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5 protocols using anti rps19

1

Comprehensive Protein Expression Analysis Using Western Blotting

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Western blot analysis was performed using the following antibodies: anti-eIF3a (Novus NBP1-18891); anti-eIF3b (Bethyl A301-761A); anti-eIF3c (Bethyl A300-376A); anti-eIF3d (Bethyl A301-758A); anti-eIF3e (Bethyl A302-985A); anti-eIF3f (Bethyl A303-005A); anti-eIF3g antibody (Bethyl A301-757A); anti-eIF3h antibody (Bethyl A301-754A); anti-eIF3i (Biolegend 646701); anti-eIF3k (Novus NB100-93304); anti-eIF3l antibody (Genetex GTX120119); anti-eIF3m (Novus NBP1-56654); anti-rpS19 (Bethyl A304-002A); anti-eIF4G1 (Bethyl A301-775A); anti-c-Jun (Bethyl A302-959A); anti-BTG1 (Abcam ab151740). anti-GAPDH (Bethyl A300-640A); anti-HSP90 (BD 610418).
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2

Comprehensive Protein Expression Analysis Using Western Blotting

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Western blot analysis was performed using the following antibodies: anti-eIF3a (Novus NBP1-18891); anti-eIF3b (Bethyl A301-761A); anti-eIF3c (Bethyl A300-376A); anti-eIF3d (Bethyl A301-758A); anti-eIF3e (Bethyl A302-985A); anti-eIF3f (Bethyl A303-005A); anti-eIF3g antibody (Bethyl A301-757A); anti-eIF3h antibody (Bethyl A301-754A); anti-eIF3i (Biolegend 646701); anti-eIF3k (Novus NB100-93304); anti-eIF3l antibody (Genetex GTX120119); anti-eIF3m (Novus NBP1-56654); anti-rpS19 (Bethyl A304-002A); anti-eIF4G1 (Bethyl A301-775A); anti-c-Jun (Bethyl A302-959A); anti-BTG1 (Abcam ab151740). anti-GAPDH (Bethyl A300-640A); anti-HSP90 (BD 610418).
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3

Western Blot Antibody Characterization

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Western blot analyses were performed using the following antibodies: anti-eIF3d (Bethyl A301-758A), anti-eIF4A1 (Cell Signaling 2490), anti-eIF4G1 (Cell Signaling 2858), anti-rpS19 (Bethyl A304-002A), anti-eIF4E (Bethyl A301-154A), anti-rpLP0 (Bethyl A302-882A), anti-HA epitope tag (Pierce 26183), anti-c-Jun (Cell Signaling 9165), anti-Hsp90 (BD Biosciences 610418), and anti-4EBP1 (Cell Signaling 9644).
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4

Western Blot Antibody Characterization

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Western blot analyses were performed using the following antibodies: anti-eIF3d (Bethyl A301-758A), anti-eIF4A1 (Cell Signaling 2490), anti-eIF4G1 (Cell Signaling 2858), anti-rpS19 (Bethyl A304-002A), anti-eIF4E (Bethyl A301-154A), anti-rpLP0 (Bethyl A302-882A), anti-HA epitope tag (Pierce 26183), anti-c-Jun (Cell Signaling 9165), anti-Hsp90 (BD Biosciences 610418), and anti-4EBP1 (Cell Signaling 9644).
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5

Immunoblot Antibody Dilutions for Protein Analysis

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Immunoblot studies were performed using the following antibodies at 1:1000 dilution: anti-DAP5 (BD Biosciences 610742); anti-eIF3d (Bethyl A301-758A); anti-eIF4A1 (Cell Signaling 2490); anti-eIF4G1 (Cell Signaling 2858); anti-rpS19 (Bethyl A304-002A); anti-eIF4E (Bethyl A301-154A); anti-HA epitope tag (Abcam 18181); anti-c-Jun (Cell Signaling 9165); anti-CDK12 (Cell Signaling 11973); anti-MMP1 (Abcam 137332); anti-GAPDH (Cell Signaling 2118S); anti-4E-BP1 (Cell Signaling 9644); anti-Lumin A (Abcam 226189); anti-AUF1 (EMD Millipore 07260); anti FXR1 (Cell Signaling 12295); anti FXR2 (Cell Signaling 7098); and anti-PABP (Cell Signaling 4992). The following antibodies were used at 1:500 dilution: anti-ETS1 (Abcam 26096); anti-OSMR (Abcam 210771); anti-EGFR (Cell Signaling 4257); anti-SERPINE2 (Abcam 75348); anti-ITGV (Abcam 124968); and anti-p53-BP1 (Abcam 21083).
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