Tumors were fixed with 10% neutral buffered formalin for 48 h, paraffin-embedded, and cut in 5-μm sections. Heat-induced antigen retrieval was performed in citrate buffer, and after blocking endogenous peroxidase, slides were incubated with primary antibody anti-PCNA antibody (PC10) (1:200 dilution, sc-56, Santa Cruz Biotechnology) as previously indicated [26 (link)]. Then, slides were incubated with a biotinylated antibody and developed with the ABC-HRP Kit (Vector Laboratories, Burlingame, CA, USA) and peroxidase substrate DAB (Sigma-Aldrich, St. Louis, MO, USA). Afterward, slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera. The PCNA cell count was quantified in four randomly selected fields from each animal and analyzed using ImageJ 1.54e software.
Axioplan microscope
Manufactured by Nikon
The Axioplan microscope is a high-quality optical instrument designed for advanced scientific and research applications. It features a stable and precise mechanical design, providing users with a reliable and consistent platform for their laboratory work. The Axioplan microscope offers a range of optical capabilities, including bright-field, phase-contrast, and fluorescence microscopy, allowing researchers to study a variety of samples in a controlled and versatile environment.
Automatically generated - may contain errors
Lab products found in correlation
Dxm1200f, by Nikon (5 mentions)
Sc 56, by Santa Cruz Biotechnology (2 mentions)
Abc hrp kit, by Vector Laboratories (1 mentions)
Sc 23900, by Santa Cruz Biotechnology (1 mentions)
Ab8158, by Abcam (1 mentions)
Sc 18917, by Santa Cruz Biotechnology (1 mentions)
Sc 74528, by Santa Cruz Biotechnology (1 mentions)
Eclipse e100 microscope, by Nikon (1 mentions)
Tunel label mix, by Roche (1 mentions)
Coolpix b500, by Nikon (1 mentions)
6 protocols using axioplan microscope
1
PCNA Immunohistochemistry Protocol
2
Immunohistochemical Analysis of Liver Tissue
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Livers were fixed and paraffin embedded. Sections were routinely stained with Hematoxylin&Eosin (7-μm) or incubated with mAb anti-PCNA antibody (PC10) (1:200 dilution, sc-56, Santa Cruz Biotechnology) as previously indicated [53 (link)]. The slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera. The PCNA cell count was quantified in four randomly selected fields from each animal and analyzed using ImageJ software. Ki-67 staining was performed using a specific antibody (sc-23900, 1:200 mouse) from Santa Cruz Biotechnology.
3
Histological Examination of Liver Tissue
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Livers were formalin-fixed and 7-µm sections were routinely stained with H&E following standard procedures. The slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera.
4
Liver Histology and Immunohistochemistry
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Livers were fixed and 7-μm sections were routinely stained with H&E or paraffin sections (5-μm) were prepared following standard procedures22 (link)23 (link)26 (link). The antibodies used were rat mAb anti-CD34 (1:50 dilution, ab8158, Abcam), goat polyclonal anti-antiogenin (C1) (1:200, sc-74528, Santa Cruz Biotechnology), mAb anti-PCNA antibody (PC10) (1:200 dilution, sc-56, Santa Cruz Biotechnology) and anti-CD34 (1:100 dilution, sc-18917, Santa Cruz Biotechnology). The slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera. PCNA index was quantified in four randomly selected fields from each animal, and CD34 positive areas analyzed using ImageJ software.
5
Apoptotic Cell Quantification in Liver Tissues
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Liver and tumor samples were fixed and 5-μm sections were prepared following standard procedures. The slices were examined with a Zeiss Axioplan microscope equipped with a Nikon DXM1200F digital camera. Apoptotic cells with fragmented nuclei were detected in paraffin samples using TUNEL labeling containing fluorescein-dUTP and -dNTPs (TUNEL Label Mix, Roche). TUNEL positive cells were observed and quantified using a NIKON Eclipse E-100 microscope.
6
Observing halophilic cell growth
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WCL brine samples were kept in the laboratory at room temperature and exposed to natural light. Cells were observed to slowly grow under these conditions. Subsamples of these brines were inoculated in a classical culture medium for halophiles (Rodriguez-Valera et al., 1980) slightly modi ed to match the pH and Mg concentrations of the WCLs (Tables S2-S3). Growth was monitored by optical microscopy using a Zeiss Axioplan microscope equipped with a Nikon Coolpix B500 color camera for image acquisition.
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