Exfo x cite 120

Image acquisition and processing was performed as described previously61 (link). Cells were analysed with a microscope (Axiovert 200 M; Carl Zeiss MicroImaging, Inc.) equipped with an Exfo X-cite 120 excitation light source, band pass filters (Carl Zeiss MicroImaging, Inc. and Chroma Technology Corp.), an α Plan-Fluar 100x/1.45 NA, Plan-Apochromat 63x/1.4 NA or A-plan (Carl Zeiss MicroImaging, Inc.), and a digital camera (Orca ER; Hamamatsu). Image acquisition was performed using Volocity software (Improvision). Fluorescence images were collected as 0.5 μm Z-stacks using exposures of up to 200 ms, merged into one plane in Openlab, and processed further in Photoshop (Adobe). Brightfield images were collected in one plane, and processed where necessary to highlight circumference of the cells.

Cells were analysed with a microscope (Axiovert 200 M; Carl Zeiss, Oberkochen, Germany) equipped with an Exfo X-cite 120 excitation light source, band pass filters (Carl Zeiss and Chroma Technology, Rockingham, VT, USA), an α Plan-Fluar 100 × 1.45 NA and Plan-Apochromat 63 × 1.4 NA objective lens (Carl Zeiss), and a digital camera (Orca ER; Hamamatsu Photonics, Hamamatsu City, Japan). Image acquisition was performed using Volocity software version 7.0.0 (PerkinElmer, Waltham, MA, USA). Fluorescence images were collected as 0.5 μm z-stacks, merged into one plane using Openlab software version 5.5.2 (PerkinElmer), and processed further in Photoshop (Adobe, version 24.6). Bright-field images were collected in one plane and processed where necessary to highlight the circumference of the cells in blue. Each imaging experiment was performed at least three times, and representative images are shown. For quantitation analysis, 1–3 experiments were used. The graphs for quantitation analysis were plotted using GraphPad Prism 9 (GraphPad software, San Diego, CA, USA. www.graphpad.com accessed on 23 December 2022).

Cells were analyzed with a microscope (Axiovert 200M; Carl Zeiss) equipped with Exfo X-cite 120 excitation light source, band-pass filters (Carl Zeiss and Chroma), and a Plan-Fluor 100×/1.45 NA or Plan-Apochromat 63× 1.4 NA objective lens (Carl Zeiss) and a digital camera (Orca ER; Hamamatsu Phototonics). Image acquisition was performed using Volocity software (PerkinElmer). Fluorescence images were routinely collected as 0.5-µm z-stacks, merged into one plane in Openlab (PerkinElmer), and processed further in Photoshop (Adobe). Single focal planes are shown where indicated. Bright-field images were processed where necessary to highlight the circumference of cells in blue. For line-scan analyses, lines were drawn through a region of interest on ImageJ/FIJI to generate intensity profiles across the line for each channel.