The clinical characteristics of patients were obtained from the electronic medical records: age, sex, type of infection, comorbidities, haematological parameters and liver function tests. The complete blood count parameters including red cell count (RBC), white cell count (WBC) and platelet (PLT) count were analysed by the impedance method on a Sysmex XN-2000 hematology analyzer (Sysmex Corporation, Kobe, Japan). The blood liver function parameters including serum gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin values were measured by the dry-chemistry method on a VITROS 5600 automated chemistry analyzer (Ortho Clinical Diagnostics, Raritan, NJ). They were used for the calculation of inflammatory parameters. The PLR was defined as PLT to lymphocyte ratio; NLR was defined as neutrophil to lymphocyte ratio; GPR was defined as GGT to PLT ratio; APPR was defined as ALP to PLT ratio; ALPR was defined as ALT to PLT ratio; APRI was defined as AST to PLT ratio; PNI was calculated as serum albumin level (g/L)+5 × total lymphocyte count (×109/L). Postoperative haematological parameters within a month after the operation were also collected for analyses in all patients.
Xn 2000 hematology analyzer
Manufactured by Sysmex
Sourced in Japan
The XN-2000 Hematology Analyzer is a laboratory instrument manufactured by Sysmex. It is designed to perform complete blood count (CBC) and other hematology-related tests. The XN-2000 utilizes advanced technology to analyze various blood parameters, providing comprehensive data to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
Vitros 5600 automated chemistry analyzer, by Ortho Clinical Diagnostics (2 mentions)
Paxgene blood rna tube, by BD (1 mentions)
F4 80 fitc, by BioLegend (1 mentions)
Cd11b apc, by BioLegend (1 mentions)
Gr 1 fitc, by BioLegend (1 mentions)
Cd115 pe, by BioLegend (1 mentions)
Cd19 percp, by BioLegend (1 mentions)
Kaluza software, by Beckman Coulter (1 mentions)
Xn 1000, by Sysmex (1 mentions)
Cd11b, by BD (1 mentions)
Facscanto, by BD (1 mentions)
F4 80, by BD (1 mentions)
Cd45 microbeads, by Miltenyi Biotec (1 mentions)
Au5800 biochemical analyzer, by Beckman Coulter (1 mentions)
7180 automatic biochemical analyzer, by Hitachi (1 mentions)
Au5800 chemistry analyzer, by Beckman Coulter (1 mentions)
12 protocols using xn 2000 hematology analyzer
1
Hematological Biomarkers in Infection
2
Blood Sample Analysis for Exercise Recovery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were taken right before (Pre), right after the exercise (Post), and then 1 (Post1), 2 (Post2), and 7 days (Post7) later to follow restitution. Blood collection was performed by a professional healthcare nurse. Briefly, after skin disinfection, BD Vacutainer blood drawing system was used. Plastic EDTA tubes (BD Vacutainer, Cat. No.: 367863) were used for total blood count, plastic serum, and glass plasma tubes for additional examinations (BD Vacutainer, Cat. No.: 367815, 369714), and PAXgene Blood RNA tubes for whole blood RNA isolation (BD Biosciences, Cat. No.: 762165). The total volume was ∼15 mL for each blood drawing. The qualitative cell counts in the blood samples were determined using a Sysmex XN-2000 hematology analyzer (Sysmex Corporation, Kobe, Japan). Routine laboratory parameters in serum and urine samples were measured at the Department of Laboratory Medicine, University of Debrecen.
3
Hematological Biomarkers in Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The clinical characteristics of patients were obtained from the electronic medical records: age, sex, type of infection, comorbidities, haematological parameters and liver function tests. The complete blood count parameters including red cell count (RBC), white cell count (WBC) and platelet (PLT) count were analysed by the impedance method on a Sysmex XN-2000 hematology analyzer (Sysmex Corporation, Kobe, Japan). The blood liver function parameters including serum gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin values were measured by the dry-chemistry method on a VITROS 5600 automated chemistry analyzer (Ortho Clinical Diagnostics, Raritan, NJ). They were used for the calculation of inflammatory parameters. The PLR was defined as PLT to lymphocyte ratio; NLR was defined as neutrophil to lymphocyte ratio; GPR was defined as GGT to PLT ratio; APPR was defined as ALP to PLT ratio; ALPR was defined as ALT to PLT ratio; APRI was defined as AST to PLT ratio; PNI was calculated as serum albumin level (g/L)+5 × total lymphocyte count (×109/L). Postoperative haematological parameters within a month after the operation were also collected for analyses in all patients.
4
RA Patients' MTX Response and RDW
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unit of the Maggiore della Carità Hospital in Novara between 1 st January 2010 and 31 st December 2018. We included patients who: 1) fulfilled 2010 ACR/Eular classification Criteria (11); 2) were not on MTX and were starting MTX; 3) had a chemistry panel performed at the central laboratory of the Maggiore della Carità Hospital in Novara, at the beginning of MTX treatment and after 3 months. Concomitant treatments with hydroxychloroquine or low dose prednisone were admitted.
The study has been conducted in accordance with the local ethical guidelines. Out of 233 records, 45 were discarded because they presented overlapping characteristics with other rheumatologic diseases and 106 because of the unavailability of laboratory data at baseline and/or after 3 months of MTX treatment. Thus, the final study population included 82 RA patients. We recorded demographic, clinical and laboratory data at baseline and after treat-ment. RDW was measured by XN2000 Hematology Analyzer, Sysmex. Treatment response was evaluated at the follow-up visit at 3-months and graded according to EULAR response criteria (12).
The study has been conducted in accordance with the local ethical guidelines. Out of 233 records, 45 were discarded because they presented overlapping characteristics with other rheumatologic diseases and 106 because of the unavailability of laboratory data at baseline and/or after 3 months of MTX treatment. Thus, the final study population included 82 RA patients. We recorded demographic, clinical and laboratory data at baseline and after treat-ment. RDW was measured by XN2000 Hematology Analyzer, Sysmex. Treatment response was evaluated at the follow-up visit at 3-months and graded according to EULAR response criteria (12).
5
Comprehensive Blood Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After getting written consent from each participant, five milliliters of blood was collected in an EDTA containing vacutainer tube with the help of expert phlebotomists. Blood was transferred to the laboratory of population genetics using a sample carrier. Complete Blood Count (CBC) analyses were performed using the Sysmex XN-2000 Hematology Analyzer that uses the direct current sheath flow detection method. From CBC analyses RBC Count, hematocrit level, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), RBC distribution width (RDW), total WBC count, differential count including percentages of neutrophils, lymphocytes, monocytes, eosinophils, basophils and eosinophils were determined.
6
Immunophenotyping of Bone Marrow Myeloid Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bone marrow myeloid cells were obtained by flushing tibiae and femurs with growth medium, and enriched for CD45 expression using CD45 MicroBeads (Miltenyi Biotec) and magnetic-activated cell sorting according to the manufacturer’s instructions. Cells were spun down and labelled with the following antibodies for 45 min at 4 °C: APC-CD11b (101211, BioLegend), FITC-Gr-1 (108405, BioLegend), FITC-F4/80 (123107, BioLegend), PE-CD115 (165203, BioLegend) and PerCP-CD19 (115531, BioLegend). The number of Gr-1+CD11b+ neutrophils, F4/80+CD11b+ macrophages, CD115+CD11b+ monocytes and CD19+ B cells was assessed by flow cytometry (BD FACSCanto, BD Biosciences) as described before49 (link) and quantified using Kaluza software (Beckman Coulter).
Circulating myeloid cells in blood were analysed using the Sysmex XN1000 and XN2000 Hematology Analyzer (Sysmex Corporation; University Hospital Leuven).
Circulating myeloid cells in blood were analysed using the Sysmex XN1000 and XN2000 Hematology Analyzer (Sysmex Corporation; University Hospital Leuven).
7
Pleural Fluid Biomarker Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A pleural fluid specimen was collected into an anticoagulant‐free tube at the time of patient admission. After centrifugation, the supernatant of the pleural fluid was collected and stored at −70°C until analysis. In both cohorts, homocysteine was measured by a Beckman AU5800 biochemical analyzer in November 2021. The coefficient variations (CVs) of homocysteine were 2.83% and 8.92% at concentrations of 23.35 and 5.53 μmol/L, respectively. The laboratory technician who measured the homocysteine level did not know the patients' clinical details.
Pleural fluid concentrations of glucose, adenosine deaminase (ADA), white blood cells (WBCs), lactate dehydrogenase (LDH), and protein were collected from the medical records of the participants. Pleural glucose was determined by the hexokinase method. LDH was determined by the lactate‐to‐pyruvate method. ADA was determined by the peroxidase method.21 Total protein was determined by the biuret method. LDH, ADA, and glucose were determined by a Beckman AU5800 biochemical analyzer. WBC counts in the pleural fluid were determined by a Sysmex XN 2000 hematology analyzer.
Pleural fluid concentrations of glucose, adenosine deaminase (ADA), white blood cells (WBCs), lactate dehydrogenase (LDH), and protein were collected from the medical records of the participants. Pleural glucose was determined by the hexokinase method. LDH was determined by the lactate‐to‐pyruvate method. ADA was determined by the peroxidase method.
8
Routine Blood Test Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using freshly collected fasting peripheral blood samples, we performed routine biochemical variable testing. Lipid parameters were tested by the Dimension AR/AVL Clinical Chemistry System (DADE Bchring, Newark, NJ). Platelet parameters were examined by the CL TOP coagulation analyzer (Instrumentation Laboratory, USA). Hemocyte parameters were detected by the XN-2000 hematology analyzer (SYSMEX, Japan).
9
Serum IgA Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples (5 mL) were collected into Vacutainer tubes anticoagulated with ethylenediaminetetraacetic acid (EDTA) (Vacutainer Systems, Rutherford, NJ, USA) and serum IgA levels were detected using Sysmex XN-2000 Hematology Analyzer (Sysmex Hungary, Budapest, Hungary).
10
Biomarkers in Revision Arthroplasty
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples for detecting ESR and coagulation-related indicators were collected as part of the routine examination after admission. Then all were sent to the medical laboratory for detection as soon as possible. The ESR was detected using Westergren methods, and coagulation was quantified with a Sysmex CA-500 automatic blood coagulation analyzer (Sysmex, Japan). Synovial fluid was extracted before opening the joint capsule during revision surgery and then using a Sysmex XN2000 hematology analyzer (Sysmex, Japan) to determine synovial fluid (SF) white blood cell (WBC) count and the polymorphonuclear neutrophil percentage (PMN%). For D-lactate, one milliliter of blood and synovial fluid were collected from each sample above. After centrifugation, the supernatant was collected and stored in a refrigerator at -80°C until the final tests could be conducted uniformly.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!