Sprague-Dawley rats, Swiss albino mice, newly synthesized nanoassemblies (PFT-HSA-TFT-Cy7 and PFT-Hcy-HSA-Cy7), CML detection ELISA kit (bioassay technology laboratory), TRIzol Reagent (Ambion), primers (Eurofins), Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific), real-time PCR (Himedia). Hi-SYBr Master Mix, Agarose, Tris buffer, ethidium bromide, bromophenol blue, Protein Estimation Kit (G Biosciences), DNPH, methylglyoxal, bovine serum albumin, PBS, antibiotic antimycotic solution, and trypsin (all these chemicals were purchased from Himedia), glucometer (One Touch Select simple), 0.2 μm syringe filter (Sartorius), DCFDA dye, nitrosodiethylamine, alloxan monohydrate (all these chemicals were purchased from Sigma Aldrich), UV-spectrophotometer (Eppendorf), spectrofluorometer (Agilent technologies, Carry Eclipse), phenobarbitone (Abbot), RPMI (Cell Clone), fetal bovine serum (Gibco), and RNA later (Invitrogen).
Methylglyoxal
Manufactured by HiMedia
Sourced in India
Methylglyoxal is a chemical compound with the formula CH3COCHO. It is a colorless, volatile liquid with a pungent odor. Methylglyoxal is used as a laboratory reagent and intermediate in organic synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by HiMedia (2 mentions)
Thiobarbituric acid, by HiMedia (2 mentions)
Trichloroacetic acid, by HiMedia (2 mentions)
Sodium carbonate, by HiMedia (2 mentions)
Oxalic acid, by HiMedia (2 mentions)
Phosphotungstic acid, by HiMedia (2 mentions)
Sodium chloride (nacl), by HiMedia (2 mentions)
Sodium hydroxide (naoh), by HiMedia (2 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (2 mentions)
Nitrosodiethylamine, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Alloxan monohydrate, by Merck Group (1 mentions)
Uv spectrophotometer, by Eppendorf (1 mentions)
0.2 μm syringe filter, by Sartorius (1 mentions)
Rnalater, by Thermo Fisher Scientific (1 mentions)
Real time pcr, by HiMedia (1 mentions)
Phosphate buffered saline (pbs), by HiMedia (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution, by HiMedia (1 mentions)
4 protocols using methylglyoxal
1
Evaluation of Nanoassemblies for CML Detection
2
Chitosan-based Antioxidant Nanoparticles
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Chitosan powder (deacetylation degree >85%), aqueous acetic acid, dichloromethane (DCM), sodium-tripolyphosphate (S-TPP), chloroform, Tween-20, Tween-80, acetic acid (99.99% purity), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid; ABTS), 1,2-diaminobenzene (DAB), thiobarbituric acid (TBA), trichloroacetic acid (TCA), methylglyoxal, perchloric acid, sodium carbonate, and nutrient media (PDA and SMKY) were purchased from HiMedia laboratories, Mumbai, India. The EO of A. rosaeodora was procured from MRK naturals (99.90% purity), New Delhi, India.
3
Optimization of Protein Modification Assays
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Fibrinogen, methylglyoxal, oxalic acid, thiobarbituric acid (TBA), trichloroacetic acid (TCA), sodium chloride (NaCl), sodium hydroxide (NaOH), phosphotungstic acid, bovine serum albumin (BSA), ammonium persulphate (APS), bis-acrylamide, and tetramethylethylenediamine (TEMED) were purchased from Hi-Media. Sodium dodecyl sulfate (SDS) and 2,4,-trinitrobenzene-1-sulphonic acid (TNBS) were obtained from G Biosciences, whereas ethyl acetate and dinitrophenylhydrazine (DNPH) were purchased from Rankem. Guanidium hydrochloride was obtained from S. D. Fine-Chem Limited, and hydrochloric acid (HCl) was purchased from Fisher Scientific. A protein A agarose column was purchased from Sigma, and polystyrene plates were obtained from Nunc (Denmark). Analytical grade chemicals were used in this study. All the solutions were filtered under aseptic conditions using a sterilized Puradisc™ 0.2 mM syringe filter (Whatman, GE Healthcare UK Limited, UK).
4
Isolation and Purification of C-PC from Cyanobacteria
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C-PC was isolated and purified from Plectonema sp. of cyanobacteria as described in our previous reports (13) (link) and BSA was obtained from Hi-media, sodium carbonate, sodium dihydrogen phosphate, sodium bicarbonate, disodium hydrogen phosphate, methylglyoxal, sodium chloride, oxalic acid, nitroblue tetrazolium (NBT), thiobarbituric acid (TBA), trichloroacetic acid (TCA), sodium hydroxide, phosphotungstic acid, sodium dodecyl sulphate (SDS) and sodium chloride were purchased from Hi-Media. Sodium azide (Na 3 N) and ethanol were obtained from Merck and 2,4,6 trinitrobenzene sulphonic acid (TNBS) was purchased from G-Biosciences. Thioflavin-T (ThT), congo red, phenanthrenequinone, 1-anilinonapthalene-8-sulfonic acid, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide [K 4 Fe(CN) 6 ], ferric chloride (FeCl 3 ), sodium nitroprusside, sulfanilic acid, naphthalene diamine chloride and triphenylphosphine were purchased from Sigma Aldrich. Ethylacetate was purchased from Emplura, and dinitrophenylhydrazine (DNPH) was purchased from Rankem. Guanidium hydrochloride was purchased from SD Fine Chemical Limited. Hydrochloric acid (HCl) was purchased from Fisher Scientific. All other chemicals used in this study were of analytical grade.
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