Fluorescein labeled anti mouse antibody

Manufactured by Merck Group

Sourced in United Kingdom

Fluorescein-labeled anti-mouse antibody is a laboratory reagent used to detect and visualize mouse proteins in biological samples. The antibody is conjugated with the fluorescent dye fluorescein, which allows for the specific labeling and identification of target mouse proteins.

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Lab products found in correlation

Confocal laser scanning microscope, by Leica camera (2 mentions) Cy3 labeled anti rat igg antibody, by Merck Group (2 mentions) Neun antibody, by Merck Group (2 mentions) Fitc labeled anti mouse antibody, by Merck Group (2 mentions) Rat monoclonal anti brdu antibody, by Abcam (1 mentions) Mouse monoclonal anti gfap antibody, by Merck Group (1 mentions) Rat anti brdu antibody, by Abcam (1 mentions) Mouse anti gfap antibody, by Merck Group (1 mentions)

2 protocols using fluorescein labeled anti mouse antibody

Multifaceted Cellular Immunolabeling Assay

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The sections were incubated with rat monoclonal anti-BrdU antibody (1:200; Abcam, Cambridge, UK), which was revealed using CY3-labeled anti-rat IgG antibody (1:200) and mouse monoclonal anti-neuronal nuclei (NeuN) antibody (1:500, Millipore), which was revealed using fluorescein-labeled anti-mouse antibody (1:50) or mouse monoclonal anti-GFAP antibody (1:200, Millipore), which was revealed using FITC-labeled anti-mouse antibody (1:50). The immunopositive cells/fibers were observed by a confocal laser-scanning microscope (Leica, Heidelberg, Germany).

Li G., Zhou L., Zhu Y., Wang C., Sha S., Xian X., Ji Y., Liu G, & Chen L. (2015). Seipin knockout in mice impairs stem cell proliferation and progenitor cell differentiation in the adult hippocampal dentate gyrus via reduced levels of PPARγ. Disease Models & Mechanisms, 8(12), 1615-1624.

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Quantifying Neurogenesis and Gliogenesis

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The sections were incubated with rat anti-BrdU antibody (1:200, Abcam, Cambridge, UK), which was detected using CY3-labeled anti-rat IgG antibody (1:200, Millipore) and mouse anti-neuronal nuclei (NeuN) antibody (1:500, Millipore), which was detected using fluorescein-labeled anti-mouse antibody (1:50, Millipore) or mouse anti-GFAP antibody (1:200, Millipore), which was detected using a FITC-labeled anti-mouse antibody (1:50, Millipore). BrdU⁺/NeuN⁺ or GFAP⁺ cells were observed using a confocal laser-scanning microscope (Leica, Heidelberg, Germany). The number of BrdU⁺/NeuN⁺ or GFAP⁺ cells per section (200 μm apart) was multiplied by 5 to obtain the total number.

Zhang T., Hong J., Di T, & Chen L. (2016). MPTP Impairs Dopamine D1 Receptor-Mediated Survival of Newborn Neurons in Ventral Hippocampus to Cause Depressive-Like Behaviors in Adult Mice. Frontiers in Molecular Neuroscience, 9, 101.

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