Presence and absence of DHT, we stained AR expressing K4DT DPCs with HA antibody. We obtained 16 cell images from each group. Based on the staining feature, we counted the number of cells, which is both positive for nuclear and cytoplasm. Furthermore, the number of cells, which is positive only within the nuclear was also counted in 16 images. Based on the number of positive cells, the ratio of cells, which both positive for nuclear and cytoplasm, the ratio of cells, which only positive for nuclear, were calculated. MDV3100 (code 11596, Cayman Chemical, Ann Arbor, MI, United States) was dissolved in DMSO at 10 mM solution. The cell was exposed at 10 μM sokution under the presence of 100 nM DHT.
Mdv3100
Manufactured by Cayman Chemical
Sourced in United States
MDV3100 is a small molecule that acts as an antagonist of the androgen receptor. It is a laboratory research tool used for studying the role of the androgen receptor in biological processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mdv3100
1
Quantifying Nuclear and Cytoplasmic AR Expression
2
Small Molecule Inhibitors Evaluation
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