Penicillin g

Manufactured by Serva Electrophoresis

Sourced in Germany, United States

Penicillin G is a widely used antibiotic substance produced by certain Penicillium fungi. It is a crystalline compound with the chemical formula C16H18N2O4S.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Streptomycin, by Merck Group (2 mentions) Yeast extract, by Merck Group (1 mentions) Yeast extract, by BD (1 mentions) Bacto agar, by BD (1 mentions) Tryptone, by BD (1 mentions) Egg white lysozyme, by Fujifilm (1 mentions) Peptone, by Merck Group (1 mentions) Ferric citrate, by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Metformin, by Merck Group (1 mentions) Syber green 1 reagent, by Takara Bio (1 mentions) Phenol red free rpmi 1640 with l glutamine, by Thermo Fisher Scientific (1 mentions) Mtt powder, by Merck Group (1 mentions) Silibinin, by Merck Group (1 mentions) First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Penicillin (7 citations)

6 protocols using penicillin g

Spheroplast Formation from D. grandis

Check if the same lab product or an alternative is used in the 5 most similar protocols

A single colony of D. grandis KS 0485 (ATCC 43672) was streaked onto a tryptone glucose yeast extract (TGY) agar plate (5 g/L tryptone [BD, Franklin Lakes, NJ], 1 g/L glucose, 3 g/L yeast extract [BD] and 15 g/L Bacto agar [BD]) and incubated for 2 to 3 d at 30 °C. A single colony was inoculated for primary culture followed by secondary culture in 10 ml of TGY broth. The colony was incubated at 30 °C with shaking, until It reached OD₆₀₀ of 0.7. Cells (6 ml) were harvested via centrifugation at 7,000 rpm (6,684 × g) for 5 min. The supernatant was discarded, and the cells were washed once with 6 mL of PS buffer (4.56 g/L KH₂PO₄, 4.73 g/L Na₂HPO₄, 171 g/L sucrose, pH 7.0) and resuspended in fresh PS buffer. The suspension was incubated with egg white lysozyme (FUJIFILM Wako Pure Chemical, Osaka, Japan) dissolved in PS buffer with 2 mM disodium EDTA (Dojindo, Kumamoto, Japan), at a final concentration of 2 mg/ml. The mixture was incubated at 37 °C for 6 h while shaking gently.
Spheroplasts were centrifuged at 8,000 rpm (4,900 g) for 5 min and resuspended in either MMB0 (5 g/L peptone, 1 g/L yeast extract, 0.1 g/L ferric citrate [Sigma-Aldrich, St. Louis, MO, USA]) containing 300 µg/ml penicillin G [Serva, München] or MMB0 containing penicillin G with different concentrations of CaCl₂[7] (link). penicillin G was added to inhibit regeneration of cell walls in spheroplasts.

Morita Y., Okumura M., Narumi I, & Nishida H. (2019). Sensitivity of Deinococcus grandis rodZ deletion mutant to calcium ions results in enhanced spheroplast size. AIMS Microbiology, 5(2), 176-185.

+ Open protocol

+ Expand

Bacterial L-forms and Spheroplast Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bacterial L-forms and protoplasts (or spheroplasts) lack a cell wall. Although protoplasts do not normally undergo prolonged growth or division, L-forms divide via various processes, including membrane blebbing, tubulation, vesiculation, and fission (Errington, 2013) . Spheroplasts or L-forms can recover to their native forms and cell wall synthesis plays an important role in this (Billings et al., 2014; Kawai et al., 2014; Ranjit and Young, 2013) . For example, spheroplasts lacking penicillin binding protein 1B (related to the synthesis of cross-linked peptidoglycan) cannot divide or recover their native forms (Ranjit and Young, 2013) . Interestingly, incubation of the spheroplasts formed by lysozymes in the presence of peni- The delta Cq-value is the Cq value at 0 h of growth minus the Cq value at each subsequent time point (Table 1). for 5 min at 3000 rpm) and resuspended in marine broth (500 µL) containing 600 µg/mL penicillin G (Serva). This suspension (2 µL) was diluted into marine broth (500 µL) containing 600 µg/mL penicillin G. The suspension was incubated at 24°C. We used spheroplasts from various time points of growth: 0 h, 3 h, 6 h, 9 h, 12 h, and 24 h. Three biological replicates were collected for each sample.

Takahashi S, & Nishida H. (2015). Quantitative analysis of chromosomal and plasmid DNA during the growth of spheroplasts of Escherichia coli. The Journal of general and applied microbiology, 61(6).

+ Open protocol

+ Expand

MCF-7 Breast Cancer Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

2.9.1. Cell culture.
An MCF-7 human breast cancer cell line (VACSERA, Egypt) was cultured in an RPMI1640 medium (Gibco, Invitrogen, Carlsbad, CA) supplemented with 10% heatinactivated fetal bovine serum (FBS) (Gibco, Invitrogen), 2 g/mL sodium bicarbonate, 80 mg/mL penicillin G (Serva, Germany), 50 mg/mL streptomycin (Merck, Germany), and incubated at 37°C with humidified air containing 5% CO2.

Taleb S.A., Sobh R., & Mourad R.M. (2021). Investigating the Effect of Loading Curcuminoids Using PCL-PU-βCD Nano-Composites on Physico-Chemical Properties, In-Vitro Release, and Ex-Vivo Breast Cancer Cell-Line. Biointerface Research in Applied Chemistry, 12(3), 4074-4102.

+ Open protocol

+ Expand

Breast Cancer Cell Line Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Silibinin, Metformin and MTT powder were obtained from Sigma (Deisenhofen, Germany); T47D breast cancer cell line purchased from the Pasteur Institute of Iran, Tehran, Iran; fetal bovine serum (FBS) and phenol-red free RPMI 1640 with L glutamine were purchased from Gibco BRL (Life Technologies, Grand Island, NY, USA); Sodium bicarbonate and Streptomycin were purchased from Merck (Darmstadt, Germany); Penicillin G was purchased from SERVA (Heidelberg, Germany); TRIZOL reagent was purchased from Invitrogen (Eugene, OR, USA); First-Strand cDNA Synthesis kit was purchased from Fermentas (Hanover, MD, USA); and Syber Green-I reagent was purchased from Takara Bio (Otsu, Japan).

Chatran M., Pilehvar-Soltanahmadi Y., Dadashpour M., Faramarzi L., Rasouli S., Jafari-Gharabaghlou D., Asbaghi N, & Zarghami N. (2018). Synergistic Anti-proliferative Effects of Metformin and Silibinin Combination on T47D Breast Cancer Cells via hTERT and Cyclin D1 Inhibition. Drug research, 68(12).

+ Open protocol

+ Expand

Preparation and Incubation of Giant Spheroplasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Giant spheroplasts were prepared using a previously described method with modifications (Kusaka, 1967) . Spheroplast incubation was performed according to the method reported by Kuroda et al. (1998) . Cells of the Erythrobacter litoralis NBRC 102620 strain were grown in marine broth agar (Difco Co.) under aerobic conditions. The harvested cells (0.003 g) were suspended in buffer (1 mL) consisting of 0.1 M Tris-HCl (pH 7.4) and 0.3 M sucrose. Lysozyme (Wako Co.) (200 µg/mL) was added to the cell suspension, which was then incubated at 25°C for 15 min. Afterwards, the suspension was divided into 2 aliquots (500 µL each), followed by harvesting (centrifugation for 5 min at 3000 rpm) and resuspension in marine broth (500 µL) containing 600 µg/mL penicillin G (Serva). The sus-

Takayanagi A., Takahashi S, & Nishida H. (2016). Requirement of dark culture condition for enlargement of spheroplasts of the aerobic anoxygenic photosynthetic marine bacterium Erythrobacter litoralis. The Journal of general and applied microbiology, 62(1).

+ Open protocol

+ Expand

Spheroplast Cultivation of E. litoralis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cultivation of E. litoralis spheroplasts. Cells of E. litoralis NBRC 102620 were grown on marine broth agar (Difco, Detroit, MI, USA). The harvested cells (approximately 3 mg) were suspended in a buffer (1 mL) consisting of 0.1 M Tris-HCl (pH 7.4) and 0.3 M sucrose. Lysozyme (Wako Co., Osaka, Japan) (200 mg/mL) was added to the cell suspension, which was then incubated at 25∞C for 15 min. After centrifugation for 5 min at 3000 rpm, the cells were suspended in marine broth (1 mL) containing 600 mg/mL penicillin G (Serva, Heidelberg, Germany). The suspension was then diluted by adding 160 mL of the suspension to marine broth (80 mL) containing penicillin G. The spheroplasts were incubated at 25∞C under aero-bic and anaerobic light-dark (12 h each) conditions. BMS-PS08RGB3 (Bio Medical Science, Tokyo, Japan) was used for the light conditions. Two different lights, blue (400-500 nm) and red (600-700 nm), were used. The spheroplasts were cultured for 36 h (light-dark-light conditions, each 12 h). Blue and red light intensities were 25 mmol/m 2 /s and 27 mmol/m 2 /s, respectively. The cells were sampled immediately at 36 h of growth. BIONIX2 (Sugiyama-Gen, Tokyo, Japan) was used for culture under oxygen-free conditions. The culture dish was placed in a plastic bag, and a capsule of deoxidizing agent was opened and placed in the plastic bag with an oxygen meter.

Nishino K., Takahashi S, & Nishida H. (2018). Comparison of gene expression levels of appA, ppsR, and EL368 in Erythrobacter litoralis spheroplasts under aerobic and anaerobic conditions, and under blue light, red light, and dark conditions. The Journal of general and applied microbiology, 64(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!