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Ha 11 monoclonal antibody

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The HA.11 monoclonal antibody is a laboratory reagent used for research purposes. It is a mouse-derived antibody that specifically recognizes the influenza A virus hemagglutinin (HA) protein. The HA.11 antibody can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA), to detect and study the HA protein.

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Lab products found in correlation

Chicken serum, by Thermo Fisher Scientific (1 mentions) Hemin, by Merck Group (1 mentions) Primerscript first strand cdna synthesis kit, by Takara Bio (1 mentions) Pfu ultra 2 hotstart 2x master mix, by Agilent Technologies (1 mentions) Dylight 800cw secondary antibodies, by Thermo Fisher Scientific (1 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (1 mentions) β mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Anti flag m2 monoclonal antibody, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Kh2po4, by Merck Group (1 mentions) Folic acid, by Merck Group (1 mentions) Na2hpo4, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Restriction enzyme, by New England Biolabs (1 mentions)

4 protocols using ha 11 monoclonal antibody

1

Comprehensive Antibody and Construct Protocols

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Commercial antibodies to SHIP2, Mena, and VASP (rabbit) were obtained from Cell Signaling Technology; SHIP2 and VASP (mouse) from Santa Cruz Biotechnology, Inc.; vinculin, actin, and tubulin from Sigma-Aldrich; GFP antibody, phalloidin Alexa Fluor 546, and Alexa Fluor 488– and 555–conjugated secondary antibodies from Thermo Fisher Scientific; and HA.11 monoclonal antibody from Covance. HA-SHIP2 and GFP-SHIP2 were gifts from C.A. Mitchell (Monash University, Clayton, Australia) and K. Kaibuchi (Nagoya University, Nagoya, Japan), respectively. GFP-VASP and GFP-Mena were gifts from F. Gertler (Massachusetts Institute of Technology, Cambridge, MA). pLifeAct-TagRFP was purchased from Ibidi. Point mutants of pEGFP-N3-SHIP2 were generated by site-directed mutagenesis using a kit from Stratagene (Agilent Technologies). Deletion mutants of pEGFP-N3-SHIP2, GFP-VASP, and GFP-Mena were generated by PCR amplification. mCherry-Mena and pLXSN-SHIP2 WT and mutants were generated by subcloning. Mitochondria targeting vector (MITO-GFP) was generated by subcloning the presequence of Su9 from ATPase subunit 9 of N. crassa into pGFP-C2 vector (Frederick et al., 2008 (link)). MITO-SHIP2 was generated by PCR amplification of SHIP2 and insertion into the EcoRI and XhoI sites of the MITO-GFP vector. SHIP2 deletion mutants lacking aa 18–108 (SHIP2ΔSH2) and 1,048–1,059 (SHIP2ΔFP4) were generated by PCR.
Rajadurai C.V., Havrylov S., Coelho P.P., Ratcliffe C.D., Zaoui K., Huang B.H., Monast A., Chughtai N., Sangwan V., Gertler F.B., Siegel P.M, & Park M. (2016). 5′-Inositol phosphatase SHIP2 recruits Mena to stabilize invadopodia for cancer cell invasion. The Journal of Cell Biology, 214(6), 719-734.
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2

Detecting HA-tagged TREM2 protein

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The HA.11 monoclonal antibody from Covance was used to detect HA-tagged TREM2. The transferrin receptor (TfR) monoclonal antibody was from Invitrogen.
Bonham L.W., Sirkis D.W., Fan J., Aparicio R.E., Tse M., Ramos E.M., Wang Q., Coppola G., Rosen H.J., Miller B.L, & Yokoyama J.S. (2017). Identification of a rare coding variant in TREM2 in a Chinese individual with Alzheimer’s disease. Neurocase, 23(1), 65-69.
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3

HA-TREM2 Detection with Antibodies

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The HA.11 monoclonal antibody used to detect HA-TREM2 was from Covance, and the clathrin heavy chain monoclonal antibody was from BD Transduction Laboratories.
Sirkis D.W., Bonham L.W., Aparicio R.E., Geier E.G., Ramos E.M., Wang Q., Karydas A., Miller Z.A., Miller B.L., Coppola G, & Yokoyama J.S. (2016). Rare TREM2 variants associated with Alzheimer’s disease display reduced cell surface expression. Acta Neuropathologica Communications, 4(1), 98.
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4

Molecular Cloning Protocol Compendium

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Restriction enzymes were from New England Biolabs. Pfu Ultra II Hotstart 2X Master Mix was from Agilent. PrimerScript First Strand cDNA synthesis kit was from TaKaRa. Clone ID 1X Colony PCR Master Mix was from Lucigen. Peptone was obtained from Bacto. Yeast extract was from BD Transduction laboratories. KH2PO4, Na2HPO4, yeast nucleic acid, folic acid, hemin and Monoclonal anti-Flag M2 antibody were from Sigma. HA.11 monoclonal antibody was purchased from Covance. Fetal bovine serum was from Gemini Bio-products. T4 ligase and Dylight™ 700CW and Dylight™800CW secondary antibodies were from Thermo Scientific. Protein A/G Plus-agarose was from Santa Cruz Biotechnology. DMEM (Dulbecco’s modified Eagle medium), RPMI 1640 media, chicken serum, beta mercaptoethanol and G418 sulfate and Trizol were from Invitrogen. Reagents used for SDS-PAGE were purchased from Bio-Rad.
Alzayady K.J., Sebé-Pedrós A., Chandrasekhar R., Wang L., Ruiz-Trillo I, & Yule D.I. (2015). Tracing the Evolutionary History of Inositol, 1, 4, 5-Trisphosphate Receptor: Insights from Analyses of Capsaspora owczarzaki Ca2+ Release Channel Orthologs. Molecular Biology and Evolution, 32(9), 2236-2253.
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