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Rabbit anti gfp

Manufactured by GeneTex
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Rabbit anti-GFP is a primary antibody that binds to green fluorescent protein (GFP). This antibody can be used to detect and visualize GFP-tagged proteins in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Rabbit anti cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions) Mouse anti elav antibody, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti p fak, by Cell Signaling Technology (1 mentions) Rabbit anti jnk, by Santa Cruz Biotechnology (1 mentions) Mouse anti repo, by Developmental Studies Hybridoma Bank (1 mentions) Mouse anti aβ42 6e10, by Fortrea (1 mentions) Rat anti mouse cd16 32, by BD (1 mentions) Axio observer microscope, by Zeiss (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions) Rat anti cd45, by BD (1 mentions) Rabbit anti histone h3, by Abcam (1 mentions) Mouse anti β actin, by GeneTex (1 mentions)

Close spelling variants of this product

Rabbit anti-GFP antibody Polyclonal rabbit anti-GFP Anti-GFP

3 protocols using rabbit anti gfp

1

Immunostaining and Western Blot Protocols

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Primary antibodies and titers used in this study for immunostaining are as follows: Rabbit anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling, 1:200), mouse anti-Elav antibody (Developmental Studies Hybridoma Bank, DSHB, 1:50), mouse anti-Repo (Developmental Studies Hybridoma Bank, DSHB, 1:50), rabbit anti-GFP (GeneTex, 1:200), mouse anti-NimC1 (a gift from Dr István Andó, 1:30)24 (link), rabbit anti-Eiger (a gift from Dr Chun-Hong Chen, National Health Research institute, NHRI, 1:200), rabbit anti-FasII (a gift from Dr Vivian Budnik, 1:5000)42 (link) and rabbit anti-pFAK (Cell Signaling, 1:200). For western blotting, mouse anti-Aβ42 (6E10) (Covance, 1:5000), rabbit anti-phospho JNK (pTPpY) (Promega, 1:2000), rabbit anti-JNK (Santa Cruz, 1:5000), and rabbit anti-GFP (GeneTex, 1:5000) were used.
Wu S.C., Cao Z.S., Chang K.M, & Juang J.L. (2017). Intestinal microbial dysbiosis aggravates the progression of Alzheimer’s disease in Drosophila. Nature Communications, 8, 24.
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2

Immunofluorescence Analysis of Spinal Cord and DRG

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Spinal cord and DRG were post-fixed in 4% paraformaldehyde and cryoprotected in sucrose. Cryosections (10-μm) of lumbar DRG and of lumbar segments L2–L5 were stained. We used 1:500 rat antimouse CD16/32 (BD Bioscience) or 1:500 rabbit anti-Iba- 1 (Wako Pure Chemical Industries, Richmond, VA), 1:100 rabbit anti-GFP (GeneTex, San Antonio, TX), and 1:200 rat anti-CD45 (BD Bioscience), followed by alexafluor 488-conjugated streptavidin or alexafluor 488- or 594-conjugated secondary antibodies. Photographs were taken with a Zeiss Axio Observer microscope (Zeiss, Oberkochen, Germany).
Willemen H.L., Eijkelkamp N., Carbajal A.G., Wang H., Mack M., Zijlstra J., Heijnen C.J, & Kavelaars A. (2014). Monocytes/Macrophages Control Resolution of Transient Inflammatory Pain. The journal of pain : official journal of the American Pain Society, 15(5), 496-506.
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3

Western Blot Antibody Dilutions

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For Westerns, primary antibodies were used as the following dilutions: rabbit anti-TER94 (1:5,000), rabbit anti-Histone H3 (1:40,000, Abcam), mouse anti-β-actin (1:20,000, GeneTex), rabbit anti-GFP (1:1,000, GeneTex), rabbit anti-Mu2 (1:1,000), and rabbit anti-p53 (1:500). Secondary antibodies conjugated with HRP (Jackson ImmunoResearch Laboratories) were used in 1:10,000 dilutions. All loading controls were prepared by stripping off the reagents from the original membrane and then reimmunoblotting with anti-β-actin (for whole cell lysate and cytosolic fraction) or anti-Histone H3 (for nuclear fraction) following the standard procedures.
Chang Y., Peng Y., Shih C., Chang H., & Sang T. (2020). VCP functions to maintain nuclear size by managing DNA damage-induced MDC1–p53–autophagy axis.
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