Genotyping was initiated by clinicians referring patients in a real-world setting with the intent of clinical use of the results. Sample collection was performed using self-sampling buccal-swab kits. DNA was isolated via MagMax 96 DNA Multi Sample magnetic bead technology with the KingFisher Flex Purification System (Thermo Fisher Scientific, Carlsbad, CA, USA). The genotyping was performed by Genomind Ltd. using standard and custom TaqMan reagents (qPCR) for all variants. CYP2D6 deletions (CYP2D6*5) and duplications were tested by PCR using specific primers.
Magmax 96 dna multi sample
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MagMax 96 DNA Multi Sample is a lab equipment product designed for automated nucleic acid extraction. It utilizes magnetic bead-based technology to efficiently purify DNA samples from a variety of sources in a 96-well format.
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Lab products found in correlation
2 protocols using magmax 96 dna multi sample
1
Genotyping for Clinical Genetics Assessment
2
Multiplex Genotyping of 18 Genes
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Samples were collected using self-sampling buccal-swab kits. DNA was isolated using MagMax 96 DNA Multi Sample magnetic bead technology with the KingFisher Flex Purification System (ThermoFisher Scientific, Carlsbad, CA). The genotyping was performed by Genomind Ltd. using standard and custom TaqMan reagents (qPCR) for all variants. CYP2D6 Copy Number Variation was tested by PCR. The only difference was that the CNV assay did not use a probe, just primers. In total, variants in 18 genes were tested.
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