Ix71 phase contrast microscope

Manufactured by Olympus

Sourced in Japan

The IX71 is a phase contrast microscope manufactured by Olympus. It is designed for high-quality imaging of unstained, transparent samples. The IX71 utilizes a phase contrast optical system to enhance the contrast of specimens, enabling detailed observation of cellular structures and other transparent materials.

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Lab products found in correlation

Guava easycyte, by Merck Group (1 mentions) Matrigel, by BD (1 mentions) Diaphot, by Nikon (1 mentions) Guxmx 90021, by Cyagen (1 mentions) Guxmx 90041, by Cyagen (1 mentions) Guxmx 90031, by Cyagen (1 mentions)

Close spelling variants of this product

IX71 microscope (1097 citations) Phase-contrast microscope (486 citations) IX71 inverted phase contrast microscope (10 citations) IX71 Inverted Fluorescence Phase Contrast Microscope (4 citations)

9 protocols using ix71 phase contrast microscope

Sperm Motility Quantification via High-Speed Videography

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Sperm were video-recorded on a 37°C-warm plate at 200 frames per sec under an IX-71 phase-contrast microscope (Olympus) equipped with an HAS-220 high-speed camera (DITECT, Tokyo, Japan).
Trajectories of sperm were analyzed using the Manual Tracking plugin of ImageJ software (http://rsbweb.nih.gov/ij/). The apical end of sperm head was tracked at 200 frames per sec for 1 sec. Parameters of sperm motility were quantified by the coordinates of sperm
head for 1 sec. The following parameters were assessed, as described [18 (link), 19 (link)]: curvilinear velocity (VCL,
μm/sec) calculated by the sum of the distances along the trajectory for 1 sec; average path velocity (VAP, μm/sec), where the average path is calculated by averaging coordinates from
one-sixth (33 frames) of video-frame rates; straight-line velocity (VSL, μm/sec) calculated by the straight-line distance between the first and last points of the trajectory for 1 sec;
linearity (LIN, %) calculated by dividing VSL by VCL; straightness (STR, %) calculated by dividing VSL by VAP; wobble (WOB, %) calculated by dividing VAP by VCL.

NAGASHIMA K., USUI T, & BABA T. (2018). Behavior of ACRBP-deficient mouse sperm in the female reproductive tract. The Journal of Reproduction and Development, 65(2), 97-102.

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Evaluating Cell Morphology and Viability

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Cells were seeded in 6 cm flat-bottom culture plates (180,000 cells in 3 mL of culture medium). After 24 h, the cells were treated with 5 μM MHPT or DMSO. Morphological changes in the cells were observed, and the cells were photographed at 6, 12, 24 and 48 h by using an Olympus IX 71 phase-contrast microscope (Center Valley, PA, USA). Then, these cells were harvested and stained with Guava Viacount reagent for counting viable cells [14 ]. The stained cell samples were analyzed by flow cytometry (Guava EasyCyte, Millipore, USA).

Mu Y., Liu Y., Li L., Tian C., Zhou H., Zhang Q, & Yan B. (2015). The Novel Tubulin Polymerization Inhibitor MHPT Exhibits Selective Anti-Tumor Activity against Rhabdomyosarcoma In Vitro and In Vivo. PLoS ONE, 10(3), e0121806.

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Image Analysis and Microscopy Protocol

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An IX71 phase contrast microscope (Olympus, Tokyo, Japan) and QCapture Suite software (QImaging, Surrey, BC) were used for image analysis. A confocal microscope, SP8 (Leica, Wetzlar, Germany), was used for immunocytochemical analyses.

Gazarian K., Ramirez-Garcia L., Tapía Orozco L., Luna-Muñoz J, & Pacheco-Herrero M. (2021). Human Dental Pulp Stem Cells Display a Potential for Modeling Alzheimer Disease-Related Tau Modifications. Frontiers in Neurology, 11, 612657.

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Morphological Characterization of SHED

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Morphological characterization of SHED was performed using an Olympus IX71 phase contrast microscope. Image analysis was performed using QCapture Suite software.

Gazarian K.G, & Ramírez-García L.R. (2017). Human Deciduous Teeth Stem Cells (SHED) Display Neural Crest Signature Characters. PLoS ONE, 12(1), e0170321.

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Cell Migration and Invasion Assay

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Cell migration and invasion assays were performed using a 24-well chemotaxis chamber (Cell culture companion plates #353504 and 8.0-µm inserts #352097, BD Transduction Laboratories) as previously described^{40 (link)}. For the invasion assay, each well was coated with 100 µl of 1.6 mg/ml Matrigel (#354234, BD Transduction Laboratories). The cells (1 × 10⁵ cells in 200 µl serum-free medium) were inoculated into the upper insert, and 750 µl of growth medium was placed in the lower chamber as a chemo-attractant. For the inhibition assay, the cells were incubated with anti-αvβ3 integrin function blocking antibody (23C6, 1:100) for 20 min at room temperature before plating. After incubation for 6 h at 37 °C in a 5% CO₂ incubator, the cells were briefly washed twice with PBS, fixed with 4% paraformaldehyde for 10 min and stained with 0.5% crystal violet in 20% methanol. The cells on the upper side of the membrane and the Matrigel were removed with cotton swabs. Three randomly chosen fields were photographed using an IX71 phase contrast microscope (Olympus), and the migrated cells were counted.

Kariya Y., Oyama M., Suzuki T, & Kariya Y. (2021). αvβ3 Integrin induces partial EMT independent of TGF-β signaling. Communications Biology, 4, 490.

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Scratch Wound Healing Assay

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The cells were cultivated for 96 h with or without treatment with siAHR/siELAVL1/GEM. After treatment, the cells were detached by trypsin/EDTA, counted, and seeded into 24-well culture plates at concentration of 2 × 10⁵ cells/well. After 24 h, a scratch was made with a 200 µL pipette tip, and the medium was changed into a fresh medium without FBS. The scratch was observed and photographed under an Olympus IX71 phase-contrast microscope at 0 and 24 h after making the scratch. No less than 3 replicates of the experiment were carried out.

Stukas D., Jasukaitiene A., Bartkeviciene A., Matthews J., Maimets T., Teino I., Jaudzems K., Gulbinas A, & Dambrauskas Z. (2023). Targeting AHR Increases Pancreatic Cancer Cell Sensitivity to Gemcitabine through the ELAVL1-DCK Pathway. International Journal of Molecular Sciences, 24(17), 13155.

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Assessment of Sperm Hyperactivation

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An aliquot of 150 μL of BWW medium was placed in a prewarmed culture dish (35 × 10 mm, Corning, New York, USA) and covered with a prewarmed 22 × 22 mm coverslip. An aliquot of 20 μL sperm sample was added to a corner of the coverslip. The culture dish was placed in an inverted microscope (Diaphot, Nikon, London, UK) fitted with a thermostatically controlled air heated cabinet (Nikon, London, UK) equilibrated at 37°C. Sperm capacitation status was assessed based on hyperactivation by the objective photographic method using an Olympus IX71 phase-contrast microscope. Since hyperactivation is a flagella phenomenon, it is possible to visually assess hyperactivation based on sperm motility; flagella movement patterns were evaluated for the enumeration of nonhyperactivated and hyperactivated sperm [17 (link)]. Experiments were performed in duplicate and repeated independently four times.

Dhanushka M.A, & Peiris L.D. (2017). Cytotoxic and Genotoxic Effects of Acephate on Human Sperm. Journal of Toxicology, 2017, 3874817.

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Evaluating Pancreatic Cancer Cell Colony Formation

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The colony formation of pancreatic cancer cells was evaluated using a crystal violet stain. The cells were cultivated for 96 h with or without treatment with siAHR/siELAVL1/GEM. After treatment, the cells were detached by trypsin/EDTA, counted, and seeded into 6-well culture plates at concentration of 600 cells/well. After 7 days of growth, formed colonies were fixed with 96% ethanol and stained with crystal violet stain. After staining, crystal violet was removed, and the wells were rinsed with water. Plates were dried at room temperature, the morphology of cells was observed, and colonies were counted under an Olympus IX71 phase-contrast microscope (Olympus Corporation, Tokyo, Japan). No less than 4 replicates of the experiment were carried out.

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Adipogenic, Osteogenic, and Chondrogenic Differentiation Assays

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In the adipogenesis or osteogenesis assay, cells were, respectively, cultured in Human Mesenchymal Stem Adipogenic Differentiation Medium (supplemented with insulin, dexamethasone, 3-isobutyl-1-methylxanthine and indometacin) (GUXMX-90,031, Cyagen Biosciences, USA) or Osteogenic Differentiation Medium (supplemented with dexamethasone, b-glycerophosphate and L-ascorbic acid-2-phosphate) (GUXMX-90,021, ditto for supplier) for 2 weeks. Then the cells were fixed with 4% paraformaldehyde and stained with Oil Red O or Alizarin Red according to the manufacturers’ instructions.
For the chondrogenesis assay, cells were transferred into a 15-ml centrifuge tube and were centrifuged for 4 min at 250 g at room temperature. Then, the intact cell pellet was carefully transferred into Human Mesenchymal Stem Chondrogenic Differentiation Medium (supplemented with dexamethasone, ascorbate, insulin-transferrin-selenium supplement, sodium pyruvate, proline, TGF-β3) (GUXMX-90,041, Cyagen Biosciences, USA). After 3 weeks, the firm cell spheroid formed was fixed and sectioned, followed by Alcian Blue staining. The samples were imaged under an OLYMPUS IX71 phase contrast microscope (Olympus Corporation, JPN).

Qian Y., Chen H., Pan T., Li T., Zhang Z., Lv X., Wang J., Ji Z., He Y., Li L, & Lin M. (2021). Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway. Adipocyte, 10(1), 174-188.

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