Anti myeloperoxidase mpo

Anti-phospho Smad2 (pSmad; Cat#AB3849), anti-Ki-67 (Cat# AB9260), HRP-conjugated anti-rabbit IgG (Cat# 12–348), and anti-vascular endothelial growth factor (VEGF; Cat# 05–443) were purchased from Millipore (Billerica, MA). Anti-caspase-3 antibody (Cat# 9661) was from Cell Signaling Technology (Danvers, MA). Anti-CD68 antibody (Cat# 6A324) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti- hypoxia-inducible factor 1-alpha (HIF-1α; Cat#NB-100-449) was purchased from Novus Biologicals (Littleton, CO). Anti-occludin (Cat#331500) and anti-ZO-1 (Cat#617300) antibodies were purchased from Invitrogen. Anti-granulocyte receptor-1 (Gr1; Cat# MBS520313) was from MyBioSource (San Diego, CA). Horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Cat# A4116), Cy3-conjugated anti-rabbit IgG (Cat# C2306) and anti-β-actin (Cat# A5441) antibodies were purchased from Sigma-Aldrich. Anti-myeloperoxidase (MPO) (Cat#DOM00001G), anti-claudin-3 (Cldn3; Cat# 341700), AlexaFlour 488-conjugated anti-mouse IgG (Cat# A11029), AlexaFluor 488-phalloidin (Cat# A12379), AlexaFluor 546-phalloidin (Cat# A22283), and anti-TNFα (Cat# AMC3012) antibodies were purchased from Thermo Fisher Scientific.

RNA iso Plus, SYBR Premix Ex TaqTM and PrimeScript RT Master Mix for real-time PCR (RT-PCR) were procured from TaKaRa Biotechnology Inc. (Toyobo, Japan). Anti-myeloperoxidase (MPO) was bought from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Anti-HMGB1, anti-claudin-5, anti-occludin, anti-TLR4, and anti-MyD88 were acquired from Abcam Biotechnology Inc. (Cambridge, UK). Anti-ZO-1, anti-mucin 2 (sc-15334), and anti-NF-κB p65 were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Their respective horseradish peroxidase-coupled secondary antibodies were obtained from KPL (Gaithersburg, MD, USA).