Blood samples were inoculated into BacT-ALERT FA Plus bottles and incubated on a BacT-ALERT VIRTUO blood culture system (BioMérieux, Durham, NC, USA). When a blood bottle was flagged as positive by the instrument, it was subjected to Gram staining, and a preliminary result was reported. The sample was then sub-cultured on sheep blood agar and MacConkey agar (Asan Pharmaceutical, Hwaseong, Korea). After overnight incubation at 35°C, all bacterial species were identified using Microflex with a Biotyper IVD MBT v.2.3 matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system (Bruker Daltonics, Bremen, Germany), according to the manufacturer’s instructions.
Bact alert virtuo blood culture system
Manufactured by bioMérieux
Sourced in United States, France
The BacT-ALERT VIRTUO blood culture system is a diagnostic instrument designed for the automated detection and monitoring of microbial growth in blood culture samples. It utilizes a continuously monitored colorimetric detection method to identify the presence of microorganisms in the samples.
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2 protocols using bact alert virtuo blood culture system
1
Automated Blood Culture Identification
2
Comprehensive Blood Culture and Pathogen Identification
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The BACT/ALERT VIRTUO blood culture system (BioMérieux, Marseille-L’Étoile, France), Bactec FX blood culture system (Becton-Dickinson, Sparks, MD, USA), and their supporting blood culture bottles were used for blood culture. The Vitek 2 Compact system (BioMérieux, Marseille-L’Étoile, France) and Autof ms1000 (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) (Autobio Diagnostics, Zhengzhou, China) were used for the identification of bacteria and fungi. The markers of PGN, LPS, and BDG were measured by Gram-positive bacterium Peptidoglycan Determination Assay (Chromogenic) (Approval number: 20192400460), Gram-negative Bacterial Endotoxin Chromogenic Assay (Approval number: 20192400268), Fungus (1,3)-β-D-glucan Chromogenic Assay (20192400281), and the GLP-F300 automatic enzyme labeling analyzer (Approval number: 20202221003) (Autobio Diagnostics, Zhengzhou, China).
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