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Cncs compartmental protein extraction kit

Manufactured by Merck Group
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The CNCS Compartmental Protein Extraction Kit is a laboratory equipment product designed for the isolation and purification of proteins from biological samples. It employs a compartmental approach to selectively extract proteins from different cellular fractions, facilitating the study of protein localization and function within the cellular environment.

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Lab products found in correlation

Ab52178, by Abcam (2 mentions) Ab765p, by Merck Group (2 mentions) Bullet blender, by Next Advance (2 mentions)

2 protocols using cncs compartmental protein extraction kit

1

Tumor Extracellular Protein Enrichment

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Samples described above were mechanically disrupted using a Bullet Blender (Next Advance). Tumor lysates were then subjected to incubation in a series of buffer (CNCS Compartmental Protein Extraction Kit, Millipore) resulting in the extraction of soluble and mostly intracellular components and the enrichment for insoluble extracellular proteins (for more details, see 63 (link),64 (link)). The decellularization efficiency was monitored by western blot analysis monitoring the depletion of collagen I (Millipore, AB765P), actin (rabbit polyclonal antibody 14–4 generated in the Hynes lab at MIT), and histone H4 (Abcam, ab52178) across the 4 sequential extraction steps (see Supplementary Figure 2B, lanes 2 to 5).
Di Martino J.S., Nobre A.R., Mondal C., Taha I., Farias E.F., Fertig E.J., Naba A., Aguirre-Ghiso J.A, & Bravo-Cordero J.J. (2021). A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy. Nature cancer, 3(1), 90-107.
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2

Tumor Extracellular Protein Enrichment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples described above were mechanically disrupted using a Bullet Blender (Next Advance). Tumor lysates were then subjected to incubation in a series of buffer (CNCS Compartmental Protein Extraction Kit, Millipore) resulting in the extraction of soluble and mostly intracellular components and the enrichment for insoluble extracellular proteins (for more details, see 63 (link),64 (link)). The decellularization efficiency was monitored by western blot analysis monitoring the depletion of collagen I (Millipore, AB765P), actin (rabbit polyclonal antibody 14–4 generated in the Hynes lab at MIT), and histone H4 (Abcam, ab52178) across the 4 sequential extraction steps (see Supplementary Figure 2B, lanes 2 to 5).
Di Martino J.S., Nobre A.R., Mondal C., Taha I., Farias E.F., Fertig E.J., Naba A., Aguirre-Ghiso J.A, & Bravo-Cordero J.J. (2021). A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy. Nature cancer, 3(1), 90-107.
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