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Lithocholate

Manufactured by Merck Group
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Lithocholate is a laboratory reagent used in biochemistry and analytical chemistry. Its core function is to serve as a precursor compound in the synthesis and analysis of bile acids and steroid compounds. Lithocholate is a naturally occurring bile acid derivative with a defined molecular structure and purity.

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Lab products found in correlation

Deoxycholate, by Merck Group (2 mentions) Glycocholate, by Merck Group (2 mentions) Taurocholate, by Merck Group (1 mentions) Glycochenodeoxycholic acid, by Merck Group (1 mentions) Tauroursodeoxycholate, by Merck Group (1 mentions) Taurodeoxycholate, by Merck Group (1 mentions) Chenodeoxycholate, by Merck Group (1 mentions) Taurochenodeoxycholate, by Merck Group (1 mentions) Ursodeoxycholate, by Merck Group (1 mentions) Taurolithocholate, by Merck Group (1 mentions) Glycodeoxycholate, by Merck Group (1 mentions) Cholate, by Merck Group (1 mentions) Spexin, by Phoenix Pharmaceuticals (1 mentions) Erythromycin, by Avantor (1 mentions) Sybr green mix, by Quanta Biosciences (1 mentions) Reagent grade dextran sulfate sodium salt dss, by MP Biomedicals (1 mentions) Guaiacol 2 methoxyphenol, by Thermo Fisher Scientific (1 mentions) Mouse recombinant rec lcn2, by Cell Signaling Technology (1 mentions) Qscript cdna synthesis kit, by Quanta Biosciences (1 mentions) Sodium taurocholate, by Merck Group (1 mentions)

2 protocols using lithocholate

1

Bile Acid Standards for Mass Spectrometry

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A series of bile acid standards, containing cholate (CA), chenodeoxycholate (CDCA), ursodeoxycholate (UDCA), deoxycholate (DCA), lithocholate (LCA), glycocholate (GCA), glycochenodeoxycholic acid (GCDCA), glycodeoxycholate (GDCA), taurocholate (TCA), taurodeoxycholate (TDCA), tauroursodeoxycholate (TUDCA), taurohyodexoycholate (THDCA), taurochenodeoxycholate (TCDCA), taurolithocholate (TLCA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tauro-β-muricholate (TβMCA) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). As internal standard (IS), deoxycholic acid-2,2,4,4-d4 (DCA-d4) were obtained from CDN isotopes (Pointe-Claire, Quebec, Canada). All organic reagents for mass spectrometric analysis were HPLC grade purchased from Sigma-Aldrich (St. Louis, MO, USA). And spexin used for drug treatment was purchased from Phoenix Pharmaceuticals (Belmont, CA, USA). M871 was purchased from R&D Systems (Minneapolis, MN, USA). SNAP37889 was purchased from Key Organics Ltd (Camelford, Cornwall, UK).
Lin C.Y., Zhao L., Huang T., Lu L., Khan M., Liu J., Zhong L.L., Cai Z.W., Fan B.M., Wong A.O, & Bian Z.X. (2018). Spexin Acts as Novel Regulator for Bile Acid Synthesis. Frontiers in Physiology, 9, 378.
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2

Detailed Reagent Acquisition for Mouse Colitis Study

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Reagent grade dextran sulfate sodium salt (DSS, M.W. 36–50 kDa, Ref = 160110) was purchased from MP Biomedicals (Solon, OH). Duoset ELISA kits for mouse Lcn2 and keratinocyte-derived chemokine CXCL1 (KC), serum amyloid A (SAA) and biotinylated murine Lcn2 antibody were obtained from R&D Systems (Minneapolis, MN). Hydrogen peroxide (H2O2), myeloperoxidase (MPO), hexadecyl trimethyl ammonium bromide, RNAlater®, TRI Reagent®, iron-free enterobactin (Ent), dimethyl sulfoxide, glucose, sodium taurocholate, glycocholate, lithocholate and deoxycholate were procured from Sigma-Aldrich (St. Louis, MO). Guaiacol (2-methoxyphenol) were obtained from Alfa Aesar (Ward Hill, MA). Erythromycin was purchased from Amresco (Solon, OH). The iron atomic absorption (AA) standard was purchased from RICCA Chemical Company. SYBR® Green mix and qScript cDNA synthesis kit were procured from Quanta Biosciences. Mouse recombinant (rec)-Lcn2 (free from endotoxin, siderophore, and iron) was obtained from Cell Signaling. All other chemicals used in the present study were reagent grade and procured from Sigma.
Saha P., Chassaing B., Yeoh B.S., Viennois E., Xiao X., Kennett M.J., Singh V, & Vijay-Kumar M. (2017). Ectopic Expression of Innate Immune Protein, Lipocalin-2, in Lactococcus lactis Protects against Gut and Environmental Stressors. Inflammatory bowel diseases, 23(7), 1120-1132.
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