Tdt mediated dutp nick end labeling tunel kit

Apoptosis was measured via a TdT-mediated dUTP Nick-End Labeling (TUNEL) kit (KeyGen, Jiangsu, China). Briefly, the treated cells were fixed with 4% paraformaldehyde (KeyGen, Jiangsu, China) for 30 min and permeabilized for 5 min. TDT enzyme reaction solution (KeyGen, Jiangsu, China) was added to each well, and reactions were incubated in the dark at room temperature for 1 h. The cells were mixed with streptavidin labeling solution (KeyGen, Jiangsu, China) and incubated in the dark for 30 min. DAPI was added to counterstain the nuclei after washing the cells three times with PBS. After staining, the percentage of TUNEL-positive cells was counted.

Na¹³¹I was purchased from Shanghai XinKe Pharmaceutical Co., Ltd. ²²³RaCl₂ was purchased from the Institute for Energy Technology. The operation related to the above agents was performed in a hot cell. Ethiodized poppyseed oil injection was purchased from Jiangsu HengRui Medicine Co., Ltd. Silk fibroin microspheres (about 150.61 ± 10.39 μm in diameter, controlled by steel screens) were purchased from Suzhou Meilun Biotechnology Co., Ltd. The TdT-mediated dUTP Nick-End Labeling (TUNEL) kit was purchased from KeyGEN Biotechnology Co., Ltd. The annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis kit was purchased from BD Bioscience. A cell counting kit-8 (CCK-8) was purchased from the Beyotime Institute of Biotechnology. A γ-H2AX ELISA kit was purchased from Jiangsu Meimian Industrial Co., Ltd.
A human hepatocyte carcinoma cell line (Huh-7) was purchased from the Cell Bank of the Type Culture Collection of the Chinese Academy of Sciences, Shanghai Institute of Cell Biology, Chinese Academy of Sciences. The New Zealand white rabbits (male, weighing 2.5 ± 0.5 kg) were purchased from Ailingfei Biotechnology Co., Ltd., and kept under a specific pathogen-free condition at the laboratory animal center. This research was approved and guided by the Ethics Committee of Naval Medical University (Approval No. 1207050662).