Mirtazapine (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was dissolved in 0.5% methylcellulose. Healthy male ICR mice weighing 38–42 g (8 weeks old) were intraperitoneally (i.p.) injected with Mirtazapine (5 or 16 mg/kg) once per day for 8 days. One day after the final injection, the mice were perfused transcardially with 4% paraformaldehyde (PFA) under deep anesthesia with sodium pentobarbital (80 mg/kg, i.p.) for immunohistochemical analysis.
Mirtazapine
Manufactured by Fujifilm
Sourced in Japan
Mirtazapine is a laboratory equipment product manufactured by Fujifilm. It is designed for use in various research and analytical applications. The core function of Mirtazapine is to provide reliable and consistent performance in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Methyl cellulose 400, by Fujifilm (2 mentions)
Escitalopram oxalate, by Merck Group (1 mentions)
Venlafaxine hydrochloride, by Merck Group (1 mentions)
Duloxetine hydrochloride, by Fujifilm (1 mentions)
2 7 dichlorofluorescin diacetate dcfda, by Merck Group (1 mentions)
Sertraline hydrochloride, by Fujifilm (1 mentions)
96 well plate, by Corning (1 mentions)
4 protocols using mirtazapine
1
Mirtazapine administration in mice
2
Assessment of Antidepressant Drug Effects
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Ham’s F-12K medium (F-12K), sertraline hydrochloride, paroxetine hydrochloride, duloxetine hydrochloride, fluvoxamine maleate, and mirtazapine were obtained from Fujifilm Wako Pure Chemical Industries Ltd. (Osaka, Japan). Escitalopram oxalate, venlafaxine hydrochloride, and 2′,7′-dichlorofluorescin diacetate (DCFDA) were obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Apotracker Green (Apo-15) and Calcein Red-acetoxymethyl ester (Calcein Red-AM) were purchased from BioLegend, Inc. (San Diego, CA, USA). All other chemicals used in the study were of the highest purity available. The 96-well plates were obtained from Corning (Corning, NY, USA).
3
Synthesis and Administration of Suvorexant and Mirtazapine
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Suvorexant ([(7R)-4-(5-chloro-1,3-benzoxazol-2-yl)-7-methyl-1,4-diazepan-1-yl][5-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl] methanone) was synthesized as described in previous studies [30 , 31 (link)]. 30mg/kg suvorexant was dissolved in 0.5% methylcellulose 400 (Wako Pure Chemicals, Japan) immediately before oral administration. Mirtazapine was purchased from. 1mg/kg Mirtazapine (Wako Pure Chemicals, Osaka, Japan) was dissolved in 5% dimethyl sulfoxide and 5% Tween80 before intraperitoneal injection. Methylcellulose and dimethyl sulfoxide were administrated as vehicle controls for suvorexant and Mirtazapine, respectively.
4
Suvorexant Synthesis and Administration
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Suvorexant ([(7R)-4-(5-chloro-1,3-benzoxazol-2-yl)-7-methyl-1,4-diazepan-1-yl][5-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl] methanone) was synthesized according to previous studies [45, (link)46] . It was dissolved in 0.5% methyl cellulose 400 (Wako Pure Chemicals, Japan) immediately before oral administration. Mirtazapine was purchased from Wako Pure Chemicals (Osaka, Japan) and dissolved in 5% dimethyl sulfoxide and 5% Tween80 just before intraperitoneal injection.
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