Telvision 11q d11s1037

FISH analysis was performed on cultured cells in 104/108 patients. In 4/108 patients, a formalin-fixed paraffin- embedded (FFPE) tumors were used. In six patients both type of samples were used. FFPE specimens were prepared with a Pretreatment Reagent Kit (Vysis Abbott Molecular, Downers Grove, IL, USA) according to the manufacturer’s protocol. For the routine diagnostics of patients with suspBL diagnoses, the following probes were used: BCL2 breakapart (BAP), BCL6 BAP, and MYC BAP (Vysis Abbott Molecular). For the precise evaluation of the MYC aberrations, the following probes were used: IGH BAP, IGH/MYC:CEP8 (Vysis Abbott Molecular), IGK/MYC, and IGL/MYC (CytoTest, Rockville, MD, USA). For the assessment of 11q gain/loss, the following panel of probes (11q gain/loss panel) was used: ATM SO, CCND1 SO, MLL BAP, TelVision 11q (D11S1037), and CEP11 (Vysis Abbott Molecular). The FISH results were analyzed using a fluorescence microscope, Axioskop2 (Carl Zeiss, Jena, Germany), documented by the ISIS Imaging System (Metasysytems, Altlussheim, Germany).