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Anti pe and anti apc microbeads

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Anti-PE (phycoerythrin) and anti-APC (allophycocyanin) microbeads are small magnetic particles coated with antibodies that bind specifically to the fluorescent dyes PE and APC, respectively. These microbeads are used in flow cytometry and cell separation applications to detect and isolate cells labeled with these fluorescent markers.

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Lab products found in correlation

Collagenase p digestion, by Roche (2 mentions) Lsrii fortessa, by BD (1 mentions) Anti cd3 bv510, by BioLegend (1 mentions) Anti cd45ra fitc, by BD (1 mentions) Anti cd8 percp cy5, by BD (1 mentions) Anti cd4 bv650, by BD (1 mentions) Anti cd14 apc h7, by BD (1 mentions) Anti cd71 bv421, by BD (1 mentions) Anti cd27 bv711, by BD (1 mentions) Anti ccr7 af700, by BD (1 mentions) Anti hla dr bv605, by BioLegend (1 mentions) Anti cd19 apc h7, by BD (1 mentions) Anti cd38 bv786, by BD (1 mentions) Anti pd 1 pe cy7, by BD (1 mentions) Magnetized column, by Miltenyi Biotec (1 mentions) Lsr fortessa cytometer, by BD (1 mentions) Magnetized ls column, by Miltenyi Biotec (1 mentions)

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Anti-PE microbeads (357 citations) Anti-APC microbeads (112 citations)

5 protocols using anti pe and anti apc microbeads

1

Detecting Insulin-Specific CD4+ T Cells

Cited 2 times
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Insulin-specific CD4+ T cells were detected using insB10-23r3:I-Ag7 tetramer reagent and dual color staining with magnetic enrichment as described [24 (link), 25 (link)]. Briefly, single cell suspensions were incubated for one hour at room temperature with 10 nM of tetramers conjugated to phycoerythrin (PE) and allophycocyanin (APC) in medium containing Fc receptor block (2.4G2) and 0.05% sodium azide. Spleen and non-draining lymph node samples were subjected to magnetic enrichment following a 30 minute incubation with both anti-PE and anti-APC microbeads at 4°C (Miltenyi Biotec). Single cell suspensions from the pancreas were isolated using collagenase P digestion (Roche) and discontinuous Percoll gradients (44%/67%) [24 (link), 25 (link)]. Following tetramer staining, single cell suspensions from pancreas and pancreatic lymph node were subjected to surface staining for flow cytometric analysis.
Martinov T., Spanier J.A., Pauken K.E, & Fife B.T. (2016). PD-1 pathway-mediated regulation of islet-specific CD4+ T cell subsets in autoimmune diabetes. Immunoendocrinology (Houston, Tex.), 3, e1164.
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2

Detecting Insulin-Specific CD4+ T Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Insulin-specific CD4+ T cells were detected using insB10-23r3:I-Ag7 tetramer reagent and dual color staining with magnetic enrichment as described [24 (link), 25 (link)]. Briefly, single cell suspensions were incubated for one hour at room temperature with 10 nM of tetramers conjugated to phycoerythrin (PE) and allophycocyanin (APC) in medium containing Fc receptor block (2.4G2) and 0.05% sodium azide. Spleen and non-draining lymph node samples were subjected to magnetic enrichment following a 30 minute incubation with both anti-PE and anti-APC microbeads at 4°C (Miltenyi Biotec). Single cell suspensions from the pancreas were isolated using collagenase P digestion (Roche) and discontinuous Percoll gradients (44%/67%) [24 (link), 25 (link)]. Following tetramer staining, single cell suspensions from pancreas and pancreatic lymph node were subjected to surface staining for flow cytometric analysis.
Martinov T., Spanier J.A., Pauken K.E, & Fife B.T. (2016). PD-1 pathway-mediated regulation of islet-specific CD4+ T cell subsets in autoimmune diabetes. Immunoendocrinology (Houston, Tex.), 3, e1164.
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3

Tetramer-Based Enrichment of Antigen-Specific T Cells

Cited 1 time
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PBMCs (2.4–10 × 106) were stained with WT HLA-A2S269–277-PE and variant HLA-A2S269–277-APC tetramers at room temperature for 1 h in MACS buffer (PBS with 0.5% BSA and 2 mM EDTA). Cells were then incubated with anti-PE and anti-APC microbeads (Miltenyi Biotec), and tetramer+ cells were enriched using magnetic separation (9 (link)). Lymphocytes were stained with anti-CD71-BV421 (#562995), anti-CD4-BV650 (#563875), anti-CD27-BV711 (#563167), anti-CD38-BV786 (#563964), anti-CCR7-AF700 (#561143), anti-CD14-APC-H7 (#560180), anti-CD19-APC-H7 (#560177), anti-CD45RA-FITC (#555488), anti-CD8-PerCP-Cy5.5 (#565310), anti-CD95-PE-CF594 (#562395), anti-PD1-PE-Cy7 (#561272) (BD Biosciences), anti-CD3-BV510 (#317332), anti-HLA-DR-BV605 (#307640) (BioLegend), and LIVE/DEAD near-infrared stain (#L10119, Invitrogen) for 30 min, fixed with 1% PFA before acquiring data on an LSRII Fortessa (BD Bioscience). FCS files were analyzed using FlowJo v10 software.
Chaurasia P., Nguyen T.H., Rowntree L.C., Juno J.A., Wheatley A.K., Kent S.J., Kedzierska K., Rossjohn J, & Petersen J. (2021). Structural basis of biased T cell receptor recognition of an immunodominant HLA-A2 epitope of the SARS-CoV-2 spike protein. The Journal of Biological Chemistry, 297(3), 101065.
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4

Tet-labeled Cell Enrichment Protocol

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Tet–labeled cells were enriched using anti-PE and anti-APC microbeads and magnetized columns (Miltenyi Biotech) as described previously (33 (link)). For details, see SI Appendix.
Hassler T., Urmann E., Teschner S., Federle C., Dileepan T., Schober K., Jenkins M.K., Busch D.H., Hinterberger M, & Klein L. (2019). Inventories of naive and tolerant mouse CD4 T cell repertoires reveal a hierarchy of deleted and diverted T cell receptors. Proceedings of the National Academy of Sciences of the United States of America, 116(37), 18537-18543.
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5

Quantifying MCMV-specific CD8+ T Cells

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To determine the endogenous naive precursor frequency of MCMV-specific CD8+ T cell populations in C57BL/6 and BALB/c mice, enrichment assays of antigen-specific CD8+ T cells were performed as described [42 (link)]. In short, single cell suspensions were generated from pooled spleen and lymph nodes (mesenteric, inguinal, cervical, axillary, and brachial) of individual mice. Cells were stained with PE and APC-labelled MHC class I tetramers for 0.5 h at RT, then washed, labelled with anti-PE and anti-APC microbeads (Miltenyi Biotec), and passed over a magnetized LS column (Miltenyi Biotec). The tetramer-enriched fractions were stained with fluorochrome labelled Abs against CD3 (clone 500A2), CD4 (clone L3T4), CD8 (clone 53–6.7) for 30 min at 4°C, and subsequently analysed. Samples were acquired with the LSRFortessa cytometer (BD Biosciences).
Panagioti E., Redeker A., van Duikeren S., Franken K.L., Drijfhout J.W., van der Burg S.H, & Arens R. (2016). The Breadth of Synthetic Long Peptide Vaccine-Induced CD8+ T Cell Responses Determines the Efficacy against Mouse Cytomegalovirus Infection. PLoS Pathogens, 12(9), e1005895.
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