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Ab100695

Manufactured by Abcam
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Sourced in United Kingdom, Germany

Ab100695 is a monoclonal antibody targeted against the Human Albumin protein. This antibody can be used in various immunoassay applications for the detection and quantification of Human Albumin in biological samples.

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Lab products found in correlation

Ultra sensitive mouse elisa kit, by Crystal Chem (1 mentions) Ezrmcp2 21k, by Merck Group (1 mentions) Eia 1781, by DRG International (1 mentions) Mtr00, by R&D Systems (1 mentions)

4 protocols using ab100695

1

Metabolic Biomarkers in Mice

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In the final week, semi‐fasting blood glucose levels were measured by glucometer on tail blood after 5 h of fasting. After euthanizing, heparinized blood was collected, centrifuged, and plasma obtained for analysis. ELISAs were performed for insulin (Ultra‐Sensitive Mouse ELISA kit, Crystal Chem, Downers Grove, IL) and IGF1 (ab100695, Abcam, Cambridge, UK) according to the manufacturers’ instructions. Triglycerides were determined by a colorimetric assay (BioVision Incorporated, Milpitas, CA).
Li L., Byrd M., Doh K., Dixon P.D., Lee H., Tiwari S, & Ecelbarger C.M. (2016). Absence of renal enlargement in fructose‐fed proximal‐tubule‐select insulin receptor (IR), insulin‐like‐growth factor receptor (IGF1R) double knockout mice. Physiological Reports, 4(23), e13052.
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2

Serum Biomarker Quantification Protocol

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Serum IGF-1 (ab100695, Abcam, Germany), C-peptide (C-peptide 2, EZRMCP2-21K, Millipore, Merck Darmstadt, Germany), and total T4 (EIA 1781, DRG Instruments GmbH, Marburg, Germany) were determined by commercial ELISA kits.
Oelkrug R., Herrmann B., Geissler C., Harder L., Koch C., Lehnert H., Oster H., Kirchner H, & Mittag J. (2017). Dwarfism and insulin resistance in male offspring caused by α1-adrenergic antagonism during pregnancy. Molecular Metabolism, 6(10), 1126-1136.
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3

Maternal Serum IGF1 and Leptin Analysis

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Systemic levels of IGF1 and leptin were assessed in maternal serum using the following kits: Abcam ab100695 and a100718. The procedures were carried out according to the manufacturer’s suggestions. In these analyses, serum from three to four dams of each group was analyzed at E15.
Barbeito-Andrés J., Pezzuto P., Higa L.M., Dias A.A., Vasconcelos J.M., Santos T.M., Ferreira J.C., Ferreira R.O., Dutra F.F., Rossi A.D., Barbosa R.V., Amorim C.K., De Souza M.P., Chimelli L., Aguiar R.S., Gonzalez P.N., Lara F.A., Castro M.C., Molnár Z., Lopes R.T., Bozza M.T., Vianez J.L., Barbeito C.G., Cuervo P., Bellio M., Tanuri A, & Garcez P.P. (2020). Congenital Zika syndrome is associated with maternal protein malnutrition. Science Advances, 6(2), eaaw6284.
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4

Serum Biomarker Quantification in Mice

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Collected mouse sera were stored at –80°C until tested. Sclerostin and IGF1 were measured using ELISA kits for murine Sost (Abcam, ab213889) and murine IGF1 (Abcam, ab100695), and RANKL was measured using an ELISA kit for murine/rat RANKL (R&D Systems, MTR00), according to the manufacturers’ instructions.
Miyagawa K., Tenshin H., Mulcrone P.L., Delgado-Calle J., Subler M.A., Windle J.J., Chirgwin J.M., Roodman G.D, & Kurihara N. (2023). Osteoclast-derived IGF1 induces RANKL production in osteocytes and contributes to pagetic lesion formation. JCI Insight, 8(14), e159838.
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