The SkyScan 1172 system (Bruker micro-CT, Kontich, Belgium) was used for the CT scanning of chick phalanges as previously described with some modification [52 (link)]. For quantitative analysis, 1000 horizontal sections in total were selected to form OZ, namely selecting 500 horizontal sections on both sides of OZ, and trabecular bone regions were manually delineated adjacent to the trabecular bone outline. Image acquisition parameters used were: 5 µm voxel resolution, 0.5 mm aluminum filter, 80 kV voltage and 100 μA current, 750 ms exposure time, 0.4° rotation step, and using frame averaging. Reconstruction and analysis of images was performed using SkyScan software (Bruker micro-CT, Kontich, Belgium), NRecon (1.6.9.8), DataViewer (1.5.0), CTAnalyser (1.13.11.0) and CTvol (2.3.2.0).
Skyscan 1172 system
Manufactured by Bruker
Sourced in Belgium, United States
The SkyScan 1172 system is a high-resolution X-ray micro-CT scanner for non-destructive 3D imaging of a wide range of samples. It provides detailed, high-quality 3D images of the internal structure and composition of objects.
Automatically generated - may contain errors
Lab products found in correlation
Nrecon software, by Bruker (5 mentions)
Nrecon, by Bruker (4 mentions)
Ctvol software, by Bruker (4 mentions)
Ctvox software, by Bruker (2 mentions)
3d ctan software, by Bruker (2 mentions)
Ctan software, by Bruker (2 mentions)
Skyscan software, by Bruker (1 mentions)
Ct analyzer software, by Bruker (1 mentions)
Skyscan 1076 system, by Bruker (1 mentions)
Faxitron mx 20 specimen radiography system, by Hologic (1 mentions)
1272 system, by Bruker (1 mentions)
Mimics 14.0 imaging software, by Materialise (1 mentions)
Alpha t spectrometer, by Bruker (1 mentions)
Skyscan, by Bruker (1 mentions)
26 protocols using skyscan 1172 system
1
Chick Phalanges Micro-CT Imaging
2
Distal Femur Microarchitecture Analysis
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Distal femur µCT images were taken on the high resolution Skyscan 1172 system (Bruker, Kontich, Belgium). The scanner X-ray source was set at a voltage of 50 kV and a current of 200 µA and a 0.5 mm aluminum filter was applied. Scans were taken with a pixel size of 5 µm, the scanning angular rotation was 180° with an angular increment of 0.4° and with a frame averaging of 2. Serial tomographs were reconstructed from raw data with the cone-beam reconstruction software (NRecon software, Skyscan, Bruker) based on the Feldkamp algorithm [16] . 3D morphometric analysis of reconstructed datasets was performed using the CT Analyzer software (CTAn, Bruker). Cortical and trabecular volumes of interest were manually selected [2 -2.5 mm (cortical) and 0.5 -1.5 mm (trabecular) under the selected reference point (point were trabecular bone is fully separated into four compartments on a transverse plane)]. Cortical and trabecular regions of interest were selected using the "automated trabecular and cortical bone selection method" (Method note, Bruker). Binary images for analysis were generated using a global thresholding of 80-255 (cortical) or 75-255 (trabecular). 3D models were constructed with the CTvox software (Bruker) using different selections [2.35 -2.5 mm (cortical) and 0.25 -2.75 mm (trabecular) under the same reference point].
3
Microstructural Analysis of Femoral Bone
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The femurs were dissected after the peptides’ injection, and three-dimensional Micro CT analysis was performed using a Skyscan 1172 system (Bruker, Kontich, Belgium) at 55 kV (167 µA) and a 10 µm voxel size, as described.66 (link),67 (link) The skeletal parameters were assessed by micro-CT and followed published nomenclature guidelines.68 (link) Trabecular bone microarchitecture [trabecular bone volume fraction (BV/TV), trabecular bone thickness (Tb. Th), trabecular number (Tb. N), and trabecular separation (Tb. Sp)] was analyzed in a manually delineated region of interest 0.25–2.5 mm proximal to the distal femoral growth plate. Cortical thickness (Cs. Th) was 0.6 mm region at the femoral mid-diaphysis.
4
High-Resolution Micro-CT Analysis of Tibiae
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MicroCT analysis of the tibiae was performed ex vivo using the high resolution SkyScan 1172 system (Bruker; 50 kV, 200 μA, 0.5 mm aluminium filter) and in vivo using the SkyScan 1076 system (Bruker; 50 kV, 100 µA, 1 mm aluminium filter)55 (link). Serial tomographs, reconstructed from raw data using the cone-beam reconstruction software (NRecon; Bruker), were used to compute trabecular and cortical parameters, respectively from the metaphyseal and mid-diaphyseal area. All measurements were performed according to the guidelines of the American Society for Bone and Mineral Research56 (link).
5
Micro-CT Analysis of Fixed Samples
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Before the decalcification for histological processing, samples fixed in 10% buffered formalin for 36 h, were submitted to micro-computed tomography (micro-CT) for morphometric analysis using the SkyScan 1172 system (Bruker-SkyScan, Kontich, Belgium). Images were acquired at 60 kVp and 200 mA and reconstructed using the software NRecon (Bruker-Skyscan) with smoothing 1, ring artifact correction 5 and beam hardening correction 20%. The micro-CT analyses were carried out using the 3D Ctan software (BruKer-Skyscan) to evaluate bone volume, percentage of bone volume, bone surface and trabecular number, thickness and separation.
6
Micro-CT Analysis of Distal Femurs
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Micro-computed tomography (µCT) was performed on distal femurs from age- and sex-matched mice with the Skyscan 1172 system (Bruker, Billerica, MA, USA) as described previously [41 (link)]. Bone parameters were calculated using CTAn software (Bruker). 3D models of bones were generated using CTVol software (Bruker).
7
Femoral Bone Structure Analysis
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Right femurs were scanned on a SkyScan 1172 system (Bruker, Billerica, MA, USA) with a 0.5 aluminum filter and a 6 μm voxel size. Trabecular bone was analyzed in a 1 mm region starting proximal to the growth plate in the distal femur. Cortical bone properties were analyzed on 5 contiguous slices located ~2.5 mm proximal to growth plate in the distal femur. Cortical porosity was determined by assessing void area between the periosteal and endosteal surfaces, presented as a % of overall cortical volume as previously described [15 (link), 16 (link)].
8
Bone Morphometry Analysis of Murine Femurs
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Radiographic images of each limb and cranium were obtained by Faxitron MX-20 Specimen Radiography System (Faxitron X-ray Corp.) at an energy of 30 kV for 90 seconds with Eastman X-OMAT TL film (Eastman Kodak Co.) and processed by an automated X-ray film developer (Model M35A, Eastman Kodak Co.). Bone thickness was measured between the outer edges of cortical bone at the mid-diaphysis using ImageJ software (free from the NIH website).
Femurs from 20-weeks-old mice (n = 3 male mice/group) were scanned, using a SkyScan 1172 System (Bruker) with a source voltage and current of 65 kV and 153 μA, respectively. Following scanning, three-dimensional microstructural images were reconstructed using SkyScanNRecon software. SkyScan CT Analyzer software was used to calculate cortical micro-architectural parameters: cortical thickness (Ct.Th/mm), Total Area (TA/mm2), Bone Area (BA/mm2), and Medullary Area (MA/mm2).
Femurs from 20-weeks-old mice (n = 3 male mice/group) were scanned, using a SkyScan 1172 System (Bruker) with a source voltage and current of 65 kV and 153 μA, respectively. Following scanning, three-dimensional microstructural images were reconstructed using SkyScanNRecon software. SkyScan CT Analyzer software was used to calculate cortical micro-architectural parameters: cortical thickness (Ct.Th/mm), Total Area (TA/mm2), Bone Area (BA/mm2), and Medullary Area (MA/mm2).
9
Quantitative Femoral Bone Microstructure
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Micro-computed tomography (μCT) was performed on distal femurs with the Skyscan 1172 system (Bruker), as described previously.19 (link) 3D models of bones were generated using CTVol software (Bruker). The number of cortical perforations > 50 μm in diameter was counted blinded on reconstructed images.
10
I2KI Enhanced microCT Imaging Protocol
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In preparation for I2KI enhanced microCT scanning, one C57BL/6 mouse (female, body mass 24.9g, age 117 days) was euthanized by cervical dislocation, and its right hindlimb was removed, skinned and immediately frozen. The limb was then thawed and placed in 10% neutral-buffered formalin (NBF) (HT501128-4L, Sigma) for 24hrs at room temperature to fix the soft tissue, and subsequently placed in 1x phosphate buffered saline (PBS) to remove residual fixative. The specimen was then immersed in an aqueous solution of 15% iodine-potassium iodide (I2KI, Lugols solution, Sigma, L6146) for eight days to enhance soft tissue contrast, and then placed in 70% ethanol solution until microCT scanning. Scanning was carried out using a SkyScan 1172 system (Bruker microCT, Belgium) at the following settings: 12.96μm resolution, 70kV, 141μA, Al 0.5mm filter. The images were reconstructed using NRecon software (Bruker microCT, Belgium), whereas images of the internal structures of the hindlimb were captured using CTvox software (Bruker microCT, Belgium) (Fig 1 ).
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