Animals were sacrificed, and serial sections (18-24 μm in thickness) were prepared. Sections were blocked by PBS containing 0.3% Triton X-100 and 5% bovine serum albumin (BSA) for 1.5 h. Primary antibodies were incubated overnight as the followings: rabbit anti-NG2 antibody (1 : 200, Millipore), rabbit anti-CNPase (1 : 200, Millipore), rabbit anti-MBP antibody (1 : 200, GeneTex), rabbit anti-Ki67 antibody (1 : 200, GeneTex), rat anti-PDGFRα(1 : 500, Abcam), rabbit anticleaved caspase-3 (1 : 200, GeneTex), and goat anti-Sox10 antibody (1 : 100, Santa Cruz Biotech). After washing, sections were incubated with secondary antibodies conjugated with Alexa Fluor 488 (donkey anti-rabbit, 1 : 400, Molecular probes) or Alexa Fluor 594 (donkey anti-goat or anti-rat IgG, 1 : 800, Molecular probes) for 2-4 h at room temperature. The nuclei were stained by DAPI (1 : 1500, Sigma). Images were taken by using confocal microscope (FV3000, Olympus) with same setting to reduce variation.
Rabbit anti ki67 antibody
Manufactured by GeneTex
The Rabbit anti-Ki67 antibody is a tool for the detection and quantification of the Ki67 protein, which is a well-established marker of cellular proliferation. This antibody can be used in various immunodetection techniques, such as immunohistochemistry and immunofluorescence, to visualize and analyze the expression of Ki67 in biological samples.
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Lab products found in correlation
Rabbit anti ng2 antibody, by Merck Group (1 mentions)
Rabbit anti mbp antibody, by GeneTex (1 mentions)
Rat anti pdgfrα, by Abcam (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Fv3000, by Olympus (1 mentions)
Hrp conjugated anti rabbit igg, by GeneTex (1 mentions)
2 protocols using rabbit anti ki67 antibody
1
Immunohistochemistry of Oligodendrocyte Markers
2
Immunohistochemical Analysis of Ki67 in Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The harvested tumors and organs were fixed with 10% formalin and embedded in paraffin. Sample sections (6 μm) were stained with H&E for morphological observation. For immunohistochemical staining, tumor sections were deparaffinized and rehydrated before staining. The sections were treated with 0.3% H2O2 and incubated with blocking buffer (PBS containing 1% BSA and 0.25 Triton X-100) for 1 hr. Sections were incubated overnight with a rabbit anti-Ki67 antibody (1:400) (GeneTex International Corporation, Hsinchu City, Taiwan) at 4 ˚C. After PBS washing, sections were incubated with HRP-conjugated anti-rabbit IgG (1:1000) at room temperature for 1 h. Sections were washed with PBS and stained with diaminobenzidine (DAB) for 5–10 min at room temperature and counterstained with hematoxylin. Samples were dehydrated, and mounted with cover slides. The slides were observed under a microscopy at 10 × magnification.
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