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Matchmaker tm insert check pcr mix

Manufactured by Takara Bio
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Sourced in Japan

The Matchmaker TM Insert Check PCR Mix is a reagent used to verify the presence of an insert in a cloned vector. It contains the necessary components for a polymerase chain reaction (PCR) to amplify the insert region, allowing users to confirm the successful cloning of their desired genetic sequence.

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Lab products found in correlation

Sd leu aba aureobasidin a, by Takara Bio (2 mentions) Yeastmaker yeast transformation system 2, by Takara Bio (2 mentions)

2 protocols using matchmaker tm insert check pcr mix

1

Yeast One-Hybrid Assay for SlBES1.8 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
The promoter fragments of SlGA2ox2, SlGA2ox6 and SlGID1b-1 were constructed into pAbAi plasmid and transformed into Y1HGold yeast strain under the guidance of Yeastmaker™ Yeast Transformation System 2 (TAKARA, Japan). The recombinant yeast strain was confirmed by PCR conducted with Matchmaker TM Insert Check PCR Mix (TAKARA, Japan). The full length of SlBES1.8 coding sequence was cloned into pGADT7 plasmid and subsequently transformed into the recombinant yeast strain.
The empty pGADT7 plasmid was used as negative control. The transformants were cultivated on SD/-Leu or SD/-Leu/AbA (Aureobasidin A, Clontech, USA) medium for three days. DNA-protein interactions between SlBES1.8 and promoter fragments were determined by the growth of colony.
Su D., Xiang W., Liang Q., Wen L., Shi Y., Song B., Liu Y., Xian Z, & Li Z. (2022). Tomato SlBES1.8 Influences Leaf Morphogenesis by Mediating Gibberellin Metabolism and Signaling. Plant & cell physiology, 63(4).
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2

Yeast One-Hybrid Assay for SlBES1.8 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
The promoter fragments of SlGA2ox2, SlGA2ox6 and SlGID1b-1 were constructed into pAbAi plasmid and transformed into Y1HGold yeast strain under the guidance of Yeastmaker™ Yeast Transformation System 2 (TAKARA, Japan). The recombinant yeast strain was confirmed by PCR conducted with Matchmaker TM Insert Check PCR Mix (TAKARA, Japan). The full length of SlBES1.8 coding sequence was cloned into pGADT7 plasmid and subsequently transformed into the recombinant yeast strain.
The empty pGADT7 plasmid was used as negative control. The transformants were cultivated on SD/-Leu or SD/-Leu/AbA (Aureobasidin A, Clontech, USA) medium for three days. DNA-protein interactions between SlBES1.8 and promoter fragments were determined by the growth of colony.
Su D., Wei X., Qin L., Wen L., Shi Y., Liu Y., Xian Z., & Li Z. (2021). Tomato SlBES1.8 influences leaf morphogenesis by mediating gibberellin metabolism and signaling.
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