D luciferin beetle monosodium salt

Manufactured by Promega

Sourced in Germany

D-luciferin (beetle) monosodium salt is a biochemical reagent commonly used in bioluminescence assays. It serves as a substrate for the luciferase enzyme, which catalyzes a light-emitting reaction. This product is derived from the luciferin compound found in fireflies and other bioluminescent beetles.

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Lab products found in correlation

Penicillin, by Harvard Bioscience (3 mentions) Streptomycin, by Harvard Bioscience (3 mentions) Fbs superior, by Harvard Bioscience (3 mentions) Trypsin edta solution, by Harvard Bioscience (3 mentions) L glutamine, by Harvard Bioscience (3 mentions) Dulbecco s mem medium, by Harvard Bioscience (2 mentions) D glucose, by Avantor (2 mentions) Hepes, by Avantor (2 mentions) Sodium chloride (nacl), by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Avantor (2 mentions) Viafect transfection reagent, by Promega (1 mentions) Pluronic pe 10500, by BASF (1 mentions) R carvone, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Lactisole, by Cayman Chemical (1 mentions) Dulbecco s mem, by Merck Group (1 mentions) Probenecid, by Merck Group (1 mentions) Ly379268, by Bio-Techne (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) Pluronic f 127, by AAT Bioquest (1 mentions)

4 protocols using d luciferin beetle monosodium salt

Isomeric Purity and Aldehyde Screening

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Isomeric purity of (Z)-4-undecenal (Z4-11Al) was 98.6%, according to gas chromatography coupled to mass spectrometry (6890 GC and 5975 MS, Agilent Technologies, Santa Clara, CA, USA). Isomeric purity of (Z)-4-nonenal (Z4-9Al) and (Z)-6-undecenal (Z6-11Al) were 97.4 and 96%, repectively. Chemical purity of these synthetic aldehydes was >99.9%. Ethanol (redistilled; Merck, Darmstadt, Germany) was used as solvent.
For the OR screening assays, the following chemicals were used: Dulbecco’s MEM medium (#F0435), FBS superior (#S0615), L-glutamine (#K0282), penicillin (10000 U/ml)/streptomycin (10000 μg/mL) (#A2212), trypsin/EDTA solution (#L2143) (Biochrom, Berlin, Germany), CaCl2∗2H2O (#22322.295), D-glucose (#101174Y), dimethyl sulfoxide (DMSO) (#83673.230), HEPES (#441476L), potassium chloride (#26764.230), and sodium hydroxide (#28244.295) (VWR Chemicals BDH Prolabo, Leuven, Belgium), sodium chloride (#1064041000, Merck, Darmstadt, Germany), ViaFect™ Transfection Reagent (#E4981, Promega, Walldorf, Germany), D-luciferin (beetle) monosodium salt (#E464X, Promega, Walldorf, Germany), Pluronic® PE 10500 (#500053867, BASF, Ludwigshafen, Germany), (R)-(−)-carvone (#W224908, Sigma-Aldrich, Steinheim, Germany).

Frey T., Kwadha C.A., Haag F., Pelletier J., Wallin E.A., Holgersson E., Hedenström E., Bohman B., Bengtsson M., Becher P.G., Krautwurst D, & Witzgall P. (2022). The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogaster. iScience, 25(11), 105269.

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Calcium Signaling Assay for G-Protein Coupled Receptors

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The following chemicals were used: Dulbecco’s MEM, sodium chloride, 2-Mercaptoethanol (Merck KGaA, Darmstadt, Germany), FBS superior, L-glutamine, Penicillin (10,000 U/mL)/streptomycin (10,000 U/mL), Trypsin/EDTA solution (Bio & Sell GmbH, Feucht, Germany), Dimethyl sulfoxide (DMSO), HEPES, Potassium chloride, sodium hydroxide, calcium chloride dehydrate, D-glucose (VWR International GmbH, Darmstadt, Germany), D-luciferin (beetle) monosodium salt (Promega, Madison, WI, USA), Bordetella pertussis toxin, L-Glutamic acid monosodium salt monohydrate (Santa Cruz Biotechnology, Dallas, TX, USA), Forskolin (Biomol GmbH, Hamburg, Germany), Lactisole (Cayman Chemicals, Ann Arbor, MI, USA), fMLF (Tokio Chemical Industry, Tokyo, Japan), mGluR2 antagonist 1 (CAS: 1432728-49-8; Biozol GmbH, Eching, Germany), metabotropic glutamate receptor 2/3 agonist LY379268 (Tocris Bioscience, Bristiol, UK), Gibco RPMI 1640 containing L-glutamine (Thermo Fisher Scientific, Waltham, MA, USA), Probenecid (Sigma-Aldrich, St. Louis, MO, USA), Pluronic^® F-127 (AAT Bioquest Inc., Sunnyvale, CA, USA), Fluo-4 AM (Bio-Techne, Minneapolis, MN, USA). Calcium buffer was composed of 140 mM NaCl, 20 mM HEPES, 5 mM KCl, 1.8 mM CaCl₂, and 0.5 mM D-glucose, adjusted pH 7.4.

Ball L., Bauer J, & Krautwurst D. (2023). Heterodimerization of Chemoreceptors TAS1R3 and mGlu2 in Human Blood Leukocytes. International Journal of Molecular Sciences, 24(16), 12942.

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Odorant Receptor Activation Assay

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The following chemicals were used: Dulbecco′s
MEM medium (#F0435), FBS superior (#S0615), l-glutamine (#K0282),
penicillin (10 000U/mL)/streptomycin (10 000U/mL) (#A2212), trypsin/EDTA
solution (#L2143) (Biochrom, Berlin, Germany), calcium chloride dihydrate
(#22322.295), d-glucose (#101174Y), DMSO (#83673.230), HEPES
(#441476L), potassium chloride (#26764.230), and sodium hydroxide
(#28244.295) (VWR Chemicals BDH Prolabo, Leuven, Belgium), sodium
chloride (#1064041000, Merck, Darmstadt, Germany), D-luciferin (beetle)
monosodium salt (#E464X, Promega, Madison, USA). The following odorants
were used: 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN; CAS: 30364–38–6)
(#ENAH97EDDC8E), 2-acetylnaphthalene (CAS: 93–08–3)
(#134775), and methyl isoeugenol (CAS: 93–16–3) (#W247618,
Sigma-Aldrich, Darmstadt, Germany). The KFOs used were as previously
published^{39 (link),40 (link)} (Table S1). Further
details including the GC–O approach for the purity testing
of the odorants prior to their use in the odorant receptor experiments
are available in the literature.^{41 (link)}

Haag F., Frey T., Ball L., Hoffmann S, & Krautwurst D. (2024). Petrol Note in Riesling – 1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) Selectively Activates Human Odorant Receptor OR8H1. Journal of Agricultural and Food Chemistry, 72(9), 4888-4896.

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Pyrazine-based Odor Compound Screening

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Dulbecco´s MEM (#F0435), FBS superior (#S0615), Lglutamine (#K0282), penicillin (10 000 U/mL)/streptomycin (10 000 U/mL) (#A2212), trypsin/EDTA solution (#L2143) (formerly Biochrom, Berlin, Germany, now Merck KGaA), calcium chloride dehydrate (#22322.295), D-glucose (#101174Y), dimethyl sulfoxide (DMSO) (#83673.230), HEPES (#441476L), potassium chloride (#26764.230), sodium hydroxide (#28244.295) (VWR International GmbH, Darmstadt, Germany), sodium chloride (#1064041000, Merck KGaA, Darmstadt, Germany), and D-luciferin (beetle) monosodium salt (#E464X, Promega, Madison, USA), 2,5-dihydro-2,4,5-trimethylthiazoline (CAS# 4145-93-1) (#AB494350, abcr GmbH, Karlsruhe, Germany).
Pyrazines and other odorants are listed in Table S1. Compounds of particular interest for our experiments are listed alongside their assigned compound numbers, CAS numbers, structures, acronyms, and designation as KFO and/ or semiochemical in Table 1. Compounds utilized in the KFO screen were as previously published 38, 39 (Table S2).

Marcinek P., Haag F., Geithe C, & Krautwurst D. (2021). An evolutionary conserved olfactory receptor for foodborne and semiochemical alkylpyrazines. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(6).

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