All procedures and experiments were performed according to the guidelines of the Canadian Council on Animal Care and approved by the animal Care Committee of the Montreal Children's Hospital. WT CD1, mT/mG Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J, (Soriano, 1999 (link)), Wnt1-Cre2 (Lewis et al., 2013 (link)) and β-actin-Cre (Lewandoski et al., 1997 (link)) mice on the C57BL/6J genetic background were purchased from The Jackson Laboratory. The R26R strain [Gt (ROSA)26Sortm1Sor (Muzumdar et al., 2007 (link))] on the mix C57BL/6J;129/S4 genetic background was a kind gift from Dr Nagano (Department of Obstetrics and Gynecology, McGill University, Montreal, Canada). All strains were maintained on the CD1 genetic background. The Trp53tm1brn mouse line with loxP sites flanking exons 2-10 of the Trp53 gene was purchased from The Jackson Laboratory (Trp53loxP/+) (stock #008462 ) (Marino et al., 2000 (link)).
Trp53loxp
Manufactured by Jackson ImmunoResearch
The Trp53loxP/+ is a laboratory mouse strain that carries a loxP-flanked (floxed) Trp53 allele. Trp53 is the gene that encodes the p53 protein, a key regulator of cellular processes. This mouse model allows for the conditional deletion or modification of the Trp53 gene in a cell-type or tissue-specific manner using Cre recombinase technology.
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Lab products found in correlation
Wnt1 cre2, by Jackson ImmunoResearch (1 mentions)
β actincre, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Lsl krasg12d, by Jackson ImmunoResearch (1 mentions)
R26 tdtomato, by Jackson ImmunoResearch (1 mentions)
Corn oil, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
2 protocols using trp53loxp
1
Genetic Mouse Strains for Research
2
Conditional Genetic Manipulation in Mice
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Mice were maintained in the specific pathogen-free (SPF) facility of KAIST Laboratory Animal Resource Center and housed under a 12h/12h light/dark cycle, with free access to regular chow and water. All mice were grouped by no more than 5 mice per cage. Mouse experiments were performed with the approval of the IACUC of Korea Advanced Institute of Science and Technology (KAIST) (KA2020-115). LSL-K-rasG12D, Trp53LoxP, and R26-tdTomato mice were purchased from The Jackson Laboratory and described previously.49 (link),50 (link),51 (link)
Prmt1fl/fl mice (Mouse Genome Informatics [MGI]:4432476) were purchased from the International Mouse Phenotyping Consortium (IMPC) and described previously.13 (link)
Pdx1-CreERT2 mice were provided by Dr. Douglas A. Melton (Harvard University) and described previously.52 (link) Inducible Cre-mediated gene deletion was achieved by i.p. injection of tamoxifen (Sigma) at a dose of 200 mg/kg bodyweight on three alternate days at 3 to 4 weeks of age. tamoxifen was dissolved in corn oil (Sigma) at a concentration of 20 mg/mL.
Prmt1fl/fl mice (Mouse Genome Informatics [MGI]:4432476) were purchased from the International Mouse Phenotyping Consortium (IMPC) and described previously.13 (link)
Pdx1-CreERT2 mice were provided by Dr. Douglas A. Melton (Harvard University) and described previously.52 (link) Inducible Cre-mediated gene deletion was achieved by i.p. injection of tamoxifen (Sigma) at a dose of 200 mg/kg bodyweight on three alternate days at 3 to 4 weeks of age. tamoxifen was dissolved in corn oil (Sigma) at a concentration of 20 mg/mL.
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