At 12–14 days following surgery, rats received an intraperitoneal injection of (+)-methamphetamine hydrochloride (M8750 Sigma) (3 mg/kg), and were then placed in a clean open field container (45 × 45 cm) surrounded by plexiglass walls in a quiet, well-lit room. At 30 min after injection, their activity was recorded for a 10-min period. For scoring, three investigators blind to treatment regimen counted the number of 360-degree ipsiversive rotations, as previously described.22 (link) The container was sanitized with 70% ethanol between each trial and allowed to air dry.
M8750
Manufactured by Merck Group
The M8750 is a high-performance laboratory centrifuge designed for a variety of applications. It features a versatile rotor system, digital speed and time controls, and a robust construction for reliable operation.
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Lab products found in correlation
8 protocols using m8750
1
Methamphetamine-Induced Rotational Behavior
2
Methamphetamine-induced Behavioral Sensitization in Rats
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Male Wistar rats (220±10 g) were housed in a 12 hr light/dark cycle at 22 to 24°C, with food and water ad libitum. All of the experimental procedures were conducted in accordance with the National Institutes of Health’s Guide for the Care and Use of Laboratory Animals. All efforts were made to minimize the number of animals used and their suffering. Methamphetamine hydrochloride (Sigma–Aldrich, M 8750) was dissolved in 0.9% saline. The rats were chronically treated with subcutaneous injections of METH (2 mg/kg), once a day for 5 days, as described previously (15 (link)). Rats become sensitized to METH during 5 days. Normal saline solution was similarly injected into control rats. This dose of METH shows no neurotoxicity but produces behavioral sensitization after repeated treatment in rats (16 (link)).
3
Apomorphine-Induced Rotational Behavior
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Apomorphine-induced circling test was used to reflect DA denervation in rodents with unilateral nigrostriatal lesion50 (link),52 (link). In brief, 14 days after surgery, rats were injected intraperitoneally with apomorphine (M8750 Sigma, 5 mg/kg) and placed in a clean open arena (120 × 120 cm). The whole experiment was performed in a quiet, well-lit room. Thirty minutes after the injection, the rats were recorded for 10 min. The number of rotations towards the contralateral side of lesion was counted.
4
Pharmacological Interventions in Preclinical Neuroscience
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All drugs were dissolved or diluted in 0.9% SAL and administered at a volume of 0.01 ml/g. MDMA (7.5 mg/kg; Organix Inc., O-8571) and METH (2 mg/kg; Sigma-Aldrich M8750) were administered intraperitoneally. The dose of METH was selected on the basis of previous studies (18 (link)), which found that this dose (2 mg/kg) was sufficient to induce locomotor sensitization and conditioned place preference, but not an effect in the three-chamber social preference assay. MOR (10 mg/kg; Sigma-Aldrich, M8777, MOR sulfate salt pentahydrate) and meloxicam (10 mg/kg; VetOne, Ostilox, 5 mg/ml solution) were administered subcutaneously. For TRAP experiments, 4-OHT (Sigma-Aldrich, H6278) was prepared in a mixture of castor and sunflower oil (20:80 ratio) and injected intraperitoneally at a dose of 50 mg/kg.
5
Methamphetamine-Induced Rotational Behavior
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At 12–14 days following surgery, rats received an intraperitoneal injection of (+)-methamphetamine hydrochloride (M8750 Sigma) (3 mg/kg), and were then placed in a clean open field container (45×45 cm) surrounded by plexiglass walls in a quiet, well-lit room. At 30 min after injection, their activity was recorded for a 10-min period. For scoring, three investigators blind to treatment regimen counted the number of 360-degree ipsiversive rotations, as previously described.22 (link) The container was sanitized with 70% ethanol between each trial and allowed to air dry.
6
Methamphetamine Administration in Rats
7
Methamphetamine and Norepinephrine Effects
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Reagent doses were optimized in dose-response experiments in both macrophages and BAs, to account for viability and biological effects. (+) Methamphetamine hydrochloride (M8750 – Sigma-Aldrich) was added to the culture media at 60 μM, as previously reported [6 ,7 ,23 ] and cells were harvested at indicated time-points. (−) Norepinephrine (A7257, Sigma Aldrich) was added to the cultures at 0.1 μM. N-acetyl cysteine was added at 5 pg/ml (A7250, Sigma Aldrich). ROS measurements were performed at 30 min and 24 h after stimuli. Assays were performed 24 h after stimulations unless stated in figure legends. Trypan blue (Sigma Aldrich) dye exclusion staining was performed to count cells and assess viability upon plating and retrieval. Cultures with less than 80% viability were discarded.
8
Methamphetamine and Norepinephrine Effects
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