Log In Sign up
The largest database of trusted experimental protocols

In situ pla probe anti rabbit plus

Manufactured by Merck Group
Visit Supplier
Sourced in United States

The In Situ PLA Probe Anti-Rabbit PLUS is a laboratory equipment product designed for protein detection and visualization. It is a reagent used in the Proximity Ligation Assay (PLA) technique, which allows for the specific and sensitive detection of protein-protein interactions within cells or tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

In situ pla probe anti mouse minus, by Merck Group (2 mentions)

Close spelling variants of this product

Duolink In Situ PLA Probes Anti-rabbit PLUS Duolink anti-Mouse MINUS and anti-Rabbit PLUS In Situ PLA probes PLA probes Anti-rabbit PLUS Rabbit anti-

2 protocols using in situ pla probe anti rabbit plus

1

Proximity Ligation Assay on Rat Ventral Roots

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ventral roots were extracted from adult rats and incubated in neurobasal media with puromycin at 300 µM. Then a fixation with 4% PFA for 1 h was performed and cryosections were made as described above. The PLA protocol was carried out according to the manufacturer's instructions of DuoLink, Sigma using the following reagents: Duolink In Situ PLA Probe Anti-Rabbit PLUS (Cat#DUO92002-30RXN), Duolink In Situ PLA Probe Anti-Mouse MINUS (Cat#DUO92004-30RXN), Duolink In Situ Detection Reagents FarRed (DUO92013-30RXN).
Di Paolo A., Eastman G., Mesquita-Ribeiro R., Farias J., Macklin A., Kislinger T., Colburn N., Munroe D., Sotelo Sosa J.R., Dajas-Bailador F, & Sotelo-Silveira J.R. (2020). PDCD4 regulates axonal growth by translational repression of neurite growth-related genes and is modulated during nerve injury responses. RNA, 26(11), 1637-1653.
+ Open protocol
+ Expand
2

STING-UFL1/TRIM29 Interaction Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The exogenous interaction of STING and UFL1/TRIM29 was detected in HEK293T cells cotransfected with STING-Myc and UFL1-V5/TRIM29-Flag plasmids. PLA was performed with Duolink (Sigma-Aldrich, USA) In Situ PLA technology-related kits, including In Situ Detection Reagents Red (DUO92008), In Situ PLA Probe Anti-Rabbit PLUS (DUO92002) and In Situ PLA Probe Anti-Mouse MINUS (DUO92004). All incubation, ligation and amplification procedures were done according to manufacturer’s instructions.
Tao Y., Yin S., Liu Y., Li C., Chen Y., Han D., Huang J., Xu S., Zou Z, & Yu Y. (2022). UFL1 promotes antiviral immune response by maintaining STING stability independent of UFMylation. Cell Death and Differentiation, 30(1), 16-26.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!