Human colon epithelial cells (FHC), colon adenocarcinoma cell line (DLD-1), rectal adenocarcinoma cell line (HT29), colon cancer cell line (HCT116), and human microvascular endothelial cell line (HMEC-1) were purchased from ATCC, while the cecum adenocarcinoma cell line (NCI-H508) was purchased from the Center for basic Medical Cell, Institute of basic Medicine, Chinese Academy of Medical Sciences. The exosome-free fetal bovine serum (FBS) was produced by centrifugation (100,000 g) at 4°C overnight in order to ensure the removal of any bovine-derived exosomes [35 (link)]. The FHC cells were cultured in DMEM/F12 (Hyclone) medium containing 10% FBS. The DLD-1 cells were cultured in RPMI1640 medium containing 10% FBS, HT29 while the HCT116 cells were cultured in McCoy's 5A medium containing 10% FBS. The NCI-H508 cells were cultured in DMEM medium containing 10% FBS, and HMEC-1 cells were cultured in DMEM medium (31600-034, Hyclone, USA) comprised of 10% FBS (10099141, Gibco, USA) as well as streptomycin mixture. All cells were cultured at 37°C in a humidified atmosphere with 5% CO2. When cell confluency reached 90%, the cell passage procedure was performed accordingly.
Nci h508
Manufactured by American Type Culture Collection
Sourced in United States
The NCI-H508 is a cell line derived from a human colon adenocarcinoma. It is a commonly used in vitro model for studying colon cancer biology and evaluating potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by American Type Culture Collection (8 mentions)
Sw480, by American Type Culture Collection (7 mentions)
Dld 1, by American Type Culture Collection (6 mentions)
Sw620, by American Type Culture Collection (5 mentions)
Ls174t, by American Type Culture Collection (4 mentions)
Hct15, by American Type Culture Collection (4 mentions)
Caco 2, by American Type Culture Collection (4 mentions)
Ht 29, by American Type Culture Collection (3 mentions)
Nci h716, by American Type Culture Collection (3 mentions)
Sk co 1, by American Type Culture Collection (3 mentions)
Sw403, by American Type Culture Collection (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Snu 407, by Addexbio (2 mentions)
Ls1034, by American Type Culture Collection (2 mentions)
Snuc4 cell line, by Korean Cell Line Bank (2 mentions)
Dkk1 rdkk1, by R&D Systems (2 mentions)
Dkk3 rdkk3, by R&D Systems (2 mentions)
Xav939, by Selleck Chemicals (2 mentions)
Icg 001, by Selleck Chemicals (2 mentions)
17 protocols using nci h508
1
Cell Culture Protocols for Colon Cancer Research
2
Cultivation and Maintenance of Human and Murine Cell Lines
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Human embryonic kidney (HEK) 293 cells, human normal lung fibroblast cell line (HFL-1), human colon adenocarcinoma cell line (HCT-8), human colorectal carcinoma cell line (NCI-H508), human hepatocarcinoma cell line (Huh7), and murine melanoma cell line (YUMM5.2) were purchased from ATCC (Manassas, VA, USA). The human lung carcinoma cell line (A549), human cervical carcinoma cell line (Siha), and murine colon carcinoma cell line (CT26.WT) were bought from the Cell Bank of Shanghai Institutes for Biological Science (Shanghai, China). Murine colon adenocarcinoma cell line (MC38) was purchased from Shanghai Langzhi Biotech (Shanghai, China). These cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) and antibiotics and maintained in cell incubator with 5% CO2 at 37°C.kD
3
Colorectal Cancer Cell Lines Cultivation
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Colorectal cancer cell lines SW620 and HT29 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Colorectal cancer cell lines SW480, NCI‐H716, T84, COLO320HSR, SK‐CO‐1, and NCI‐H508 were purchased from ATCC (Manassas, VA, USA). COLO320HSR, NCI‐H508, and NCI‐H716 cells were maintained in RPMI‐1640 medium, SW480 and SW620 cells in Leibovitz's L‐15 medium, SK‐CO‐1 cells in minimal essential medium, T84 cells in a 1:1 mixture of Ham's F12 medium and DMEM, and HT‐29 cells in DMEM containing high glucose. All cells were supplemented with 10% FBS.
4
Colon Cancer Cell and Organoid Models
5
Colorectal Cancer Cell Line Cultivation
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Human colorectal cancer cell lines, including HCT116, HT29, RKO, DLD-1, SW403, SW1463, DiFi, and NCI-H508, were purchased from the ATCC and cultured in McCoy's 5A modified media. SNU-407 was purchased from AddexBio and cultured in RPMI1640 (Invitrogen). NCM356D non-transformed colonic epithelial cell line was purchased from INCELL and cultured in M3 media (INCELL). DR5-knockout (KO), PUMA-KO, and p53-KO HCT116 cells were described previously (20 (link), 27 (link)). Cells were authenticated by genotyping and analysis of protein expression by Western blotting, and routinely checked for Mycoplasma contamination by PCR. All cell lines were maintained at 37°C and 5% CO2 atmosphere. Cell culture media were supplemented with 10% defined FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. For drug treatment, cells were plated in 12-well plates at 20%–30% density 24 hours before treatment. DMSO stocks of NEO2734, CPI-637, I-BET151, OTX015, 5-FU, oxaliplatin, and SN-38 were prepared and diluted in cell culture media before adding to cells. All drugs were purchased from commercial sources, except for NEO2734 and CPI-637 which were provided by NEOMED Therapeutics 1, Inc.
6
Characterization of CRC Cell Lines
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The six CRC cell lines used in this study (HCT-15, HT115, LS1034, MDST8, NCI-H508, SNU-61) were previously selected to ideally represent the clinical phenotypes covered by TCGA as assessed by their transcriptional state inferred by VIPER, while also fulfilling practical culture condition considerations45 (link). The cell lines were obtained from ATCC (American Type Culture Collection) (HCT-15: ATCC#CCL-225, LS1034: ATCC#CRL-2158, NCI-H508: ATCC#CCL-253), the Korean Cell Line Bank (KCLB) (SNU-61: KCLB#00061), and the European Collection of Authenticated Cell Cultures (ECACC) (MDST8: ECACC#99011801, HT115: ECACC#85061104) and cultured using prescribed conditions to the amounts as described below. No authentication was conducted after purchase from the vendors. All cell lines were routinely tested for Mycoplasma contamination and were kept in a 37 °C humidity-controlled incubator with 5.0% CO2.
7
CRC Cell Line Culture and Co-culture
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The HCT116, SW620, NCI‐H508, and RKO human CRC and 293T HEK cell lines were obtained from the ATCC; the HCoEpiC human normal colonic epithelial cell line was obtained from Sciencell Research Laboratories. HCT116, HCoEpiC, and HEK 293T cell lines were cultivated in DMEM (Procell Life Science & Technology) supplemented with 10% FBS (WISENT) and 1% penicillin/streptomycin (New Cell & Molecular Biotech). SW620 and NCI‐H508 were cultivated in RPMI‐1640 (Procell Life Science & Technology), whereas RKO was cultivated in minimum essential medium (Procell Life Science & Technology). All cells were incubated at 37℃ in air enriched with 5% CO2. In addition, 500 nM SMO agonist SAG (MCE, HY‐12848B) was added to complete medium for coculturing with HCT116 for 24 hours in the incubator.
8
Colorectal Cancer Cell Line Cultures
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The human colorectal cancer cell lines DLD-1, HT-29, LoVo, NCI-H508, and Caco-2 were generous gifts from Dr. T. Azuma (Tokyo Dental College, Tokyo, Japan). The human colorectal cancer cell line SW480 was a generous gift from Dr. K. Nagano (The Institute of Medical Science, The University of Tokyo, Tokyo, Japan). DLD-1 and LoVo were originally purchased from JCRB Cell Bank (Osaka, Japan). HT-29, NCI-H508, Caco-2, and SW480 were from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cell lines, except SW480, were cultured in Dulbecco's modified Eagle’s medium ( DMEM; Wako, Osaka, Japan) supplemented with 10% fetal bovine serum (FBS), 4 mM L-glutamine, and penicillin/streptomycin. SW480 cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640; Wako, Osaka, Japan) supplemented with 10% FBS. FreeStyle 293F cells were cultured in FreeStyle 293 Expression medium (Life Technologies).
9
Cell Line Cultivation Protocols
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SW480, DLD-1, NCI-H508, and HEK293T cells were purchased from the American Type Culture Collection. Short tandem repeat analysis was performed for each of these cell lines. SW480, DLD-1, and HEK 293T cell lines were propagated in Dulbecco’s modified Eagle medium (Gibco, Shanghai, China); the NCI-H508 cell line was propagated in RPMI (Gibco, Shanghai, China). All cell lines were maintained in media supplemented with 10% fetal bovine serum (Biological Industries), 100 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, Logan, UT) in a humidified incubator at 37°C under 5% CO2.
10
Culturing Human Colon Cancer Cell Lines
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Human colon cancer cell lines including NCI-H508, Caco-2, CW-2 and HCT 116, and normal control cell line CCD-18Co were obtained from American Type Culture Collection (ATCC, USA). The cells were maintained in RPMI-1640 medium including 10% fetal bovine serum (FBS) and antibiotics (100 μg/mL streptomycin and 100 U/mL penicillin) under normal conditions.
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