Triglyceride tg colorimetric assay kit

Ten larvae or flies (one sample) were homogenized in 150 μL cold isopropanol. The homogenized samples were then centrifuged at 12,000 g for 10 min at 4 ℃. Next, triglyceride (TAG) levels were assayed using the Triglyceride (TG) Colorimetric Assay Kit (Elabscience) according to the manual. Protein contents were determined by the BCA Protein Quantification Kit for normalization.

CS271011 was designed and synthesized at Chipscreen Biosciences (Shenzhen, Guangdong, China). The purity of CS271011 was over 99%. Resmetirom (MGL-3196) was purchased from MedChemExpress (Hycultec GmbH, Beutelsbach, Germany). Compounds were dissolved and suspended in sterile DMSO and 0.2% CMC-Na for in vitro and in vivo administration, respectively. The chow diet was supplied by Guangdong Medical Laboratory Animal Center (Foshan, Guangdong, China), and the high-fat diet (D12492i) was provided by SYSE Biotech Co., Ltd. (Changzhou, Jiangsu, China). Cholesterol (TC), alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and total bilirubin (TBIL) diagnostic kits were purchased from the Jiancheng Bioengineering Institute of Nanjing (Nanjing, Jiangsu, China). The Triglyceride (TG) Colorimetric Assay Kit was purchased from Elabscience (Wuhan, Hubei, China). All primers were synthesized by Genewiz (Suzhou, Jiangsu, China).

HK-2 cells were inoculated in 6-well plates at 5 × 10⁵ cells per well. We treated cells with TPA for 48 h with or without rklotho. At the end of treatment, cells were broken using Scientz-IID Ultrasonic Cell Crusher (Scientz, China), and the cell supernatants were collected for triglyceride measurement. Triglyceride concentrations in cell supernatants were determined using a triglyceride (TG) colorimetric assay kit (Elabscience, China) according to the manufacturer's instructions.