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Filtermax f5 multi mode microplate reader

Manufactured by Molecular Devices
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Sourced in United States, Japan

The FilterMax F5 Multi-Mode Microplate Reader is a compact and versatile lab equipment designed for absorbance and fluorescence measurements. It features a high-performance optical system and supports 6- to 384-well microplates. The instrument can perform a range of detection modes, including endpoint, kinetic, and spectral scanning.

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Lab products found in correlation

Sytox green, by Thermo Fisher Scientific (5 mentions) Celltiter glo luminescent cell viability assay, by Promega (4 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Luciferase assay system, by Promega (3 mentions) Softmax pro 6, by Molecular Devices (3 mentions) Costar plate, by Corning (3 mentions) Hrp goat anti mouse igg fcγ fragment specific secondary antibody, by Jackson ImmunoResearch (2 mentions) Maxisorp 96 well elisa plate, by Thermo Fisher Scientific (2 mentions) Hrp goat anti mouse igg secondary antibody, by Merck Group (2 mentions) Dual luciferase reporter assay system, by Promega (2 mentions) C2 ceramide, by Cayman Chemical (2 mentions) 384 black well polystyrene microplates, by Corning (2 mentions) Retrotek htlv p19 antigen elisa, by ZeptoMetrix (2 mentions) Amersham protran western blotting nitrocellulose membranes, by Cytiva (2 mentions) Mini protean tgx precast protein gel, by Bio-Rad (2 mentions) Amersham imager 600, by GE Healthcare (2 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Merck Group (2 mentions) Complete mini protease inhibitor cocktail, by Roche (2 mentions) Sytox green probe, by Thermo Fisher Scientific (2 mentions)

116 protocols using filtermax f5 multi mode microplate reader

1

ABCG2 Targeted Exon Sequencing

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Genomic DNA was extracted from the whole peripheral blood cells of the participants.7 (link) We performed targeted exon sequencing of ABCG2 with a pool and capture method described in a previous study.8 (link) Briefly, the extracted DNA was quantified using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Waltham, Massachusetts, USA) on FilterMax F5 Multi-Mode Microplate Readers (Molecular Devices, Sunnyvale, California, USA). Twenty nanogram of DNA was simultaneously fragmented and ligated with adapters using the SureSelect QXT Library Prep Kit (Agilent Technologies, Santa Clara, California, USA). The 96 fragmented libraries with distinct indexed adapters were pooled in equimolar amounts. Target enrichment was then performed using the SeqCap EZ choice system (Roche Diagnostics, Tokyo, Japan). A DNA probe set complementary to the target region was designed using NimbleDesign (https://design.nimblegen.com). The libraries were sequenced on an Illumina HiSeq 2500 platform in rapid run mode with 2×150 bp paired-end modules (Illumina).
Higashino T., Takada T., Nakaoka H., Toyoda Y., Stiburkova B., Miyata H., Ikebuchi Y., Nakashima H., Shimizu S., Kawaguchi M., Sakiyama M., Nakayama A., Akashi A., Tanahashi Y., Kawamura Y., Nakamura T., Wakai K., Okada R., Yamamoto K., Hosomichi K., Hosoya T., Ichida K., Ooyama H., Suzuki H., Inoue I., Merriman T.R., Shinomiya N, & Matsuo H. (2017). Multiple common and rare variants of ABCG2 cause gout. RMD Open, 3(2), e000464.
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2

Quantifying Advanced Oxidation Protein Products

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The advanced oxidation protein products (AOPPs) were determined in heart tissue by spectrophotometry. The heart tissue was homogenized and diluted (1:5) in phosphate-buffered saline (PBS; pH 7.4). Next, 200 μL of the sample was added to a 96-well plate together with 10 μL of potassium iodide (1.16 M) and 20 μL of glacial acetic acid. The reading was taken immediately at 340 nm with an ELISA reader (Filter Max F5 Multi-Mode Microplate Readers, Molecular Devices, Silicon Valley, CA, United States). The blank was prepared by replacing the sample with PBS. The AOPP concentrations are expressed as μM of chloramine-T/mg of protein (Valente et al., 2013 (link)).
de Almeida S.A., Claudio E.R., Mengal V., Brasil G.A., Merlo E., Podratz P.L., Graceli J.B., Gouvea S.A, & de Abreu G.R. (2018). Estrogen Therapy Worsens Cardiac Function and Remodeling and Reverses the Effects of Exercise Training After Myocardial Infarction in Ovariectomized Female Rats. Frontiers in Physiology, 9, 1242.
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3

Optimized Azospirillum brasilense Growth Medium

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A modified minimal media for Azospirillum brasilense (MMAB, [72] ) was used for all experiments containing K2HPO4 (3 g L -1 ), NaH2PO4 (1 g L -1 ), KCl (0.15 g L -1 ),
NH4Cl (1 g L -1 ), MgSO4 • 7H2O (0.3 g L -1 ), CaCl2 • 2H2O (0.01 g L -1 ), FeSO4 • 7H2O (0.0025 g L -1 ), Na2MoO4• 2H2O (0.05 g L -1 ), and 5 g L -1 D-glucose as a carbon source.
10 ml of trace salt solution was added per liter of MMAB media from the 1L stock.
Trace salt stock solution consisted of filter-sterilised 84 mg L -1 of ZnSO4 The next day, precultures were diluted to an optical density of 0.1 at 600 nm as determined by FilterMax F5 multi-mode microplate readers (Molecular Devices).
Giri S., Oña L., Waschina S., Shitut S., Yousif G., Kaleta C., & Kost C. (2020). Metabolic dissimilarity determines the establishment of cross-feeding interactions in bacteria.
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4

Colorimetric Quantification of Ammonia and Nitrite

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Ammonia and nitrite concentrations were measured colourimetrically according to previously published protocols using Nessler’s reagent (Meseguer-Lloret et al., 2002 ) and Griess reagent (Miranda et al., 2001 (link)), respectively. Absorbance values were measured at 550 nm (nitrite) and 450 nm (ammonia) using a Filtermax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). All technical measurements were performed in duplicate. Substrate concentrations were determined by comparison to standards using SoftMax Pro 6.4 (Molecular Devices). Note that for reported ammonia concentrations, values refer to total ammonia (NH4+ + NH3), unless otherwise indicated.
Sauder L.A., Albertsen M., Engel K., Schwarz J., Nielsen P.H., Wagner M, & Neufeld J.D. (2017). Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system. The ISME Journal, 11(5), 1142-1157.
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5

ELISA Screening of Recombinant Proteins

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Microtiter plates (Nunc Maxisorp 96-well ELISA plates) were coated with 100 μl of denatured E.coli-expressed ZNS1 (500 ng/well) for hybridoma screening, or with 100 μl of the indicated concentration of denatured E.coli-expressed NS1 proteins, or native or denatured HEK293-expressed ZNS1 in coating buffer (0.1 M Na2HPO4 buffer pH 9.5) for 18 h at 4°C. Following incubation in blocking buffer (5% skimmed milk in TBS) for 1 h at 37°C, the plates were further incubated with hybridoma supernatants for screening, or with 0.5 μg/ml of the indicated purified mAb for 1 h at RT in blocking buffer. Following four washing steps in TBS Tween-20 0.05%, plates were further incubated for 1 h at RT with HRP goat anti-mouse IgG Fcγ fragment specific secondary antibody (Jackson Immunoresearch) at a 1:6000 dilution. Finally, plates were washed four times in TBS Tween-20 0.05% and after incubation with the substrate [0.3% H2O2, 0.1% 3,3’,5,5’-tetramethylbemzidine (TMB) in 0.1 M citric acid pH 5] for 10 to 30 minutes at RT, the reaction was stopped with 0.2 M H2SO4. The absorbance at 450 nm was measured with a FilterMax F5 Multi-Mode microplate reader (Molecular Devices).
Roldán J.S., Cassola A, & Castillo D.S. (2021). Development of a novel NS1 competitive enzyme-linked immunosorbent assay for the early detection of Zika virus infection. PLoS ONE, 16(8), e0256220.
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6

Resazurin-based Cell Viability Assay

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A working solution of 60 µM resazurin was prepared in a medium containing 1% FBS on the day of analysis according to a previously described method37 (link). After 6, 24, and 48 h of treatment of cells with increasing concentrations of the Les-236 compound in 96-well culture plates, the cells in the wells were replaced by a working solution of resazurin (100 µL) and the plates were incubated at 37 °C. Fluorescence was measured at an excitation wavelength of 530 nm and an emission wavelength of 590 nm on the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Corp., Sunnyvale, CA, USA) after 30 and 60 min of dye addition.
Szychowski K.A., Kaminskyy D.V., Leja M.L., Kryshchyshyn A.P., Lesyk R.B., Tobiasz J., Wnuk M., Pomianek T, & Gmiński J. (2019). Anticancer properties of 5Z-(4-fluorobenzylidene)-2-(4-hydroxyphenylamino)-thiazol-4-one. Scientific Reports, 9, 10609.
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7

Quantifying Liver Enzymes and Cytokines

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The presence of alanine aminotransferase (ALT) in the serum samples was determined using the Alanine Aminotransferase (ALT/GPT) Colorimetric Assay Kit (Elabscience, cat #: E-BC-K325). Samples were prepared according to the manufacturer's instructions, acquired on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) and analyzed using the SoftMax Pro software (Molecular Devices). The production of cytokines IL-10, IFNγ, and TNFα in blood plasma or conditioned media was measured using Cytometric Bead Array (CBA) kit (BD Biosciences). Samples were prepared according to the manufacturer's instructions, acquired on LSRFortessa (BD Biosciences) and analyzed using the FCAP Array software (BD Biosciences).
Ali A.K., Komal A.K., Almutairi S.M, & Lee S.H. (2019). Natural Killer Cell-Derived IL-10 Prevents Liver Damage During Sustained Murine Cytomegalovirus Infection. Frontiers in Immunology, 10, 2688.
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8

Neutrophil Chemotaxis Assay Protocol

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Purified hPMNs were re-suspended in mHBSS. Calcein AM dye re-suspended in DMSO (Sigma-Aldrich D2650) was added to the neutrophils at a final concentration of 3 μM, and cells were incubated in the dark for 20 minutes to fluorescently label the neutrophils. Labeled hPMNs were pelleted by spinning at 1500 RPM at 25°C for 5 minutes without accelerator and brake. The supernatant was removed and neutrophils were re-suspended in mHBSS. hPMNs were again pelleted without the accelerator and brake. After this, the supernatant was removed and cells were re-suspended at 106 hPMNs/ml. 200 μl of the appropriate chemotactic stimulus was added to the flat-bottom, black-walled, 96-well plates (Corning 3583) in triplicate. Fluorescently labeled neutrophils were then loaded into a 96-well transwell plate with 3.0 μM pore size (Corning 3385); 105 hPMNs were loaded per well. The transwell plate was placed in the black-bottom plate and incubated at 37°C with 5% CO2 for 45 minutes to allow migration. After migration, 50 μl of 0.5 M EDTA was placed in each of the lower wells and 10 μl of 0.5 M EDTA was placed in the upper wells. The plates were incubated at 4°C for 15 minutes after which the transwells were removed and the amount of fluorescence in the lower wells was quantified using a Molecular Devices Filter Max F5 Multi-Mode Microplate Reader.
Lorenzini J., Fites J.S., Nett J, & Klein B.S. (2017). Blastomyces dermatitidis serine protease dipeptidyl peptidase IVA (DppIVA) cleaves ELR+CXC chemokines altering their effects on neutrophils. Cellular microbiology, 19(9), 10.1111/cmi.12741.
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9

Glyco-iELISA for Detecting Antibodies Against AcrA

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Glyco-iELISA was performed as described previously [23 (link)], with minor modifications. Briefly, microtiter plates (Thermo Scientific Pierce 96-well polystyrene plates) were coated with 100 μl of AcrA, O157-AcrA or O145-AcrA (125 ng/well) in coating buffer (0.05 M carbonate buffer pH 9.6) for 18 h at 4°C. The plates were blocked in blocking buffer (5% bovine skim milk in TBS) for 1 h at 37°C and subsequently incubated with the indicated hybridoma supernatant or mice sera dilution for 1 h at RT in blocking buffer. Following four washing steps in TBS Tween-20 0.05%, plates were further incubated for 1 h at RT with HRP goat anti-mouse IgG secondary antibody (Sigma-Aldrich) at a 1:6000 dilution in blocking buffer. Finally, plates were washed four times in TBS 0.05% Tween-20 and after incubation with the substrate (0.3% H2O2, 0.1% 3,3',5,5'- tetramethylbenzidine [TMB] in 0.1 M citric acid pH 5) for 5 to 20 min at RT, the reaction was stopped with 0.2 M H2SO4. The absorbance at 450 nm was measured with a FilterMax F5 Multi-Mode microplate reader (Molecular Devices).
Castillo D.S., Rey Serantes D.A., Melli L.J., Ciocchini A.E., Ugalde J.E., Comerci D.J, & Cassola A. (2017). A recombinant O-polysaccharide-protein conjugate approach to develop highly specific monoclonal antibodies to Shiga toxin-producing Escherichia coli O157 and O145 serogroups. PLoS ONE, 12(10), e0182452.
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10

HTLV-1 LTR Transactivation Assay

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HEK293T cells were transfected using Mirus TransIT®-2020 transfection reagent (Mirus Bio LLC, Madison, WI) according to manufacturer’s instructions. HEK293T cells were co-transfected with 1 μg of HTLV-1, HTLV-1∆CTCF, HTLV-1 p12Stop, or empty (pcDNA3) plasmids along with 100 ng of LTR-1-Luc and 20 ng of TK-Renilla reporter plasmids. An HTLV-1 p19 Gag enzyme-linked immunosorbent assay (ELISA; Zeptometrix Corporation, Buffalo, NY) was performed with supernatant collected 48 h post-transfection. Transfected cells were also harvested at the time of supernatant collection. Cell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) [35 (link)]. Assays were performed with LTR-1-luc activity normalized for transfection efficiency using Renilla luciferase.
Martinez M.P., Cheng X., Joseph A., Al-Saleem J., Panfil A.R., Palettas M., Dirksen W.P., Ratner L, & Green P.L. (2019). HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo. Retrovirology, 16, 44.
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