Cisplatin

The JAK1/2 inhibitor ruxolitinib was purchased from Calbiochem, part of Merckm Darmstadt, Germany) and used at a final concentration of 10 μM unless otherwise stated. The JAK2 inhibitor fedratinib was a kind gift from Dr. Edwin Chen (University of Leeds, Leeds, UK) and used at a final concentration of 10 μM unless otherwise stated. The antipsychotic drug pimozide was purchased from Calbiochem and used at a final concentration of 10 μM unless otherwise stated. Cisplatin was purchased from Cambridge Bioscience and used at a concentration of 2 μM, unless otherwise stated.

Anti-B7-H3 (ThermoFisher, Loughborough, UK (IP), AF1027 BD Bioscience, Wokingham, UK (WB), and MAB1027 Santa Cruz, Heidelberg, Germany (IF)), anti-EEA1 (Cell Signaling, Leiden, The Netherlands), anti-HSC70 (Santa Cruz), anti-IMPDH2 (Proteintech), anti-Rab10 (Abcam, Cambridge, UK), anti-Rab11 (Cell Signaling), and HTII-280 AT2 (terrace Biotech, San Francisco, USA) were used. IgG negative control was used for IP and obtained from DAKO. DAPI (Sigma-Aldrich, Gillingham, UK) was used as nuclear stain for immunofluorescence. LysoTracker™ Deep Red was used to stain lysosomes (ThermoFisher). Anti-mouse-HRP, anti-rabbit-HRP, and anti-goat-HRP were from DAKO; anti-mouse-568, anti-rabbit-568, anti-rabbbit-488, and phalloidin-488 and -647 were all obtained from Invitrogen. Other reagents used were CellROX™ Deep Red Reagent (for oxidative stress detection) (ThermoFisher). CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher), Cisplatin (Cambridge Bioscience, Cambridge, UK), and Mycophenolic acid (MPA; IMPDH inhibitor) (Sigma-Aldrich).