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Potassium hexacyanoferrate 3 244023

Manufactured by Merck Group
Sourced in United States, United Kingdom

Potassium hexacyanoferrate(III) (244023) is a chemical compound used in various laboratory applications. It serves as a source of ferricyanide ions, which can be used in analytical procedures and redox reactions. The product is available in a powder form.

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2 protocols using potassium hexacyanoferrate 3 244023

1

Cellular Signaling Pathway Analysis

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Here, 30% hydrogen peroxide solution (H1009), X-Gal (B4252), potassium hexacyanoferrate(III) (244023), and potassium ferrocyanide (P9387) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bay11-7082 (S2913) was purchased from Selleck Chemicals (Houston, TX, USA). Antibodies against the following proteins were used in this study: Padi2 (66386–1-Ig; Proteintech, Rosemont, IL, USA), α-tubulin (sc-8035; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA), β-actin (sc-47778; Santa Cruz Biotechnology.), LaminA/C (sc-376248; Santa Cruz Biotechnology), p21 (#2947; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse CCL2 antibody (AB-479-NA; R&D Systems, Inc, Minneapolis, MN, USA), mouse CCL5 antibody (AF478; R&D Systems), mouse CCL7 antibody (AF-456-NA; R&D Systems), and normal goat IgG control (AB-108-C; R&D Systems). The following siRNAs used in the present study were purchased from Origene (Rockville, MD, USA): Padi2 siRNA (SR418983B and C), RelA siRNA (SR427138A), Ccl2 siRNA (SR403037C), Ccl5 siRNA (SR400775A), Ccl7 siRNA (SR400957C), and negative control siRNA (SR30004).
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2

Electrochemical Biosensor for Cardiac Troponin I

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The gold microband electrodes and connector (ED-AIO-CELL) were custom fabricated by MicruX Technologies (Spain), the cyclic voltammetry was platform with a EEP-AIO-CELL, and a silver/silver chloride (Ag/AgCl) reference electrode.
Phosphate buffer saline (PBS-D8537), ethanolamine (E0135), potassium hexacyanoferrate (II) trihydrate (455989) and potassium hexacyanoferrate (III) (244023) were purchased from Sigma Aldrich (UK). Cardiac troponin I antibody (PA528964) and cardiac troponin I ELISA kit (ETHNNI3) were purchased from Thermo Fisher Scientific (UK). Recombinant cardiac troponin I protein (ab207624), recombinant Protein G (ab73758) and 3,3'-dithiobis (sulfosuccinimidyl propionate) (DTSSP-ab145616) were purchased from Abcam (UK). Deionized water (Di-H2O) used throughout experiments was maintained at >1 MΩ with a lab deionization unit (Select Analyst, SUEZ Water Technologies & Solutions, France).
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