Anti actin clone ac 40

All chemicals were sourced from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated. For the western blot analysis, the following primary and secondary antibodies were used: Anti-cleaved caspase-6 (#9761), anti-cleaved caspase-7 (#9491), anti-cleaved caspase-8 (#9496), anti-cleaved caspase-9 (#9505), anti-PARP (#9542), anti-ATF6 (#65880), anti-BiP (#3177), anti-CHOP (#2895), anti-EGFR (#4267), and anti-MEK (#8727) from Cell Signaling Technology (Danvers, MA, USA). Anti-H-FABP (ab45966) and anti-CD36 (ab133625) from Abcam (Cambridge, UK), and anti-actin (clone AC-40).

All reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified. The following primary and secondary antibodies were used to detect protein expression: anti-CPS1 (B1) (sc376190, 1:500) from Santa-Cruz Biotechnology (Santa Cruz, CA, USA), anti-actin (clone AC-40, dilution 1:1000), anti-CPS1 [EPR7493-3] (ab129076, 1:1000), anti-GAPDH (ab9485, 1:1000) from Abcam (Cambridge, UK), anti-SDHA (D6J9M) XP® (#11998, 1:1000), anti-Smac/Diablo (79-1-83) (#2954, 1:1000), anti-COX IV (#11967S, 1:200), anti-Hop (D10E2) (#5670, 1:1000), anti-Calnexin (C5C9) (#2679, 1:1000) from Cell Signaling Technology (Danvers, MA, USA). Secondary antibodies HRP-linked goat anti-rabbit (sc-2004, 1:5000) or goat anti-mouse (sc-2005, 1:5000) were from Santa-Cruz Biotechnology (Santa Cruz, CA, USA). Secondary goat anti-rabbit IgG H&L Alexa Fluor®488 (ab150077) was from Abcam (Cambridge, UK), and goat anti-mouse IgG H&L F(ab)₂ Alexa Fluor®594 (#8890S) was from Cell Signaling Technology (Danvers, MA, USA).

Chemicals were from Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated. The following primary and secondary antibodies for Western blot analysis were used: anti-phospho-MKK3/6 (#9236), anti-p38 MAPK (#8690), anti-phospho-p38 MAPK (#4511), anti-phospho-MAPKAPK-2 (#3007), anti-phospho-c-Raf (#9427), anti-phospho-MEK1/2 (#9154), anti-ERK1/2 (#5013), anti-phospho-ERK1/2 (#4370), anti-BiP (#3177), anti-CHOP (#2895), anti-eIF2α (#9722), anti-phospho-eIF2α (#9261), anti-IRE1α (#3294), anti-SAPK/JNK (#9258), anti-phospho-SAPK/JNK (#4668), anti-c-Jun (#9165) and anti-phospho-c-Jun (#9261), anti-PARP (#9542), anti-cleaved caspase-7 (#9491), anti-cleaved caspase-8 (#9496), anti-cleaved caspase-9 (#9505) from Cell Signaling Technology (Danvers, MA, USA), anti-phospho-IRE1α (ab 48187) and anti-GAPDH (ab9485) from Abcam (Cambridge, UK), and anti-actin (clone AC-40).