Bz 8000

Manufactured by Keyence

Sourced in Japan, Germany, United States

The BZ-8000 is a high-performance fluorescence microscope designed for advanced biological and material science applications. It offers high-resolution imaging, powerful analysis features, and a user-friendly interface. The BZ-8000 is capable of capturing clear and detailed images of a wide range of samples, including cells, tissues, and materials. Its core function is to provide advanced imaging and analysis capabilities to researchers and scientists.

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Lab products found in correlation

Matrigel, by Corning (9 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (9 mentions) Trap alp stain kit, by Fujifilm (3 mentions) Hoechst 33342, by Merck Group (3 mentions) Bz 9000, by Keyence (3 mentions) Prolong gold, by Thermo Fisher Scientific (3 mentions) Nonspecific staining blocking reagent, by Agilent Technologies (2 mentions) Cm 1510s, by Leica (2 mentions) Bz x analyzer, by Keyence (2 mentions) Hoechest dye hoechst 33342, by Lonza (2 mentions) Bz analyzer, by Keyence (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions) Glycerol, by Merck Group (2 mentions) Cytospin 4 centrifuge, by Thermo Fisher Scientific (2 mentions) Cm1510, by Leica (2 mentions) Bz x800, by Keyence (2 mentions) Oil red o, by Merck Group (2 mentions) Bz x710, by Keyence (2 mentions) 6 well plate, by BD (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Biozero BZ-8000 (70 citations) BZ-8000 microscope (45 citations) BZ-8000 fluorescence microscope (25 citations) Biozero BZ-8000 microscope (13 citations) BZ-8000 confocal microscope (8 citations) BZ-8000 digital microscope (4 citations) Biozero BZ-8000 fluorescence microscope (3 citations) BZ-8000 fluorescent microscope (2 citations)

164 protocols using bz 8000

In Vitro and In Vivo TPFC Fluorescence Microscopy

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For the in vitro fluorescence microscopy study, F98 rat glioma cells were seeded in a twowell chamber mounted on glass slides with a cover (Thermo Fisher Scientific Inc., Rochester, NY), and the culture medium without TPFC was exchanged for TPFC-containing culture medium just before confluence. The cells were exposed to 20 μg 10 B/mL of TPFC for 24 h. After exposure, the glass slides were washed with 4°C PBS and the two-well chamber was removed. The nuclei specific Hoechest dye (Hoechst 33342; Lonza) was added (10 μg/mL) and the glass slides mounted onto cover glasses using DPX Mountant for histology (Fluka Biochemika 44581, Germany). The two-well chamber slides were observed using an inverted fluorescence microscope system (BZ-8000; KEYENCE, Japan).
The in vivo fluorescence microscopy study was conducted twenty four hours after termination of CED administration of TPFC to F98 rat glioma-bearing brain tumor model. The rats were euthanized and strip preparations of the brains were made. Similar to the in vitro fluorescence microscopy study, the strip preparations of brain were observed using an inverted fluorescence microscope system (BZ-8000; KEYENCE).

Hiramatsu R., Kawabata S., Tanaka H., Sakurai Y., Suzuki M., Ono K., Miyatake S.I., Kuroiwa T., Hao E, & Vicente M.G.H. (2015). Tetrakis(p-Carboranylthio-Tetrafluorophenyl)Chlorin (TPFC): Application for Photodynamic Therapy and Boron Neutron Capture Therapy. Journal of pharmaceutical sciences, 104(3).

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Detecting SIRT1 in Oocytes

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Immature and mature oocytes were denuded from granulosa cells, and SIRT1 in oocytes was detected as described previously [24] (link). The primary and secondary antibodies used for this procedure were rabbit polyclonal anti-SIRT1 (1∶500; Santa Cruz Biotechnology, Santa Cruz, CA) and fluorescein-conjugated goat anti-rabbit IgG (1∶1000; Cell Signaling Technology Inc., Beverly, MA), respectively. The oocytes were mounted on glass slides using an antifade reagent containing DAPI (ProLong gold antifade reagent with DAPI; Invitrogen, OR, USA), and were observed using a fluorescence digital microscope (BZ-8000; Keyence, Tokyo, Japan). Fluorescence images of the oocyte were captured, and the fluorescence intensity was measured using the ImageJ software (BZ-8000; Keyence, Tokyo, Japan). To validate the immunostaining, the oocytes were cultured with the primary antibody (2 µg/mL IgG) or primary antibody and SIRT1 peptide (Abcam 7770-100, 2 or 10 µg/mL). As expected, the fluorescence intensity decreased significantly in a peptide concentration-dependent manner.

Sato D., Itami N., Tasaki H., Takeo S., Kuwayama T, & Iwata H. (2014). Relationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine Oocytes. PLoS ONE, 9(4), e94488.

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Oocyte Maturation Stage Analysis

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After IVG, oocytes were denuded from OGCs and fixed in PBS containing 4% paraformaldehyde. Oocytes were stained with Hoechst 33342 (1 µg/mL, Sigma) for 15 minutes and observed under a fluorescence microscope (BZ‐8000; Keyence). According to a previous report,3 chromatin configuration was subdivided into five categories, and the prominent features of full‐grown oocytes at germinal vesicle stages were categorized as GV1 (oocytes having a nuclear membrane and intact but condensed chromatin forming a ring or horseshoe around the nucleolus). In the present study, the percentage of oocytes at the stage GV1, considered as full‐grown oocytes, was determined under a digital microscope (BZ‐8000; Keyence).

Shibahara H., Ishiguro A., Shirasuna K., Kuwayama T, & Iwata H. (2019). Follicular factors determining the developmental competence of porcine oocyte. Reproductive Medicine and Biology, 18(3), 256-262.

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Fluorescent Protein Imaging and Live-Cell Chromosome Dynamics

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For the analysis of fluorescently tagged proteins, cells were cultured on glass coverslips, washed once with PBS, and fixed with 4% paraformaldehyde in PBS for 10 min. For the observation of the spindle microtubules, cells were permeabilized with 0.2% Triton X-100 for 5 min, blocked for 30 min using blocking buffer (1% BSA), and stained with an anti-Tubulin (ab7291, Abcam) monoclonal antibody. The nuclei DNA were counterstained by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Fluorescent images were captured by using either a Biozero microscope (BZ-8000, Keyence) or a confocal microscopy (FV1000, Olympus).
For chromosome misalignment and missegregation analysis, live cells expressing EGFP-H4 were grown on the glass bottom dishes and chromosome movements were recorded according to the green signals with an interval of 5 min by using a Biozero microscope (BZ-8000, Keyence) system. All the images were processed in ImageJ.

Li Z., Cui Q., Xu J., Cheng D., Wang X., Li B., Lee J.M., Xia Q., Kusakabe T, & Zhao P. (2017). SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori. Scientific Reports, 7, 15536.

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In vitro and in vivo Fluorescence Microscopy of Boron-containing Compounds

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For the in vitro fluorescence microscopy study, F98 rat glioma cells were seeded in a two-well chamber mounted on glass slides with a cover (Thermo Fisher Scientific Inc., Rochester, NY), and the culture medium without TPFC was exchanged for TPFC-containing culture medium just before confluence. The cells were exposed to 20 μg ¹⁰B/mL of TPFC for 24 h. After exposure, the glass slides were washed with 4°C PBS and the two-well chamber was removed. The nuclei specific Hoechest dye (Hoechst 33342; Lonza) was added (10 μg/mL) and the glass slides mounted onto cover glasses using DPX Mountant for histology (Fluka Biochemika 44581, Germany). The two-well chamber slides were observed using an inverted fluorescence microscope system (BZ-8000; KEYENCE, Japan).
The in vivo fluorescence microscopy study was conducted twenty four hours after termination of CED administration of TPFC to F98 rat glioma-bearing brain tumor model. The rats were euthanized and strip preparations of the brains were made. Similar to the in vitro fluorescence microscopy study, the strip preparations of brain were observed using an inverted fluorescence microscope system (BZ-8000; KEYENCE).

HIRAMATSU R., KAWABATA S., TANAKA H., SAKURAI Y., SUZUKI M., ONO K., MIYATAKE S.I., KUROIWA T., HAO E, & VICENTE M.G. (2014). Tetrakis(p-Carboranylthio-Tetrafluorophenyl)Chlorin (TPFC): Application for Photodynamic Therapy and Boron Neutron Capture Therapy. Journal of pharmaceutical sciences, 104(3), 962-970.

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Phenotypic Characterization of Peritoneal Cells

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The peritoneal cells were cultured in 8 well slide chambers under the same culture condition for weeks. After fixation with 4% Formaldehyde for 30 min, the cells were stained with FITC-conjugated anti-CD45 and PE-conjugated antiCD90 mAbs for 30 min and observed with a fluorescence stereomicroscope (BZ8000, Keyence, Osaka, Japan). For intracytoplasmic staining of Cytokeratin, Vimentin and FAP-α, the cells were permeablized by 0.02% triton-X after fixation, incubated with each mAb, and incubated with PE-conjugated secondary Abs.

Kitayama J., Emoto S., Yamaguchi H., Ishigami H, & Watanabe T. (2014). CD90(+) Mesothelial-Like Cells in Peritoneal Fluid Promote Peritoneal Metastasis by Forming a Tumor Permissive Microenvironment. PLoS ONE, 9(1), e86516.

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Angiogenic Potential of HUVECs on Matrigel

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Matrigel matrix were added into 24-well plates, solidified at 37°C for 30 min. Subsequently, cell suspensions of HUVECs (2×10⁵cell/mL) were added into each well. After 6 h of incubation in 37°C, 5% CO₂, HUVECs were stained with Calcein AM (0.25μg/mL) for 30 min, then observed under a fluorescence microscope (BZ-8000; Keyence, Osaka, Japan) at the excitation/emission wavelengths with 495/515 nm.

Jiang Y., Xu C.H., Zhao Y., Ji Y.H., Wang X.T, & Liu Y. (2023). LINC00926 is involved in hypoxia-induced vascular endothelial cell dysfunction via miR-3194-5p regulating JAK1/STAT3 signaling pathway. European Journal of Histochemistry : EJH, 67(1), 3526.

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Osteoclastogenesis Assay with RANKL and Tunicamycin

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CD14⁺ PBMCs (5 × 10⁴) were placed in a 96-well plate. The cells were incubated with RANKL (30 ng/ml) and M-CSF (25 ng/ml) for 2 weeks with or without tunicamycin treatment for the first 3 days. The medium was replaced every 3 days. Cultured cells were fixed with 10% formalin for 5 min and then with ethanol–acetone (50:50 vol/vol) for 1 min at room temperature. Tartrate-resistant acid phosphatase (TRAP) staining was performed using a TRAP/ALP Stain Kit (FUJIFILM Wako). Photographs were taken using BZ8000 (Keyence Co., Osaka, Japan). The number of TRAP-positive cells was counted for each number of nuclei (7 or 10 ≤ nuclei) (Piper et al., 1992 (link)). Areas of each TRAP-positive cell were measured using the ImageJ software (version 1.43; National Institute of Health, Bethesda, MD, United States).

Hayashi C., Fukuda T., Kawakami K., Toyoda M., Nakao Y., Watanabe Y., Shinjo T., Sano T., Iwashita M., Yotsumoto K., Shida M., Taketomi T., Sanui T., Uchiumi T., Kanematsu T, & Nishimura F. (2022). miR-1260b inhibits periodontal bone loss by targeting ATF6β mediated regulation of ER stress. Frontiers in Cell and Developmental Biology, 10, 1061216.

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Immunostaining of Phospho-AMPK in Oocytes

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Oocytes were denuded from the granulosa cells 8 or 20 h after CCCP treatment and immunostained as described previously (Takeo et al. 2013) (link). Rabbit polyclonal anti-phospho-AMPKa (Thr172) (1:1000; Cell Signaling Technology, Inc., Beverly, MA, USA) was used as a primary antibody and goat anti-rabbit IgG FITC-conjugate was used as a secondary antibody (1:1000; Millipore, Tokyo, Japan). The oocytes were mounted onto glass slides with antifade reagent containing DAPI. The fluorescence intensity of p-AMPK in oocytes was observed using a digital fluorescence microscope (BZ-8000; Keyence) and quantified using ImageJ Software (NIH, Bethesda, MD, USA).

Itami N., Shiratsuki S., Shirasuna K., Kuwayama T, & Iwata H. (2015). Mitochondrial biogenesis and degradation are induced by CCCP treatment of porcine oocytes. Reproduction (Cambridge, England), 150(2).

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Renal Superoxide Quantification Using DHE

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Dihydroethidium (DHE) was used to evaluate renal superoxide concentrations in situ, as described in detail elsewhere35 (link). After the reaction, the slide was observed using a Microscope (Keyence, BZ-8000, Osaka, Japan). DHE fluorescence of renal sections was quantified using the Image J analysis software. The mean fluorescence was quantified and expressed relative to values obtained in control rats.

Nishida K., Watanabe H., Ogaki S., Kodama A., Tanaka R., Imafuku T., Ishima Y., Giam Chuang V.T., Toyoda M., Kondoh M., Wu Q., Fukagawa M., Otagiri M, & Maruyama T. (2015). Renoprotective effect of long acting thioredoxin by modulating oxidative stress and macrophage migration inhibitory factor against rhabdomyolysis-associated acute kidney injury. Scientific Reports, 5, 14471.

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