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Odysseyxl system

Manufactured by LI COR
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The OdysseyXL system is a high-performance imaging system designed for a variety of life science applications. It features a large imaging area, advanced optics, and sensitive detection capabilities to support a range of sample types and imaging needs.

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Lab products found in correlation

Caveolin 1, by Abcam (2 mentions) Collagen 3, by Abcam (2 mentions) Criterion gel system, by Bio-Rad (2 mentions) Trans blot turbo system, by LI COR (2 mentions) 800cw or 680cw ir dye, by LI COR (2 mentions) Timp 1, by Abcam (2 mentions) Timp 2, by Abcam (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Collagen 1, by Abcam (2 mentions) Brain derived neurotrophic factor (bdnf), by Abcam (1 mentions) Epac1, by Abcam (1 mentions) Epac2, by Abcam (1 mentions) Trans blot turbo rapid transfer system, by Bio-Rad (1 mentions) β actin, by Merck Group (1 mentions) Irdye 680cw, by LI COR (1 mentions) Irdye 800cw, by LI COR (1 mentions) Odyssey system, by LI COR (1 mentions)

4 protocols using odysseyxl system

1

Quantitative Analysis of Cell Signaling

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Whole cell lysates or concentrated conditioned media were separated on SDS-PAGE gels (7%, 10%, or 4-15% gradient gels, Criterion Gel System; Bio-Rad, Hercules, CA) via standard techniques. Proteins were transferred to nitrocellulose membranes using a Bio-Rad Trans-Blot Turbo system and blocked in Odyssey Blocking buffer (Li-Cor Biosciences, Lincoln, NE) prior to overnight incubation in a 1μg/mL solution of primary antibody of interest. Primary antibodies included: caveolin-1, collagen I, collagen III (Abcam, Cambridge, MA), Cavin-1, Cavin-3, fibronectin, matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 9 (MMP9), tissue inhibitor of metalloproteinase 1 (TIMP1), and tissue inhibitor of metalloproteinase 2 (TIMP2) (Santa Cruz Biotechnology, Santa Cruz, CA). Secondary antibodies were conjugated to 800CW or 680CW IR dye (Li-Cor Biosciences) and imaging was performed using a Li-Cor OdysseyXL system prior to quantification via densitometry. GAPDH expression was used to normalize cell lysate protein concentrations. Concentrated conditioned media was normalized to cell lysate protein concentrations as determined by Lowry protein assays.
Vogel E.R., Britt RD J.r., Faksh A., Kuipers I., Pandya H., Prakash Y., Martin R.J, & Pabelick C.M. (2016). Moderate Hyperoxia Induces Extracellular Matrix Remodeling by Human Fetal Airway Smooth Muscle Cells. Pediatric research, 81(2), 376-383.
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2

Protein Quantification and Characterization

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Cells were harvested using standard techniques (cell lysis buffer containing protease inhibitors). Protein was separated by SDS PAGE using a 4–15% Tris-HCl gel and transferred to nitrocellulose membranes using a Bio-Rad Trans-Blot Turbo rapid transfer system (Bio-Rad, Hercules, CA). Primary antibodies used include BDNF, Epac1, Epac2 (Abcam, Cambridge, MA), and β-actin (Sigma-Aldrich, St. Louis, MO). Membranes were imaged on a Li-Cor OdysseyXL system and densitometry was quantified with Image Studio software. Protein blots were normalized to β-actin.
Thompson M.A., Britt RD J.r., Kuipers I., Stewart A., Thu J., Pandya H.C., McFarlane P., Pabelick C.M., Martin R.J, & Prakash Y.S. (2015). cAMP-Mediated Secretion of Brain-Derived Neurotrophic Factor in Developing Airway Smooth Muscle. Biochimica et biophysica acta, 1853(10 0 0), 2506-2514.
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3

Immune-ECM Quantification Assay

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The cells were plated at 5X104 cells per well in 96 well plates until 70–90% confluence for treatment. After 48 h treatment, the cells were ready for the immune-ECM assay. The culture media was gently removed from the samples, followed by two washes with PBS. Samples were fixed in 4% paraformaldehyde for 15 min. After two additional PBS washes, cells were treated with Odyssey Blocking Buffer for 45 min. Cells were incubated with primary polyclonal rabbit antibody for collagen I (Novus NB600–408) or fibronectin (Santa Cruz, sc-81767) diluted 1:200 in blocking buffer at 4 °C overnight. Cells were washed twice in PBS and incubated with 100uL of the corresponding secondary antibodies solution, which were prepared from IRDye® 800CW (Li-Cor, 926–32211) and IRDye® 680CW (Li-Cor, 926–68070) secondary antibodies in blocking buffer solution at a 1:750 ratio. The plate was imaged on the Li-Cor Odyssey system. Plates were imaged via a Li-Cor OdysseyXL system with quantification performed via densitometry and normalized with cell density. Data are expressed as IR intensity fold changes relative to control.
Liu W., Meridew J.A., Aravamudhan A., Ligresti G., Tschumperlin D.J, & Tan Q. (2019). Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression. Respiratory Research, 20, 281.
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4

Quantitative Analysis of Cell Signaling

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Whole cell lysates or concentrated conditioned media were separated on SDS-PAGE gels (7%, 10%, or 4-15% gradient gels, Criterion Gel System; Bio-Rad, Hercules, CA) via standard techniques. Proteins were transferred to nitrocellulose membranes using a Bio-Rad Trans-Blot Turbo system and blocked in Odyssey Blocking buffer (Li-Cor Biosciences, Lincoln, NE) prior to overnight incubation in a 1μg/mL solution of primary antibody of interest. Primary antibodies included: caveolin-1, collagen I, collagen III (Abcam, Cambridge, MA), Cavin-1, Cavin-3, fibronectin, matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 9 (MMP9), tissue inhibitor of metalloproteinase 1 (TIMP1), and tissue inhibitor of metalloproteinase 2 (TIMP2) (Santa Cruz Biotechnology, Santa Cruz, CA). Secondary antibodies were conjugated to 800CW or 680CW IR dye (Li-Cor Biosciences) and imaging was performed using a Li-Cor OdysseyXL system prior to quantification via densitometry. GAPDH expression was used to normalize cell lysate protein concentrations. Concentrated conditioned media was normalized to cell lysate protein concentrations as determined by Lowry protein assays.
Vogel E.R., Britt RD J.r., Faksh A., Kuipers I., Pandya H., Prakash Y., Martin R.J, & Pabelick C.M. (2016). Moderate Hyperoxia Induces Extracellular Matrix Remodeling by Human Fetal Airway Smooth Muscle Cells. Pediatric research, 81(2), 376-383.
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