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Fluorescein isothiocyanate fitc conjugated cd45

Manufactured by Miltenyi Biotec

Fluorescein isothiocyanate (FITC)-conjugated CD45.2 is a lab equipment product. It is a fluorescent-labeled antibody that binds specifically to the CD45.2 antigen expressed on the surface of certain cell types.

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2 protocols using fluorescein isothiocyanate fitc conjugated cd45

1

Isolation and Characterization of Murine Hematopoietic Cells

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The following antibodies were purchased from BD Biosciences (San Diego, CA): anti-Fcγ-III/II receptor; APC-conjugated anti-mouse c-kit (clone 2B8); R-Phycoerythrin (PE)-conjugated anti-mouse CD3 (clone 143-2C11), GR-1 (clone RB6-8C5), B220 (clone RA3-6B2), Mac1 (clone M1/70) and Ter119 (clone Ter119); fluorescein isothiocyanate (FITC)-conjugated CD45.2 and PE-conjugated CD45.1; rat IgG2a; rat IgG2b and hamster IgG. Biotinylated lineage markers consisting of CD5, CD11b, Gr-1, 7-4 and Ter119 were purchased from Miltenyi Biotech (Auburn, CA). Recombinant human (rh) Flt3 ligand (FL), rh thrombopoietin (Tpo) and recombinant mouse stem cell factor (rmSCF) were purchased from R&D Systems (Minneapolis, MN). Recombinant murine GM-CSF (rmGM-CSF) was purchased from BioVision (Palo Alto, CA). Recombinant human erythropoietin was purchased from Amgen (Thousand Oaks, CA).
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2

Isolation and Characterization of Murine Hematopoietic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were purchased from BD Biosciences (San Diego, CA): anti-Fcγ-III/II receptor; APC-conjugated anti-mouse c-kit (clone 2B8); R-Phycoerythrin (PE)-conjugated anti-mouse CD3 (clone 143-2C11), GR-1 (clone RB6-8C5), B220 (clone RA3-6B2), Mac1 (clone M1/70) and Ter119 (clone Ter119); fluorescein isothiocyanate (FITC)-conjugated CD45.2 and PE-conjugated CD45.1; rat IgG2a; rat IgG2b and hamster IgG. Biotinylated lineage markers consisting of CD5, CD11b, Gr-1, 7-4 and Ter119 were purchased from Miltenyi Biotech (Auburn, CA). Recombinant human (rh) Flt3 ligand (FL), rh thrombopoietin (Tpo) and recombinant mouse stem cell factor (rmSCF) were purchased from R&D Systems (Minneapolis, MN). Recombinant murine GM-CSF (rmGM-CSF) was purchased from BioVision (Palo Alto, CA). Recombinant human erythropoietin was purchased from Amgen (Thousand Oaks, CA).
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