Imb spss v22

Statistical analyses were performed using the commercial IMB SPSS v22 software package (IBM Corporation, Armonk, NY).The sample size was calculated based on the LEMS score as the main variable, considering a mean difference of 8.2 and a standard deviation of 10 that was obtained from a pilot study [32 (link)]. To achieve a Type I error (α) of 0.05 and a power of 80% a sample size of 24 subjects in each group is needed. The homogeneity between the groups in terms of age, gender, time since injury, WISCI-II and LEMS was evaluated with a Levene´s test for equality of variances. A two-way repeated-measures ANOVA, with the “time” factor (baseline and post-intervention) and the “group” factor (IG and CG groups), was performed to compare differences in the motor score (LEMS) and the functional gait variables (10MWT, TUG, 6MWT, WISCI-II and SCIM-III). The Greenhouse–Geisser correction was applied to correct for non-sphericity, and a Bonferroni post-hoc multiple comparison test was used to highlight specific differences between evaluation time and groups. Participants who did not complete the study were not included in the statistical analysis. A p-value ≤ 0.05 was considered statistically significant.

The measurements reported for protein particle size, degree of hydrolysis, and trypsin inhibitory activity are the results of three independent measurements, while two independent measurements were used to estimate the molecular weight distribution and free amino acids. Statistical analysis was performed using IMB SPSS v22 (Armonk, IBM Corp, New York, NY, USA), and minimum significance was set at the 5% level (p < 0.05). One way analysis of variance (ANOVA) with Tukey HSD was applied to trypsin inhibitory activity and free amino acids and was conducted to determine the significance of the treatments, while a two-way multivariate analysis of variance (MANOVA) was applied to SEC-HPLC Mw distribution.