Arvo mx1420 microplate reader

Cells were plated in tissue culture plates and test compounds were added simultaneously. After the treatment period, cells were lysed with CellTiter Glo (Promega) and the luminescent signal was measured using an ARVO MX1420 Microplate Reader (Perkin-Elmer, Waltham, MA, USA).

Cell viability was determined using the CellTiter-Glo luminescence assay (Promega, Madison, WI, USA) according to the manufacturer's suggested protocol. Luminescent signals were detected using an ARVO MX1420 microplate reader (PerkinElmer, Wellesley, MA, USA). Cell numbers were determined using a TC10 automated cell counter (Bio-Rad Laboratories, Hercules, CA, USA). In general, cancer cells were reversely transfected with siRNA and cell proliferation or cell numbers were determined 96 h after siRNA transfection. Caspase-3/7 activity was determined using the Caspase-Glo 3/7 Assay (Promega). Cellular senescence was assessed using the SA-b-Gal Staining kit from Cell Biolabs (Cat. No. CBA-230, San Diego, CA, USA).