3 4 methylenedioxymethamphetamine

Amphetamine, 4-fluoroamphetamine (4-FA), methcathinone, 4-fluoromethcathinone (4-FMC), 4-chloromethcathinone (4-CMC), and 3,4-methylenedioxymethamphetamine (MDMA) were purchased from Lipomed (Arlesheim, Switzerland). 4-Chloroamphetamine (PCA) was purchased from Cayman Chemical (Ann Arbor, MI, USA). All drugs were racemic hydrochloride salts with a HPLC purity of >98%. Test drugs were dissolved in DMSO and stored at -20 °C, then serially diluted in DMSO to avoid precipitation, followed by dilution in medium or assay buffer. The final DMSO concentration during the experiment was 0.1%.

Methanol (MeOH) and acetonitrile (AcN), LC–MS grade, were purchased from HiPerSolv CHROMANORM^®. LC–MS grade isopropanol (IPA) was obtained from Fisher Scientific International, Inc., Hampton, NH, USA. A Milli-Q purification system (18.2 MΩ cm⁻¹) was used to provide ultrapure water. Ammonium formate (AF) ≥99% and formic acid (FA) 98–100% mobile phase additives were purchased from Riedel-de Haën^® (Sigma-Aldrich, Steinheim, Germany) and ChemLab, Zedelgem Belgium, respectively. Reference standards of diazepam, bromazepam, temazepam, oxazepam, alprazolam, 7-aminoflunitrazepam (7-AF), methadone, 3,4-methylenedioxymethamphetamine (MDMA), cathine, mescaline, cocaine, benzoylecgonine, methylecgonine, cocaethylene, 25B-NB2OMe, 25C-NB2OMe, 25I-NB2OMe, mephedrone, AH-7921, 1-Benzylpiperazine, methylone, 3,4-Methylenedioxypyrovalerone (MDPV), JWH-018, tetrahydrocannabinol (THC), cannabinol (CBN), and cannabidiol (CBD) were of more than 98% purity and were purchased from Lipomed AG (Arlesheim, Switzerland).
Dried blood spots (qDBSs) devices were obtained from Capitainer AB^® (Solna, Sweden).