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4 protocols using methyl orange

1

Synthesis of Graphene Oxide from Graphite Nanofibres

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By the modified Hummers method, nGO was prepared using platelet graphite nanofibres (PGNFs) as the starting material [13 (link)]. The PGNF (diameter: 50 nm) was purchased from Strem Chemicals, Inc. Potassium peroxo-disulfate and phosphorus pentoxide were purchased from Nacalai Tesque, Inc. Sorbitan monooleate (Span 80), ethylenediamine (EDA) and 2, 2′-oxybisethylamine (OBEA) were purchased from Tokyo Chemical Industry Co., Ltd. Methyl orange was purchased from FUJIFILM Wako Pure Chemical Corp. The purchased chemicals were used without further purification.
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2

Synthesis of Silicone Elastomer Composites

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Silpot 184 silicone elastomer was purchased from Dow Corning Toray Co., Ltd. Acetone, ethanol (95), ethanol (99.5), polyethylene glycol 2000 (PEG2000), heptane, hydrochloric acid, sodium hydroxide, methyl orange, p-dimethylaminobenzaldehyde, and hydrazine dihydrochloride were obtained from FUJIFILM Wako Pure Chemical Industries (Osaka, Japan). An ABS filament was obtained from WANHAO.
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3

Degradation of Dyes by B. multivorans CCA53

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CCA53 was previously deposited in the HUT culture collection under strain number HUT-8135. Bordeaux S, bromothymol blue, congo red, cresol red, curcumin, ethyl red, fluorescein, methyl orange, methyl red, orange II, rhodamine B, thymol blue, and trypan blue were purchased from Fujifilm Wako Chemicals (Osaka, Japan) or Tokyo Chemical Industry (Tokyo, Japan). To confirm dyedegradation capacity, B. multivorans CCA53 was aerobically grown on LB plates (pH 7.0) containing 100 µM each dye for 3 days at 30°C.
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4

AuNPs-based Thrombin Aptamer Assay

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AuNPs (40 nm in diameter) were obtained from BBI solutions (Cardiff, UK). Thrombin binding aptamer (Th-apt, 5′-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′) was purchased from Eurofins Genomics (Tokyo, Japan). 22 2-Melcaptethanol (2-ME) and methyl orange were obtained from Wako (Osaka, Japan). 3-Aminopropyltriethoxysilane (APTES) and bovine serum albumin (BSA) were obtained from Tokyo Chemical Industry (Tokyo, Japan) and Bio RAD (Hercules, CA, USA), respectively. Slide glass and cover glass were purchased from Matsunami Glass Industry (Osaka, Japan).
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