Multiplex PCR was performed for stool specimen with Seeplex Diarrhea-B2 ACE detection kit (Seegene, Seoul, Korea) to identify the presence of positive E. coli O157 strain for H7 and VTEC (verocytotoxin-producing E. coli) genes. The specimen was cultured with the MacConkey Agar with sorbitol (BD, USA) and picked sorbitol-negative colorless colonies as presumptive E. coli O157:H7 strain for this study. Two probiotic species (Lactococcus lactis and Streptococcus thermophilus) were provided by CTCBio. Inc. Seoul, South Korea.
Sorbitol
Manufactured by BD
Sourced in United States
Sorbitol is a sugar alcohol commonly used as a sweetener and humectant in various laboratory applications. It functions as a cryoprotectant, preservative, and stabilizer in various solutions and samples.
Automatically generated - may contain errors
Lab products found in correlation
Macconkey agar, by BD (1 mentions)
Luminol, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
H2so4, by Merck Group (1 mentions)
Xylenol orange, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by BD (1 mentions)
5 sulphosalicylic acid, by Merck Group (1 mentions)
2 protocols using sorbitol
1
Multiplex PCR for E. coli O157:H7 and VTEC
2
Luminol-Based Chemiluminescence Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Luminol (Sigma-Aldrich, MO, USA), CoCl2 (Univar, IL, USA), Na3PO4 7H2O (Mallinckrodt, Surrey, UK), NaOH (BDH, PA, USA), H2O2 (Chem-Supply Pty Ltd, South Australia, Australia), 5-sulphosalicylic acid (Sigma-Aldrich, MO, USA), ferrous ammonium sulphate (FeSO4(NH4)2SO4.6H2O) (England, UK), H2SO4 (Merck, VIC, Australia), xylenol orange (Sigma-Aldrich, MO, USA), Sorbitol (BDH, PA, USA), 0.45 µM PTFE captiva syringe filters (Agilent, CA, USA). Deionised water purified through a Milli-Q water purification system (Millipore, MA, USA) with a final resistance of 18.2 MΩ was used for all preparations unless mentioned otherwise.
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