Poloxamer 407 plx 407

THC (98%) was purchased from Carbosynth Ltd. (Compton, UK). κc and XG powder were commercially obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Hydroxypropyl methylcellulose (HPMC E15, HPMC K4M and HPMC K15M), polyvinyl alcohol (PVA, 98 KDa) and methylcellulose (MC) were purchased from Zhengzhou Tianying Chemicals Co., Ltd. (Yixing, China). Polyvinyl pyrrolidone K25 (PVP-K25, 30 KDa), poloxamer 188 (PLX-188) and poloxamer 407 (PLX-407) were obtained from BASF (Ludwigshafen, Germany). Oral squamous cell carcinoma (OSCC) was obtained from the American Type Culture Collection (ATCC). 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) was purchased from Serva (Heidelberg, Germany). Penicillin and streptomycin solutions (100 U/mL each) were purchased from BioWhittaker® (Lonza, Belgium). The carcinogen 7, 12-Dimethylbenz[a]anthracene (DMBA; > 95%), Dulbecco’s modified eagle medium (DMEM), Fetal Bovine Serum (FBS) and bovine serum albumin were purchased from Sigma Aldrich Chemie GmbH (Steinheim, Germany). Cyclin D1 was purchased from Thermo Scientific (Waltham, MA, USA). Absolute ethanol, sorbitol, and mannitol were obtained from ADWIC (Cairo, Egypt). All other reagents and chemicals are analytical grade.

SUT was purchased from Natco Fine Pharmacis Pvt Ltd (Hyderabad, India). Soybean phospholipids, cholesterol, tween 80, sodium caprate, and sodium cholate were purchased from Aladdin (Shanghai, China). Poloxamer 407 (PLX 407), poloxamer 188 (PLX 188), and carrageenan were purchased from (BASF, Ludwigshafen am Rhein, Germany). Clostridium perfringens enterotoxin (CPE) was purchased from MyBiosource, Inc. (Southern California, San Diego, CA). Acetonitrile, methanol, and chloroform were provided by Aladdin. All reagents were of high-performance liquid chromatography (HPLC) or analytical grade. Rabbits and rats were purchased from the animal house of the Faculty of Medicine, Assiut University, Egypt.