probe (α-cortisol antibody) were obtained from Abcam (MA, USA). Synthetic sweat was prepared in Mili-Q pore deionized water (18 MΩ) according to the protocol described in Mathew et al. [12 ]. Human subject participation was conducted in accordance with the protocol approved by IRB at the University of Texas at Dallas.