Rat cardiac troponin 1 simplestep elisa kit

Cardiac troponin I was quantitatively measured with the use of Rat Cardiac Troponin I SimpleStep ELISA Kit (Abcam, Cambridge, United Kingdom) in coronary effluents. Troponin I was tied with antibody specific to rat cardiac muscle troponin I and was detected by biotin-conjugated polyclonal antibody and HRP. Next, TMB Development Solution was used to enable visualization of the reaction. A minimum detectable dose of rat cardiac TnI was 7.7 pg/mL. TnI concentration in coronary effluents was expressed as pg/mL and normalized to CF.

The concentration of ventricular isoform of myosin light chain 1 (MLC1) in perfusates was determined using Rat Myosin Light Chain 3 ELISA Kit (Bioassay Technology Laboratory, Shanghai, China). Briefly, primary capture antibodies bind MLC1 from the sample. Then, MLC1 is detected with biotinylated anti-MLC1 secondary antibodies and streptavidin-horseradish peroxidase (HRP) complex. The substrate solution is then added, and color develops in proportion to the amount of rat MLC1.
Cardiac troponin I (TnI) in coronary effluents was quantitatively measured using Rat Cardiac Troponin I SimpleStep ELISA Kit (Abcam, Cambridge, UK). Briefly, TnI is tied with antibody specific to rat cardiac muscle TnI and is detected by biotin-conjugated polyclonal antibody and HRP. Next, TMB Development Solution is used to enable visualization of the reaction. The concentration of MLC1 and TnI in coronary effluents was normalized to CF.